Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes

Yiwen Fan1, Alexander C Pauer1, Arthur A Gonzales1, Hicham Fenniri1–3 1Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; 2Department of Bioengineering, Northeastern University, Boston, MA 02115, USA; 3Department of Chemistry and Chemical Biology, Northeast...

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Autores principales:	Fan Y, Pauer AC, Gonzales AA, Fenniri H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	ampicillin rosette nanotubes gold nanoparticles S. aureus methicillin-resistant S. aureus. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/64a852fc426541e8b755ef6874be537e
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spelling	oai:doaj.org-article:64a852fc426541e8b755ef6874be537e2021-12-02T09:08:36ZEnhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes1178-2013https://doaj.org/article/64a852fc426541e8b755ef6874be537e2019-09-01T00:00:00Zhttps://www.dovepress.com/enhanced-antibiotic-activity-of-ampicillin-conjugated-to-gold-nanopart-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yiwen Fan1, Alexander C Pauer1, Arthur A Gonzales1, Hicham Fenniri1–3 1Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; 2Department of Bioengineering, Northeastern University, Boston, MA 02115, USA; 3Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USACorrespondence: Hicham FenniriDepartment of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USATel +1 617 373 7690Fax +1 617 373 2209Email h.fenniri@northeastern.eduPurpose: This work presents the preparation of a nanocomposite of ampicillin-conjugated gold nanoparticles (AuNPs) and self-assembled rosette nanotubes (RNTs), and evaluates its antibacterial properties against two strains of drug-resistant bacteria (Staphylococcus aureus [S. aureus], methicillin-resistant S. aureus [MRSA]).Materials and methods: Small, nearly monodisperse AuNPs (1.43±0.5 nm in diameter) nucleated on the surface of polyethylene glycol-functionalized RNTs in a one-pot reaction. Upon conjugation with ampicillin, their diameter increased to 1.86±0.32 nm. The antibacterial activity of the nanocomposite against S. aureus and MRSA was tested using different concentrations of ampicillin. The cytocompatibility of the nanocomposite was also tested against human dermal fibroblasts.Results: Based on bacterial inhibition studies, the nanocomposite demonstrated enhanced antibiotic activity against both bacterial strains. The minimum inhibitory concentration (MIC) of the nanocomposite against S. aureus was found to be 0.58 μg/mL, which was 18% lower than ampicillin alone. The nanocomposite also exhibited a 20 hrs MIC of 4 μg/mL against MRSA, approximately 10–20 times lower than previously reported values for ampicillin alone. In addition, at concentrations of 4 μg/mL of ampicillin (70 μg/mL of AuNPs), the nanocomposite showed negligible cytotoxic effects.Conclusion: Our findings offer a new approach for the treatment of drug-resistant bacteria by potentiating inhibitory effects of existing antibiotics, and delivering them using a non-toxic formulation.Keywords: ampicillin, rosette nanotubes, gold nanoparticles, S. aureus, methicillin-resistant S. aureusFan YPauer ACGonzales AAFenniri HDove Medical Pressarticleampicillinrosette nanotubesgold nanoparticlesS. aureusmethicillin-resistant S. aureus.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 7281-7289 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ampicillin rosette nanotubes gold nanoparticles S. aureus methicillin-resistant S. aureus. Medicine (General) R5-920
spellingShingle	ampicillin rosette nanotubes gold nanoparticles S. aureus methicillin-resistant S. aureus. Medicine (General) R5-920 Fan Y Pauer AC Gonzales AA Fenniri H Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes
description	Yiwen Fan1, Alexander C Pauer1, Arthur A Gonzales1, Hicham Fenniri1–3 1Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; 2Department of Bioengineering, Northeastern University, Boston, MA 02115, USA; 3Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USACorrespondence: Hicham FenniriDepartment of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USATel +1 617 373 7690Fax +1 617 373 2209Email h.fenniri@northeastern.eduPurpose: This work presents the preparation of a nanocomposite of ampicillin-conjugated gold nanoparticles (AuNPs) and self-assembled rosette nanotubes (RNTs), and evaluates its antibacterial properties against two strains of drug-resistant bacteria (Staphylococcus aureus [S. aureus], methicillin-resistant S. aureus [MRSA]).Materials and methods: Small, nearly monodisperse AuNPs (1.43±0.5 nm in diameter) nucleated on the surface of polyethylene glycol-functionalized RNTs in a one-pot reaction. Upon conjugation with ampicillin, their diameter increased to 1.86±0.32 nm. The antibacterial activity of the nanocomposite against S. aureus and MRSA was tested using different concentrations of ampicillin. The cytocompatibility of the nanocomposite was also tested against human dermal fibroblasts.Results: Based on bacterial inhibition studies, the nanocomposite demonstrated enhanced antibiotic activity against both bacterial strains. The minimum inhibitory concentration (MIC) of the nanocomposite against S. aureus was found to be 0.58 μg/mL, which was 18% lower than ampicillin alone. The nanocomposite also exhibited a 20 hrs MIC of 4 μg/mL against MRSA, approximately 10–20 times lower than previously reported values for ampicillin alone. In addition, at concentrations of 4 μg/mL of ampicillin (70 μg/mL of AuNPs), the nanocomposite showed negligible cytotoxic effects.Conclusion: Our findings offer a new approach for the treatment of drug-resistant bacteria by potentiating inhibitory effects of existing antibiotics, and delivering them using a non-toxic formulation.Keywords: ampicillin, rosette nanotubes, gold nanoparticles, S. aureus, methicillin-resistant S. aureus
format	article
author	Fan Y Pauer AC Gonzales AA Fenniri H
author_facet	Fan Y Pauer AC Gonzales AA Fenniri H
author_sort	Fan Y
title	Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes
title_short	Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes
title_full	Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes
title_fullStr	Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes
title_full_unstemmed	Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes
title_sort	enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on pegylated rosette nanotubes
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/64a852fc426541e8b755ef6874be537e
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Enhanced antibiotic activity of ampicillin conjugated to gold nanoparticles on PEGylated rosette nanotubes

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