IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS

Significant incidence of chronic urogenital diseases, both of viral and bacterial origin in women of reproductive age leads to an increase in the frequency of intrauterine infections. These disorders lead to disturbances of postnatal adaptation of newborns and increased numbers of infectious complic...

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Autores principales:	I. I. Remizova, G. N. Chistyakova, I. A. Gazieva, V. A. Lyapunov, L. S. Ustyantseva
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2015
Materias:	umbilical cord blood immunological parameters urogenital infection Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/649cf8d80a4d453b9476b1482a468bf5
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spelling	oai:doaj.org-article:649cf8d80a4d453b9476b1482a468bf52021-11-18T08:03:44ZIMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS1563-06252313-741X10.15789/1563-0625-2015-3-253-260https://doaj.org/article/649cf8d80a4d453b9476b1482a468bf52015-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/890https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XSignificant incidence of chronic urogenital diseases, both of viral and bacterial origin in women of reproductive age leads to an increase in the frequency of intrauterine infections. These disorders lead to disturbances of postnatal adaptation of newborns and increased numbers of infectious complications. In order to assess the state of immune system, we examined forty-eight children born to women with urogenital infection, including twenty-nine infants with complicated course of early adaptation period (study group) and twenty children diagnosed as healthy upon discharge by the day 3 to 5 after birth (comparison group). By means of flow cytometry, the following subpopulations were assayed in cord blood: CD3+, CD3+CD4+, CD3+CD8+, CD19+, CD3CD16+CD56+ cells, expression levels of markers specific to activated monocytes CD14 (HLA-DR), and lymphocytes (CD25+, CD69+), like as markers of functionally mature cells (CD45R0, CD45RA), both in total lymphocyte pool and among T cell populations. Contents of IL-1β, TNFα, IFNγ, IL-4, IL-6, IL-8, IL-10 were assessed by ELISA technique. Our studies have shown that umbilical cord blood samples from the main group of newborns showed a statistically significant decrease in relative content of CD3+, CD4+, CD8+ and CD4+CD25+ cells, along with increase in the absolute numbers of leukocytes and lymphocytes, B-cells and NK cells, as well as expression of CD45RO+, CD45RA+ and CD3+CD45RO+ receptors, CD69+ and CD14+HLADR+ activation markers, accompanied by a significant increase of IL-8 production. Numerical determination of CD45RO+, CD3+CD45RO+, CD69+ and CD14+HLA-DR+ cells, and IL-8 contents in newborns, may serve as prognostic criteria for assignment of risk group for early adaptation disturbances and development of infectious diseases.I. I. RemizovaG. N. ChistyakovaI. A. GazievaV. A. LyapunovL. S. UstyantsevaSPb RAACIarticleumbilical cord bloodimmunological parametersurogenital infectionImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 3, Pp 253-260 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	umbilical cord blood immunological parameters urogenital infection Immunologic diseases. Allergy RC581-607
spellingShingle	umbilical cord blood immunological parameters urogenital infection Immunologic diseases. Allergy RC581-607 I. I. Remizova G. N. Chistyakova I. A. Gazieva V. A. Lyapunov L. S. Ustyantseva IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS
description	Significant incidence of chronic urogenital diseases, both of viral and bacterial origin in women of reproductive age leads to an increase in the frequency of intrauterine infections. These disorders lead to disturbances of postnatal adaptation of newborns and increased numbers of infectious complications. In order to assess the state of immune system, we examined forty-eight children born to women with urogenital infection, including twenty-nine infants with complicated course of early adaptation period (study group) and twenty children diagnosed as healthy upon discharge by the day 3 to 5 after birth (comparison group). By means of flow cytometry, the following subpopulations were assayed in cord blood: CD3+, CD3+CD4+, CD3+CD8+, CD19+, CD3CD16+CD56+ cells, expression levels of markers specific to activated monocytes CD14 (HLA-DR), and lymphocytes (CD25+, CD69+), like as markers of functionally mature cells (CD45R0, CD45RA), both in total lymphocyte pool and among T cell populations. Contents of IL-1β, TNFα, IFNγ, IL-4, IL-6, IL-8, IL-10 were assessed by ELISA technique. Our studies have shown that umbilical cord blood samples from the main group of newborns showed a statistically significant decrease in relative content of CD3+, CD4+, CD8+ and CD4+CD25+ cells, along with increase in the absolute numbers of leukocytes and lymphocytes, B-cells and NK cells, as well as expression of CD45RO+, CD45RA+ and CD3+CD45RO+ receptors, CD69+ and CD14+HLADR+ activation markers, accompanied by a significant increase of IL-8 production. Numerical determination of CD45RO+, CD3+CD45RO+, CD69+ and CD14+HLA-DR+ cells, and IL-8 contents in newborns, may serve as prognostic criteria for assignment of risk group for early adaptation disturbances and development of infectious diseases.
format	article
author	I. I. Remizova G. N. Chistyakova I. A. Gazieva V. A. Lyapunov L. S. Ustyantseva
author_facet	I. I. Remizova G. N. Chistyakova I. A. Gazieva V. A. Lyapunov L. S. Ustyantseva
author_sort	I. I. Remizova
title	IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS
title_short	IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS
title_full	IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS
title_fullStr	IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS
title_full_unstemmed	IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS
title_sort	immunological parametrs of umbilical cord blood from children born to women with urogenital infections
publisher	SPb RAACI
publishDate	2015
url	https://doaj.org/article/649cf8d80a4d453b9476b1482a468bf5
work_keys_str_mv	AT iiremizova immunologicalparametrsofumbilicalcordbloodfromchildrenborntowomenwithurogenitalinfections AT gnchistyakova immunologicalparametrsofumbilicalcordbloodfromchildrenborntowomenwithurogenitalinfections AT iagazieva immunologicalparametrsofumbilicalcordbloodfromchildrenborntowomenwithurogenitalinfections AT valyapunov immunologicalparametrsofumbilicalcordbloodfromchildrenborntowomenwithurogenitalinfections AT lsustyantseva immunologicalparametrsofumbilicalcordbloodfromchildrenborntowomenwithurogenitalinfections
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IMMUNOLOGICAL PARAMETRS OF UMBILICAL CORD BLOOD FROM CHILDREN BORN TO WOMEN WITH UROGENITAL INFECTIONS

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