LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma
Background: Lymphoid-restricted membrane protein (LRMP) is an endoplasmic reticulum-associated protein that is expressed in a developmentally regulated manner in both B and T cell lineages. However, the role of LRMP in the growth, prognosis and immune infiltration in lung adenocarcinoma (LUAD) remai...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/649777577b924a01865b4150816dffac |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:649777577b924a01865b4150816dffac |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:649777577b924a01865b4150816dffac2021-12-01T07:10:07ZLRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma2296-889X10.3389/fmolb.2021.711928https://doaj.org/article/649777577b924a01865b4150816dffac2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.711928/fullhttps://doaj.org/toc/2296-889XBackground: Lymphoid-restricted membrane protein (LRMP) is an endoplasmic reticulum-associated protein that is expressed in a developmentally regulated manner in both B and T cell lineages. However, the role of LRMP in the growth, prognosis and immune infiltration in lung adenocarcinoma (LUAD) remains unclear.Method: The expression levels of LRMP mRNA in tumor and normal tissues were analyzed using Tumor Immune Estimation Resource 2.0 (TIMER 2.0) and Gene Expression Profiling Interactive Analysis 2 (GEPIA 2). LRMP protein expression was examined using the Human Protein Atlas. In vitro experiments, including qRT-PCR Western blot and immunohistochemistry staining were also performed to investigate LRMP expression. GEPIA2 and Kaplan-Meier plotter databases were used to analyze the clinical prognostic significance of LRMP. To further confirm the underlying function of LRMP, the data were analyzed using gene set enrichment analysis. Moreover, we also constructed plasmids to overexpress LRMP and explored the effect of LRMP in A549 cell line. Additionally, Tumor Immune single-cell Hub was used to investigate the distribution of LRMP in the LUAD immune microenvironment; TIMER and CIBERSORT were used to investigate the relationships among LRMP, LRMP co-expressed genes, and tumor-infiltrating immune cells; Finally, the correlations between LRMP and immune checkpoints were analyzed using TIMER 2.0.Results: The expression of LRMP was significantly lower in LUAD tissues and cell lines. High LRMP expression is associated with a better prognosis in patients with LUAD. In vitro experimental studies demonstrated that overexpression of LRMP could decrease the proliferation, migration and invasion in A549 cells, and downregulated multiple oncogenic signaling pathways, including p-STAT3, p-PI3K-p-AKT, p-MEK and EMT pathways. GSEA results showed that immuno-related and cell adhesion pathways were enriched in samples with high LRMP expression. LRMP and its co-expressed genes were positively correlated with various tumor-infiltrating immune cells and their markers. Additionally, LRMP positively correlated with immune checkpoints.Conclusions: Our data suggest that LRMP may act as a tumor suppressor gene and indicates a better prognosis. Moreover, LRMP is associated with immune infiltrates which may be involved in immunotherapy response in LUAD. Further studies are needed to validate these findings.Xin JinXin JinLiwei ChenNing ZhouNing ZhouHong NiHong NiLingling ZuLingling ZuJinling HeJinling HeLingqi YangLingqi YangYifan ZhuYifan ZhuXiaoyue SunXiaoyue SunXiaojiang LiSong XuSong XuFrontiers Media S.A.articleLRMPlung adenocarcinomaprognosisimmune infiltrationimmune checkpoints (ICP)Biology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
LRMP lung adenocarcinoma prognosis immune infiltration immune checkpoints (ICP) Biology (General) QH301-705.5 |
| spellingShingle |
LRMP lung adenocarcinoma prognosis immune infiltration immune checkpoints (ICP) Biology (General) QH301-705.5 Xin Jin Xin Jin Liwei Chen Ning Zhou Ning Zhou Hong Ni Hong Ni Lingling Zu Lingling Zu Jinling He Jinling He Lingqi Yang Lingqi Yang Yifan Zhu Yifan Zhu Xiaoyue Sun Xiaoyue Sun Xiaojiang Li Song Xu Song Xu LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma |
| description |
Background: Lymphoid-restricted membrane protein (LRMP) is an endoplasmic reticulum-associated protein that is expressed in a developmentally regulated manner in both B and T cell lineages. However, the role of LRMP in the growth, prognosis and immune infiltration in lung adenocarcinoma (LUAD) remains unclear.Method: The expression levels of LRMP mRNA in tumor and normal tissues were analyzed using Tumor Immune Estimation Resource 2.0 (TIMER 2.0) and Gene Expression Profiling Interactive Analysis 2 (GEPIA 2). LRMP protein expression was examined using the Human Protein Atlas. In vitro experiments, including qRT-PCR Western blot and immunohistochemistry staining were also performed to investigate LRMP expression. GEPIA2 and Kaplan-Meier plotter databases were used to analyze the clinical prognostic significance of LRMP. To further confirm the underlying function of LRMP, the data were analyzed using gene set enrichment analysis. Moreover, we also constructed plasmids to overexpress LRMP and explored the effect of LRMP in A549 cell line. Additionally, Tumor Immune single-cell Hub was used to investigate the distribution of LRMP in the LUAD immune microenvironment; TIMER and CIBERSORT were used to investigate the relationships among LRMP, LRMP co-expressed genes, and tumor-infiltrating immune cells; Finally, the correlations between LRMP and immune checkpoints were analyzed using TIMER 2.0.Results: The expression of LRMP was significantly lower in LUAD tissues and cell lines. High LRMP expression is associated with a better prognosis in patients with LUAD. In vitro experimental studies demonstrated that overexpression of LRMP could decrease the proliferation, migration and invasion in A549 cells, and downregulated multiple oncogenic signaling pathways, including p-STAT3, p-PI3K-p-AKT, p-MEK and EMT pathways. GSEA results showed that immuno-related and cell adhesion pathways were enriched in samples with high LRMP expression. LRMP and its co-expressed genes were positively correlated with various tumor-infiltrating immune cells and their markers. Additionally, LRMP positively correlated with immune checkpoints.Conclusions: Our data suggest that LRMP may act as a tumor suppressor gene and indicates a better prognosis. Moreover, LRMP is associated with immune infiltrates which may be involved in immunotherapy response in LUAD. Further studies are needed to validate these findings. |
| format |
article |
| author |
Xin Jin Xin Jin Liwei Chen Ning Zhou Ning Zhou Hong Ni Hong Ni Lingling Zu Lingling Zu Jinling He Jinling He Lingqi Yang Lingqi Yang Yifan Zhu Yifan Zhu Xiaoyue Sun Xiaoyue Sun Xiaojiang Li Song Xu Song Xu |
| author_facet |
Xin Jin Xin Jin Liwei Chen Ning Zhou Ning Zhou Hong Ni Hong Ni Lingling Zu Lingling Zu Jinling He Jinling He Lingqi Yang Lingqi Yang Yifan Zhu Yifan Zhu Xiaoyue Sun Xiaoyue Sun Xiaojiang Li Song Xu Song Xu |
| author_sort |
Xin Jin |
| title |
LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma |
| title_short |
LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma |
| title_full |
LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma |
| title_fullStr |
LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma |
| title_full_unstemmed |
LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma |
| title_sort |
lrmp associates with immune infiltrates and acts as a prognostic biomarker in lung adenocarcinoma |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/649777577b924a01865b4150816dffac |
| work_keys_str_mv |
AT xinjin lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT xinjin lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT liweichen lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT ningzhou lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT ningzhou lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT hongni lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT hongni lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT linglingzu lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT linglingzu lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT jinlinghe lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT jinlinghe lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT lingqiyang lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT lingqiyang lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT yifanzhu lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT yifanzhu lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT xiaoyuesun lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT xiaoyuesun lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT xiaojiangli lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT songxu lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma AT songxu lrmpassociateswithimmuneinfiltratesandactsasaprognosticbiomarkerinlungadenocarcinoma |
| _version_ |
1718405415773929472 |