Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers

In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical arms, each containing a pre-tr...

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Autores principales:	Tania Pomili, Paolo Donati, Pier Paolo Pompa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	point-of-care paper-based multiplexing colorimetric test saliva Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/648fc7db380c4d6497783d04dce232d4
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id	oai:doaj.org-article:648fc7db380c4d6497783d04dce232d4
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spelling	oai:doaj.org-article:648fc7db380c4d6497783d04dce232d42021-11-25T16:55:28ZPaper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers10.3390/bios111104432079-6374https://doaj.org/article/648fc7db380c4d6497783d04dce232d42021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/443https://doaj.org/toc/2079-6374In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical arms, each containing a pre-treatment and test zone. Its one-step fabrication is realized by CO<sub>2</sub> laser cutting, providing remarkable parallelization and rapidity (ca. 5 s/device). The colorimetric detection is based on the sensitive and selective target-induced reshaping of plasmonic multibranched gold nanoparticles, which exhibit a clear spectral shift (and blue-to-pink color change) in case of non-physiological concentrations of the three salivary biomarkers. A rapid and multiplexed naked-eye or smartphone-based readout of the colorimetric response is achieved within 10 min. A prototype kit for POCT testing is also reported, providing robustness and easy handling of the device.Tania PomiliPaolo DonatiPier Paolo PompaMDPI AGarticlepoint-of-carepaper-basedmultiplexingcolorimetric testsalivaBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 443, p 443 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	point-of-care paper-based multiplexing colorimetric test saliva Biotechnology TP248.13-248.65
spellingShingle	point-of-care paper-based multiplexing colorimetric test saliva Biotechnology TP248.13-248.65 Tania Pomili Paolo Donati Pier Paolo Pompa Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
description	In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical arms, each containing a pre-treatment and test zone. Its one-step fabrication is realized by CO<sub>2</sub> laser cutting, providing remarkable parallelization and rapidity (ca. 5 s/device). The colorimetric detection is based on the sensitive and selective target-induced reshaping of plasmonic multibranched gold nanoparticles, which exhibit a clear spectral shift (and blue-to-pink color change) in case of non-physiological concentrations of the three salivary biomarkers. A rapid and multiplexed naked-eye or smartphone-based readout of the colorimetric response is achieved within 10 min. A prototype kit for POCT testing is also reported, providing robustness and easy handling of the device.
format	article
author	Tania Pomili Paolo Donati Pier Paolo Pompa
author_facet	Tania Pomili Paolo Donati Pier Paolo Pompa
author_sort	Tania Pomili
title	Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
title_short	Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
title_full	Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
title_fullStr	Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
title_full_unstemmed	Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
title_sort	paper-based multiplexed colorimetric device for the simultaneous detection of salivary biomarkers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/648fc7db380c4d6497783d04dce232d4
work_keys_str_mv	AT taniapomili paperbasedmultiplexedcolorimetricdeviceforthesimultaneousdetectionofsalivarybiomarkers AT paolodonati paperbasedmultiplexedcolorimetricdeviceforthesimultaneousdetectionofsalivarybiomarkers AT pierpaolopompa paperbasedmultiplexedcolorimetricdeviceforthesimultaneousdetectionofsalivarybiomarkers
_version_	1718412839597637632

Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers

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