The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood
Recent advances suggest that miniaturised mid-infrared (MIR) devices could replace more time-consuming, laboratory-based techniques for clinical diagnostics. This work uses Fourier transform infrared spectroscopy to show that the MIR complex refractive index of whole blood varies across a range of h...
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oai:doaj.org-article:64b81c3955a44005ac02f089721156ec2021-11-25T16:54:57ZThe Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood10.3390/bios111104172079-6374https://doaj.org/article/64b81c3955a44005ac02f089721156ec2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/417https://doaj.org/toc/2079-6374Recent advances suggest that miniaturised mid-infrared (MIR) devices could replace more time-consuming, laboratory-based techniques for clinical diagnostics. This work uses Fourier transform infrared spectroscopy to show that the MIR complex refractive index of whole blood varies across a range of haematocrit. This indicates that the use of an evanescent measurement is not sufficient to optically exclude the cellular content of blood in the MIR, as previously assumed. Here, spectral refractive index data is presented in two ways. First, it is given as whole blood with varying haematocrit. Second, it is given as the percentage error that haematocrit introduces to plasma. The maximum error in the effective plasma refractive index due to the haematocrit of healthy adults was 0.25% for the real part n and 11% for the imaginary part k. This implies that calibration measurements of haematocrit can be used to account for errors introduced by the cellular content, enabling plasma spectra and analyte concentrations to be indirectly calculated from a whole blood sample. This methodological advance is of clinical importance as plasma concentration of analytes such as drugs can be determined using MIR without the preprocessing of whole blood.David J. RoweDaniel R. OwensSuzanne L. ParkerSaul N. FaustJames S. WilkinsonGoran Z. MashanovichMDPI AGarticlemid-infrared spectroscopybiofluid analysisbioanalytical validationpoint-of-care sensingBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 417, p 417 (2021) |
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mid-infrared spectroscopy biofluid analysis bioanalytical validation point-of-care sensing Biotechnology TP248.13-248.65 |
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mid-infrared spectroscopy biofluid analysis bioanalytical validation point-of-care sensing Biotechnology TP248.13-248.65 David J. Rowe Daniel R. Owens Suzanne L. Parker Saul N. Faust James S. Wilkinson Goran Z. Mashanovich The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood |
| description |
Recent advances suggest that miniaturised mid-infrared (MIR) devices could replace more time-consuming, laboratory-based techniques for clinical diagnostics. This work uses Fourier transform infrared spectroscopy to show that the MIR complex refractive index of whole blood varies across a range of haematocrit. This indicates that the use of an evanescent measurement is not sufficient to optically exclude the cellular content of blood in the MIR, as previously assumed. Here, spectral refractive index data is presented in two ways. First, it is given as whole blood with varying haematocrit. Second, it is given as the percentage error that haematocrit introduces to plasma. The maximum error in the effective plasma refractive index due to the haematocrit of healthy adults was 0.25% for the real part n and 11% for the imaginary part k. This implies that calibration measurements of haematocrit can be used to account for errors introduced by the cellular content, enabling plasma spectra and analyte concentrations to be indirectly calculated from a whole blood sample. This methodological advance is of clinical importance as plasma concentration of analytes such as drugs can be determined using MIR without the preprocessing of whole blood. |
| format |
article |
| author |
David J. Rowe Daniel R. Owens Suzanne L. Parker Saul N. Faust James S. Wilkinson Goran Z. Mashanovich |
| author_facet |
David J. Rowe Daniel R. Owens Suzanne L. Parker Saul N. Faust James S. Wilkinson Goran Z. Mashanovich |
| author_sort |
David J. Rowe |
| title |
The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood |
| title_short |
The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood |
| title_full |
The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood |
| title_fullStr |
The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood |
| title_full_unstemmed |
The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood |
| title_sort |
effect of haematocrit on measurement of the mid-infrared refractive index of plasma in whole blood |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/64b81c3955a44005ac02f089721156ec |
| work_keys_str_mv |
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