Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration

Abstract Exogenous application of human epidermal growth factor (hEGF) stimulates epidermal wound healing. The aim of this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin...

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Autores principales:	Teodora Bavaro, Sara Tengattini, Refaya Rezwan, Enrica Chiesa, Caterina Temporini, Rossella Dorati, Gabriella Massolini, Bice Conti, Daniela Ubiali, Marco Terreni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64b432ffe72c459892376f2d7e08c0ad
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spelling	oai:doaj.org-article:64b432ffe72c459892376f2d7e08c0ad2021-12-02T14:16:07ZDesign of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration10.1038/s41598-021-81905-12045-2322https://doaj.org/article/64b432ffe72c459892376f2d7e08c0ad2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81905-1https://doaj.org/toc/2045-2322Abstract Exogenous application of human epidermal growth factor (hEGF) stimulates epidermal wound healing. The aim of this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin-related disorders as burns. Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. EGF was immobilized on these carriers and immobilization yield was evaluated (100% and 12%, respectively). A peptide mapping of unbound protein regions was carried out by LC–MS to take evidence of the residues involved in the immobilization and, consequently, the flexibility and surface accessibility of immobilized EGF. To assess cell proliferative activities, 10, 25, 50, and 100 ng/mL of each immobilized EGF sample were seeded on fibroblast cells and incubated for 24, 48 and 72 h. The immobilized growth factor showed significantly high cell proliferative activity at 50 and 100 ng/mL compared to control and soluble EGF. Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF.Teodora BavaroSara TengattiniRefaya RezwanEnrica ChiesaCaterina TemporiniRossella DoratiGabriella MassoliniBice ContiDaniela UbialiMarco TerreniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teodora Bavaro Sara Tengattini Refaya Rezwan Enrica Chiesa Caterina Temporini Rossella Dorati Gabriella Massolini Bice Conti Daniela Ubiali Marco Terreni Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration
description	Abstract Exogenous application of human epidermal growth factor (hEGF) stimulates epidermal wound healing. The aim of this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin-related disorders as burns. Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. EGF was immobilized on these carriers and immobilization yield was evaluated (100% and 12%, respectively). A peptide mapping of unbound protein regions was carried out by LC–MS to take evidence of the residues involved in the immobilization and, consequently, the flexibility and surface accessibility of immobilized EGF. To assess cell proliferative activities, 10, 25, 50, and 100 ng/mL of each immobilized EGF sample were seeded on fibroblast cells and incubated for 24, 48 and 72 h. The immobilized growth factor showed significantly high cell proliferative activity at 50 and 100 ng/mL compared to control and soluble EGF. Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF.
format	article
author	Teodora Bavaro Sara Tengattini Refaya Rezwan Enrica Chiesa Caterina Temporini Rossella Dorati Gabriella Massolini Bice Conti Daniela Ubiali Marco Terreni
author_facet	Teodora Bavaro Sara Tengattini Refaya Rezwan Enrica Chiesa Caterina Temporini Rossella Dorati Gabriella Massolini Bice Conti Daniela Ubiali Marco Terreni
author_sort	Teodora Bavaro
title	Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration
title_short	Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration
title_full	Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration
title_fullStr	Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration
title_full_unstemmed	Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration
title_sort	design of epidermal growth factor immobilization on 3d biocompatible scaffolds to promote tissue repair and regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/64b432ffe72c459892376f2d7e08c0ad
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Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration

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