Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver
Abstract Liver granulomatous inflammation and fibrosis were the primary pathological changes observed during Schistosoma japonicum (S. japonicum) infection. In the present study, the characteristics of IL-9 were investigated in the liver of S. japonicum infection C57BL/6 mice. Immunofluorescence, qR...
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2017
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oai:doaj.org-article:64a30cac65d5456c8a27e45b5120ae5d2021-12-02T12:30:26ZCharacteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver10.1038/s41598-017-02422-82045-2322https://doaj.org/article/64a30cac65d5456c8a27e45b5120ae5d2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02422-8https://doaj.org/toc/2045-2322Abstract Liver granulomatous inflammation and fibrosis were the primary pathological changes observed during Schistosoma japonicum (S. japonicum) infection. In the present study, the characteristics of IL-9 were investigated in the liver of S. japonicum infection C57BL/6 mice. Immunofluorescence, qRT-PCR, and ELISA results demonstrated that the expression of IL-9 significantly increased after infection (P < 0.01). FACS results indicated that the peak of IL-9+ Th9 cells in the liver mononuclear cells appeared at the early phase of infection (week 5), except that Th9 cells, CD8+ Tc cells, NKT and γδT cells could secrete IL-9 in this model. Although IL-9 neutralization has a limited effect on liver granulomatous inflammation, it could decrease the level of fibrosis-associated factor, PC-III, in the serum of infected mice (P < 0.05). Taken together, our results indicated that IL-9 was an important type of cytokine involved in the progression of S. japonicum infection-induced hepatic damage.Lu LiHongyan XieMei WangJiale QuHefei ChaQuan YangYuanfa FengYanwei QiHuaina QiuNuo DongJun HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
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Medicine R Science Q Lu Li Hongyan Xie Mei Wang Jiale Qu Hefei Cha Quan Yang Yuanfa Feng Yanwei Qi Huaina Qiu Nuo Dong Jun Huang Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver |
| description |
Abstract Liver granulomatous inflammation and fibrosis were the primary pathological changes observed during Schistosoma japonicum (S. japonicum) infection. In the present study, the characteristics of IL-9 were investigated in the liver of S. japonicum infection C57BL/6 mice. Immunofluorescence, qRT-PCR, and ELISA results demonstrated that the expression of IL-9 significantly increased after infection (P < 0.01). FACS results indicated that the peak of IL-9+ Th9 cells in the liver mononuclear cells appeared at the early phase of infection (week 5), except that Th9 cells, CD8+ Tc cells, NKT and γδT cells could secrete IL-9 in this model. Although IL-9 neutralization has a limited effect on liver granulomatous inflammation, it could decrease the level of fibrosis-associated factor, PC-III, in the serum of infected mice (P < 0.05). Taken together, our results indicated that IL-9 was an important type of cytokine involved in the progression of S. japonicum infection-induced hepatic damage. |
| format |
article |
| author |
Lu Li Hongyan Xie Mei Wang Jiale Qu Hefei Cha Quan Yang Yuanfa Feng Yanwei Qi Huaina Qiu Nuo Dong Jun Huang |
| author_facet |
Lu Li Hongyan Xie Mei Wang Jiale Qu Hefei Cha Quan Yang Yuanfa Feng Yanwei Qi Huaina Qiu Nuo Dong Jun Huang |
| author_sort |
Lu Li |
| title |
Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver |
| title_short |
Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver |
| title_full |
Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver |
| title_fullStr |
Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver |
| title_full_unstemmed |
Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver |
| title_sort |
characteristics of il-9 induced by schistosoma japonicum infection in c57bl/6 mouse liver |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/64a30cac65d5456c8a27e45b5120ae5d |
| work_keys_str_mv |
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