The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)

Environmental DNA (eDNA) is nuclear or mitochondrial DNA shed into the environment, and amplifying this DNA can serve as a reliable, noninvasive way to monitor aquatic systems for the presence of an invasive species. Assays based on the collection of eDNA are becoming increasingly popular, and, when...

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Autores principales:	Stephanie S. Coster, Megan N. Dillon, William Moore, George T. Merovich
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/648fd8a9c1bb4823afd531f360f35b14
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spelling	oai:doaj.org-article:648fd8a9c1bb4823afd531f360f35b142021-11-04T07:42:09ZThe update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)1932-6203https://doaj.org/article/648fd8a9c1bb4823afd531f360f35b142021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555798/?tool=EBIhttps://doaj.org/toc/1932-6203Environmental DNA (eDNA) is nuclear or mitochondrial DNA shed into the environment, and amplifying this DNA can serve as a reliable, noninvasive way to monitor aquatic systems for the presence of an invasive species. Assays based on the collection of eDNA are becoming increasingly popular, and, when optimized, can aid in effectively and efficiently tracking invasion fronts. We set out to update an eDNA assay to detect the invasive rusty crayfish, Faxonius rusticus. We tested for species specificity compared to other stream crayfish and field tested the assay at sites with known presence (N = 3) and absence (N = 4) in the Juniata River watershed in central Pennsylvania, USA. To maximize sensitivity, we field tested different storage buffers (Longmire’s buffer and ethanol), DNA extraction methods (Qiagen’s DNEasy and PowerWater kits), and quantitative polymerase chain reaction (qPCR) chemistries (TaqMan and SYBR green). Our assay confirmed the presence data and performed optimally when filter samples were stored in Longmire’s buffer, DNA was extracted with DNeasy Blood and Tissue Kit, and TaqMan qPCR chemistry was utilized. With proper sample processing, our assay allows for accurate, noninvasive detection of F. rusticus in streams.Stephanie S. CosterMegan N. DillonWilliam MooreGeorge T. MerovichPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephanie S. Coster Megan N. Dillon William Moore George T. Merovich The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)
description	Environmental DNA (eDNA) is nuclear or mitochondrial DNA shed into the environment, and amplifying this DNA can serve as a reliable, noninvasive way to monitor aquatic systems for the presence of an invasive species. Assays based on the collection of eDNA are becoming increasingly popular, and, when optimized, can aid in effectively and efficiently tracking invasion fronts. We set out to update an eDNA assay to detect the invasive rusty crayfish, Faxonius rusticus. We tested for species specificity compared to other stream crayfish and field tested the assay at sites with known presence (N = 3) and absence (N = 4) in the Juniata River watershed in central Pennsylvania, USA. To maximize sensitivity, we field tested different storage buffers (Longmire’s buffer and ethanol), DNA extraction methods (Qiagen’s DNEasy and PowerWater kits), and quantitative polymerase chain reaction (qPCR) chemistries (TaqMan and SYBR green). Our assay confirmed the presence data and performed optimally when filter samples were stored in Longmire’s buffer, DNA was extracted with DNeasy Blood and Tissue Kit, and TaqMan qPCR chemistry was utilized. With proper sample processing, our assay allows for accurate, noninvasive detection of F. rusticus in streams.
format	article
author	Stephanie S. Coster Megan N. Dillon William Moore George T. Merovich
author_facet	Stephanie S. Coster Megan N. Dillon William Moore George T. Merovich
author_sort	Stephanie S. Coster
title	The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)
title_short	The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)
title_full	The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)
title_fullStr	The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)
title_full_unstemmed	The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)
title_sort	update and optimization of an edna assay to detect the invasive rusty crayfish (faxonius rusticus)
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/648fd8a9c1bb4823afd531f360f35b14
work_keys_str_mv	AT stephaniescoster theupdateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus AT meganndillon theupdateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus AT williammoore theupdateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus AT georgetmerovich theupdateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus AT stephaniescoster updateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus AT meganndillon updateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus AT williammoore updateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus AT georgetmerovich updateandoptimizationofanednaassaytodetecttheinvasiverustycrayfishfaxoniusrusticus
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The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)

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