Characterization of prophages of Lactococcus garvieae

Abstract This report describes the morphological characterization and genome analysis of an induced prophage (PLg-TB25) from a dairy strain of Lactococcus garvieae. The phage belongs to the Siphoviridae family and its morphology is typical of other lactococcal phages. A general analysis of its genom...

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Autores principales:	Giovanni Eraclio, Maria G. Fortina, Simon J. Labrie, Denise M. Tremblay, Sylvain Moineau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64ba02795c2c4bee93af8f108b47eb5c
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spelling	oai:doaj.org-article:64ba02795c2c4bee93af8f108b47eb5c2021-12-02T16:08:09ZCharacterization of prophages of Lactococcus garvieae10.1038/s41598-017-02038-y2045-2322https://doaj.org/article/64ba02795c2c4bee93af8f108b47eb5c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02038-yhttps://doaj.org/toc/2045-2322Abstract This report describes the morphological characterization and genome analysis of an induced prophage (PLg-TB25) from a dairy strain of Lactococcus garvieae. The phage belongs to the Siphoviridae family and its morphology is typical of other lactococcal phages. A general analysis of its genome did not reveal similarities with other lactococcal phage genomes, confirming its novelty. However, similarities were found between genes of its morphogenesis cluster and genes of Gram-positive bacteria, suggesting that this phage genome resulted from recombination events that took place in a heterogeneous microbial environment. An in silico search for other prophages in 16 L. garvieae genomes available in public databases, uncovered eight seemingly complete prophages in strains isolated from dairy and fish niches. Genome analyses of these prophages revealed three novel L. garvieae phages. The remaining prophages had homology to phages of Lactococcus lactis (P335 group) suggesting a close relationship between these lactococcal species. The similarity in GC content of L. garvieae prophages to the genomes of L. lactis phages further supports the hypothesis that these phages likely originated from the same ancestor.Giovanni EraclioMaria G. FortinaSimon J. LabrieDenise M. TremblaySylvain MoineauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giovanni Eraclio Maria G. Fortina Simon J. Labrie Denise M. Tremblay Sylvain Moineau Characterization of prophages of Lactococcus garvieae
description	Abstract This report describes the morphological characterization and genome analysis of an induced prophage (PLg-TB25) from a dairy strain of Lactococcus garvieae. The phage belongs to the Siphoviridae family and its morphology is typical of other lactococcal phages. A general analysis of its genome did not reveal similarities with other lactococcal phage genomes, confirming its novelty. However, similarities were found between genes of its morphogenesis cluster and genes of Gram-positive bacteria, suggesting that this phage genome resulted from recombination events that took place in a heterogeneous microbial environment. An in silico search for other prophages in 16 L. garvieae genomes available in public databases, uncovered eight seemingly complete prophages in strains isolated from dairy and fish niches. Genome analyses of these prophages revealed three novel L. garvieae phages. The remaining prophages had homology to phages of Lactococcus lactis (P335 group) suggesting a close relationship between these lactococcal species. The similarity in GC content of L. garvieae prophages to the genomes of L. lactis phages further supports the hypothesis that these phages likely originated from the same ancestor.
format	article
author	Giovanni Eraclio Maria G. Fortina Simon J. Labrie Denise M. Tremblay Sylvain Moineau
author_facet	Giovanni Eraclio Maria G. Fortina Simon J. Labrie Denise M. Tremblay Sylvain Moineau
author_sort	Giovanni Eraclio
title	Characterization of prophages of Lactococcus garvieae
title_short	Characterization of prophages of Lactococcus garvieae
title_full	Characterization of prophages of Lactococcus garvieae
title_fullStr	Characterization of prophages of Lactococcus garvieae
title_full_unstemmed	Characterization of prophages of Lactococcus garvieae
title_sort	characterization of prophages of lactococcus garvieae
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/64ba02795c2c4bee93af8f108b47eb5c
work_keys_str_mv	AT giovannieraclio characterizationofprophagesoflactococcusgarvieae AT mariagfortina characterizationofprophagesoflactococcusgarvieae AT simonjlabrie characterizationofprophagesoflactococcusgarvieae AT denisemtremblay characterizationofprophagesoflactococcusgarvieae AT sylvainmoineau characterizationofprophagesoflactococcusgarvieae
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Characterization of prophages of Lactococcus garvieae

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