Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection

Abstract To cope with the shortage of filtering facepiece respirators (FFRs) during the coronavirus (COVID-19) pandemic, healthcare institutions were forced to reuse FFRs after applying different decontamination methods including gamma-irradiation (GIR). The aim of this study was to evaluate the eff...

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Autores principales:	Khaled Al-Hadyan, Ghazi Alsbeih, Najla Al-Harbi, Sara Bin Judia, Maha Al-Ghamdi, Akram Almousa, Ibtihaj Alsharif, Razan Bakheet, Khaldoun Al-Romaih, Maha Al-Mozaini, Salem Al-Ghamdi, Belal Moftah, Rashed Alhmaid
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64805cae483448e8ac3e527236c9eff1
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spelling	oai:doaj.org-article:64805cae483448e8ac3e527236c9eff12021-12-02T18:37:08ZEffect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection10.1038/s41598-021-99414-62045-2322https://doaj.org/article/64805cae483448e8ac3e527236c9eff12021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99414-6https://doaj.org/toc/2045-2322Abstract To cope with the shortage of filtering facepiece respirators (FFRs) during the coronavirus (COVID-19) pandemic, healthcare institutions were forced to reuse FFRs after applying different decontamination methods including gamma-irradiation (GIR). The aim of this study was to evaluate the effect of GIR on the filtration efficiency (FE) of FFRs and on SARS-CoV-2 detection. The FE of 2 FFRs types (KN95 and N95-3 M masks) was assessed at different particle sizes (0.3–5 µm) following GIR (0–15 kGy) delivered at either typical (1.65 kGy/h) or low (0.5088 kGy/h) dose rates. The detection of two SARS-CoV-2 RNA genes (E and RdRp4) following GIR (0–50 kGy) was carried out using RT-qPCR assay. Both masks showed an overall significant (P < 0.001) reduction in FE with increased GIR doses. No significant differences were observed between GIR dose rates on FE. The GIR exhibited significant increases (P ≤ 0.001) in the cycle threshold values (ΔCt) of both genes, with no detection following high doses. In conclusion, complete degradation of SARS-CoV-2 RNA can be achieved by high GIR (≥ 30 kGy), suggesting its potential use in FFRs decontamination. However, GIR exhibited adverse effects on FE in dose- and particle size-dependent manners, rendering its use to decontaminate FFRs debatable.Khaled Al-HadyanGhazi AlsbeihNajla Al-HarbiSara Bin JudiaMaha Al-GhamdiAkram AlmousaIbtihaj AlsharifRazan BakheetKhaldoun Al-RomaihMaha Al-MozainiSalem Al-GhamdiBelal MoftahRashed AlhmaidNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khaled Al-Hadyan Ghazi Alsbeih Najla Al-Harbi Sara Bin Judia Maha Al-Ghamdi Akram Almousa Ibtihaj Alsharif Razan Bakheet Khaldoun Al-Romaih Maha Al-Mozaini Salem Al-Ghamdi Belal Moftah Rashed Alhmaid Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection
description	Abstract To cope with the shortage of filtering facepiece respirators (FFRs) during the coronavirus (COVID-19) pandemic, healthcare institutions were forced to reuse FFRs after applying different decontamination methods including gamma-irradiation (GIR). The aim of this study was to evaluate the effect of GIR on the filtration efficiency (FE) of FFRs and on SARS-CoV-2 detection. The FE of 2 FFRs types (KN95 and N95-3 M masks) was assessed at different particle sizes (0.3–5 µm) following GIR (0–15 kGy) delivered at either typical (1.65 kGy/h) or low (0.5088 kGy/h) dose rates. The detection of two SARS-CoV-2 RNA genes (E and RdRp4) following GIR (0–50 kGy) was carried out using RT-qPCR assay. Both masks showed an overall significant (P < 0.001) reduction in FE with increased GIR doses. No significant differences were observed between GIR dose rates on FE. The GIR exhibited significant increases (P ≤ 0.001) in the cycle threshold values (ΔCt) of both genes, with no detection following high doses. In conclusion, complete degradation of SARS-CoV-2 RNA can be achieved by high GIR (≥ 30 kGy), suggesting its potential use in FFRs decontamination. However, GIR exhibited adverse effects on FE in dose- and particle size-dependent manners, rendering its use to decontaminate FFRs debatable.
format	article
author	Khaled Al-Hadyan Ghazi Alsbeih Najla Al-Harbi Sara Bin Judia Maha Al-Ghamdi Akram Almousa Ibtihaj Alsharif Razan Bakheet Khaldoun Al-Romaih Maha Al-Mozaini Salem Al-Ghamdi Belal Moftah Rashed Alhmaid
author_facet	Khaled Al-Hadyan Ghazi Alsbeih Najla Al-Harbi Sara Bin Judia Maha Al-Ghamdi Akram Almousa Ibtihaj Alsharif Razan Bakheet Khaldoun Al-Romaih Maha Al-Mozaini Salem Al-Ghamdi Belal Moftah Rashed Alhmaid
author_sort	Khaled Al-Hadyan
title	Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection
title_short	Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection
title_full	Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection
title_fullStr	Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection
title_full_unstemmed	Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection
title_sort	effect of gamma irradiation on filtering facepiece respirators and sars-cov-2 detection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/64805cae483448e8ac3e527236c9eff1
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Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection

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