R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)

R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)

The MYB transcription factor (TF) is crucial for plant growth, development, and response to abiotic stress, but it is rarely reported in the herbaceous peony (<i>Paeonia lactiflora</i> Pall.). Here, an MYB TF gene was isolated, and based on our prior mRNA data from <i>P. lactiflora...

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Autores principales: Yanqing Wu, Tingting Li, Zhuoya Cheng, Daqiu Zhao, Jun Tao
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
MYB
drought stress
flavonoid
ROS
<i>Paeonia lactiflora</i>
Biology (General)
QH301-705.5
Chemistry
QD1-999
Acceso en línea:https://doaj.org/article/64acf36e08be4c4e88fb7fe445e50998
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spelling oai:doaj.org-article:64acf36e08be4c4e88fb7fe445e509982021-11-11T17:18:23ZR2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)10.3390/ijms2221118841422-00671661-6596https://doaj.org/article/64acf36e08be4c4e88fb7fe445e509982021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11884https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The MYB transcription factor (TF) is crucial for plant growth, development, and response to abiotic stress, but it is rarely reported in the herbaceous peony (<i>Paeonia lactiflora</i> Pall.). Here, an MYB TF gene was isolated, and based on our prior mRNA data from <i>P. lactiflora</i> samples, it was treated with drought stress (DS). Its complete cDNA structure was 1314 bp, which encoded 291 amino acids (aa). Furthermore, using sequence alignment analysis, we demonstrated that <i>PlMYB108</i> was an R2R3-MYB TF. We also revealed that PlMYB108 was primarily localized in the nucleus. Its levels rose during DS, and it was positively correlated with drought tolerance (DT) in <i>P. lactiflora</i>. In addition, when <i>PlMYB108</i> was overexpressed in tobacco plants, the flavonoid content, antioxidant enzyme activities, and photosynthesis were markedly elevated. Hence, the transgenic plants had stronger DT with a higher leaf water content and lower H<sub>2</sub>O<sub>2</sub> accumulation compared to the wild-type (WT) plants. Based on these results, <i>PlMYB108</i> is a vital gene that serves to increase flavonoid accumulation, reactive oxygen species (ROS), scavenging capacity, and photosynthesis to confer DT. The results would provide a genetic resource for molecular breeding to enhance plant DT.Yanqing WuTingting LiZhuoya ChengDaqiu ZhaoJun TaoMDPI AGarticleMYBdrought stressflavonoidROS<i>Paeonia lactiflora</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11884, p 11884 (2021)
institution DOAJ
collection DOAJ
language EN
topic MYB
drought stress
flavonoid
ROS
<i>Paeonia lactiflora</i>
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle MYB
drought stress
flavonoid
ROS
<i>Paeonia lactiflora</i>
Biology (General)
QH301-705.5
Chemistry
QD1-999
Yanqing Wu
Tingting Li
Zhuoya Cheng
Daqiu Zhao
Jun Tao
R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)
description The MYB transcription factor (TF) is crucial for plant growth, development, and response to abiotic stress, but it is rarely reported in the herbaceous peony (<i>Paeonia lactiflora</i> Pall.). Here, an MYB TF gene was isolated, and based on our prior mRNA data from <i>P. lactiflora</i> samples, it was treated with drought stress (DS). Its complete cDNA structure was 1314 bp, which encoded 291 amino acids (aa). Furthermore, using sequence alignment analysis, we demonstrated that <i>PlMYB108</i> was an R2R3-MYB TF. We also revealed that PlMYB108 was primarily localized in the nucleus. Its levels rose during DS, and it was positively correlated with drought tolerance (DT) in <i>P. lactiflora</i>. In addition, when <i>PlMYB108</i> was overexpressed in tobacco plants, the flavonoid content, antioxidant enzyme activities, and photosynthesis were markedly elevated. Hence, the transgenic plants had stronger DT with a higher leaf water content and lower H<sub>2</sub>O<sub>2</sub> accumulation compared to the wild-type (WT) plants. Based on these results, <i>PlMYB108</i> is a vital gene that serves to increase flavonoid accumulation, reactive oxygen species (ROS), scavenging capacity, and photosynthesis to confer DT. The results would provide a genetic resource for molecular breeding to enhance plant DT.
format article
author Yanqing Wu
Tingting Li
Zhuoya Cheng
Daqiu Zhao
Jun Tao
author_facet Yanqing Wu
Tingting Li
Zhuoya Cheng
Daqiu Zhao
Jun Tao
author_sort Yanqing Wu
title R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)
title_short R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)
title_full R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)
title_fullStr R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)
title_full_unstemmed R2R3-MYB Transcription Factor <i>PlMYB108</i> Confers Drought Tolerance in Herbaceous Peony (<i>Paeonia lactiflora</i> Pall.)
title_sort r2r3-myb transcription factor <i>plmyb108</i> confers drought tolerance in herbaceous peony (<i>paeonia lactiflora</i> pall.)
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/64acf36e08be4c4e88fb7fe445e50998
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AT tingtingli r2r3mybtranscriptionfactoriplmyb108iconfersdroughttoleranceinherbaceouspeonyipaeonialactifloraipall
AT zhuoyacheng r2r3mybtranscriptionfactoriplmyb108iconfersdroughttoleranceinherbaceouspeonyipaeonialactifloraipall
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AT juntao r2r3mybtranscriptionfactoriplmyb108iconfersdroughttoleranceinherbaceouspeonyipaeonialactifloraipall
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