Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum

Abstract Non-lethal methods for semen collection from elasmobranchs to better understand species reproduction has accompanied the development of artificial insemination. Ejaculates (n = 82) collected from whitespotted bamboo sharks Chiloscyllium plagiosum (n = 19) were assessed and cold-stored raw o...

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Autores principales:	Jennifer T. Wyffels, Lance M. Adams, Frank Bulman, Ari Fustukjian, Michael W. Hyatt, Kevin A. Feldheim, Linda M. Penfold
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6483cd7de75442d3b88866580f6bcefb
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spelling	oai:doaj.org-article:6483cd7de75442d3b88866580f6bcefb2021-12-02T15:43:24ZArtificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum10.1038/s41598-021-88568-y2045-2322https://doaj.org/article/6483cd7de75442d3b88866580f6bcefb2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88568-yhttps://doaj.org/toc/2045-2322Abstract Non-lethal methods for semen collection from elasmobranchs to better understand species reproduction has accompanied the development of artificial insemination. Ejaculates (n = 82) collected from whitespotted bamboo sharks Chiloscyllium plagiosum (n = 19) were assessed and cold-stored raw or extended at 4 °C. Females (n = 20) were inseminated with fresh or 24–48 h cold-stored raw or extended semen and paternity of offspring determined with microsatellite markers. Insemination of females with fresh semen (n = 10) resulted in 80 hatchlings and 27.6% fertility. Insemination of females with semen cold-stored 24 h (n = 4) and 48 h (n = 1) semen resulted in 17 hatchlings and fertilization rates of 28.1% and 7.1% respectively. Two females inseminated with fresh or cold-stored semen laid eggs that hatched from fertilization and parthenogenesis within the same clutch. Parthenogenesis rate for inseminated females was 0.71%. Results demonstrate artificial insemination with cold-stored semen can provide a strategy for transport of male genetics nationally and internationally, precluding the need to transport sharks. Production of parthenotes in the same clutch as sexually fertilized eggs highlights the prevalence of parthenogenesis in whitespotted bamboo sharks and poses important considerations for population management.Jennifer T. WyffelsLance M. AdamsFrank BulmanAri FustukjianMichael W. HyattKevin A. FeldheimLinda M. PenfoldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer T. Wyffels Lance M. Adams Frank Bulman Ari Fustukjian Michael W. Hyatt Kevin A. Feldheim Linda M. Penfold Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum
description	Abstract Non-lethal methods for semen collection from elasmobranchs to better understand species reproduction has accompanied the development of artificial insemination. Ejaculates (n = 82) collected from whitespotted bamboo sharks Chiloscyllium plagiosum (n = 19) were assessed and cold-stored raw or extended at 4 °C. Females (n = 20) were inseminated with fresh or 24–48 h cold-stored raw or extended semen and paternity of offspring determined with microsatellite markers. Insemination of females with fresh semen (n = 10) resulted in 80 hatchlings and 27.6% fertility. Insemination of females with semen cold-stored 24 h (n = 4) and 48 h (n = 1) semen resulted in 17 hatchlings and fertilization rates of 28.1% and 7.1% respectively. Two females inseminated with fresh or cold-stored semen laid eggs that hatched from fertilization and parthenogenesis within the same clutch. Parthenogenesis rate for inseminated females was 0.71%. Results demonstrate artificial insemination with cold-stored semen can provide a strategy for transport of male genetics nationally and internationally, precluding the need to transport sharks. Production of parthenotes in the same clutch as sexually fertilized eggs highlights the prevalence of parthenogenesis in whitespotted bamboo sharks and poses important considerations for population management.
format	article
author	Jennifer T. Wyffels Lance M. Adams Frank Bulman Ari Fustukjian Michael W. Hyatt Kevin A. Feldheim Linda M. Penfold
author_facet	Jennifer T. Wyffels Lance M. Adams Frank Bulman Ari Fustukjian Michael W. Hyatt Kevin A. Feldheim Linda M. Penfold
author_sort	Jennifer T. Wyffels
title	Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum
title_short	Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum
title_full	Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum
title_fullStr	Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum
title_full_unstemmed	Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum
title_sort	artificial insemination and parthenogenesis in the whitespotted bamboo shark chiloscyllium plagiosum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6483cd7de75442d3b88866580f6bcefb
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Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum

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