Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s

Abstract To trace the linkage between Japanese healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) strains in the early 1980s and the 2000s onward, we performed molecular characterizations using mainly whole-genome sequencing. Among the 194 S. aureus strains isolated, 20 mecA...

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Autores principales:	Hui Zuo, Yuki Uehara, Yujie Lu, Takashi Sasaki, Keiichi Hiramatsu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64887d7376094088b805a1d4dcf9f42e
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spelling	oai:doaj.org-article:64887d7376094088b805a1d4dcf9f42e2021-12-02T13:20:12ZGenetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s10.1038/s41598-021-84481-62045-2322https://doaj.org/article/64887d7376094088b805a1d4dcf9f42e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84481-6https://doaj.org/toc/2045-2322Abstract To trace the linkage between Japanese healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) strains in the early 1980s and the 2000s onward, we performed molecular characterizations using mainly whole-genome sequencing. Among the 194 S. aureus strains isolated, 20 mecA-positive MRSA (10.3%), 8 mecA-negative MRSA (4.1%) and 3 mecA-positive methicillin-susceptible S. aureus (MSSA) (1.5%) strains were identified. The most frequent sequence type (ST) was ST30 (n = 11), followed by ST5 (n = 8), ST81 (n = 4), and ST247 (n = 3). Rates of staphylococcal cassette chromosome mec (SCCmec) types I, II, and IV composed 65.2%, 13.0%, and 17.4% of isolates, respectively. Notably, 73.3% of SCCmec type I strains were susceptible to imipenem unlike SCCmec type II strains (0%). ST30-SCCmec I (n = 7) and ST5-SCCmec I (n = 5) predominated, whereas only two strains exhibited imipenem-resistance and were tst-positive ST5-SCCmec II, which is the current Japanese HA-MRSA genotype. All ST30 strains shared the common ancestor strain 55/2053, which caused the global pandemic of Panton-Valentine leukocidin-positive MSSA in Europe and the United States in the 1950s. Conspicuously more heterogeneous, the population of HA-MRSA clones observed in the 1980s, including the ST30-SCCmec I clone, has shifted to the current homogeneous population of imipenem-resistant ST5-SCCmec II clones, probably due to the introduction of new antimicrobials.Hui ZuoYuki UeharaYujie LuTakashi SasakiKeiichi HiramatsuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Zuo Yuki Uehara Yujie Lu Takashi Sasaki Keiichi Hiramatsu Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s
description	Abstract To trace the linkage between Japanese healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) strains in the early 1980s and the 2000s onward, we performed molecular characterizations using mainly whole-genome sequencing. Among the 194 S. aureus strains isolated, 20 mecA-positive MRSA (10.3%), 8 mecA-negative MRSA (4.1%) and 3 mecA-positive methicillin-susceptible S. aureus (MSSA) (1.5%) strains were identified. The most frequent sequence type (ST) was ST30 (n = 11), followed by ST5 (n = 8), ST81 (n = 4), and ST247 (n = 3). Rates of staphylococcal cassette chromosome mec (SCCmec) types I, II, and IV composed 65.2%, 13.0%, and 17.4% of isolates, respectively. Notably, 73.3% of SCCmec type I strains were susceptible to imipenem unlike SCCmec type II strains (0%). ST30-SCCmec I (n = 7) and ST5-SCCmec I (n = 5) predominated, whereas only two strains exhibited imipenem-resistance and were tst-positive ST5-SCCmec II, which is the current Japanese HA-MRSA genotype. All ST30 strains shared the common ancestor strain 55/2053, which caused the global pandemic of Panton-Valentine leukocidin-positive MSSA in Europe and the United States in the 1950s. Conspicuously more heterogeneous, the population of HA-MRSA clones observed in the 1980s, including the ST30-SCCmec I clone, has shifted to the current homogeneous population of imipenem-resistant ST5-SCCmec II clones, probably due to the introduction of new antimicrobials.
format	article
author	Hui Zuo Yuki Uehara Yujie Lu Takashi Sasaki Keiichi Hiramatsu
author_facet	Hui Zuo Yuki Uehara Yujie Lu Takashi Sasaki Keiichi Hiramatsu
author_sort	Hui Zuo
title	Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s
title_short	Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s
title_full	Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s
title_fullStr	Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s
title_full_unstemmed	Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s
title_sort	genetic and phenotypic diversity of methicillin-resistant staphylococcus aureus among japanese inpatients in the early 1980s
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/64887d7376094088b805a1d4dcf9f42e
work_keys_str_mv	AT huizuo geneticandphenotypicdiversityofmethicillinresistantstaphylococcusaureusamongjapaneseinpatientsintheearly1980s AT yukiuehara geneticandphenotypicdiversityofmethicillinresistantstaphylococcusaureusamongjapaneseinpatientsintheearly1980s AT yujielu geneticandphenotypicdiversityofmethicillinresistantstaphylococcusaureusamongjapaneseinpatientsintheearly1980s AT takashisasaki geneticandphenotypicdiversityofmethicillinresistantstaphylococcusaureusamongjapaneseinpatientsintheearly1980s AT keiichihiramatsu geneticandphenotypicdiversityofmethicillinresistantstaphylococcusaureusamongjapaneseinpatientsintheearly1980s
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Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s

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