Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.

Neutrophils (PMNs) and cytokines have a critical role to play in host defense and systemic inflammatory response syndrome (SIRS). Neutrophil extracellular traps (NETs) have been shown to extracellularly kill pathogens, and inflammatory potential of NETs has been shown. Microbial killing inside the p...

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Autores principales:	Ravi S Keshari, Anupam Jyoti, Megha Dubey, Nikhil Kothari, Monica Kohli, Jaishri Bogra, Manoj K Barthwal, Madhu Dikshit
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64ba3da3a044425c93cf5f32187059ca
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spelling	oai:doaj.org-article:64ba3da3a044425c93cf5f32187059ca2021-11-18T08:10:52ZCytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.1932-620310.1371/journal.pone.0048111https://doaj.org/article/64ba3da3a044425c93cf5f32187059ca2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110185/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Neutrophils (PMNs) and cytokines have a critical role to play in host defense and systemic inflammatory response syndrome (SIRS). Neutrophil extracellular traps (NETs) have been shown to extracellularly kill pathogens, and inflammatory potential of NETs has been shown. Microbial killing inside the phagosomes or by NETs is mediated by reactive oxygen and nitrogen species (ROS/RNS). The present study was undertaken to assess circulating NETs contents and frequency of NETs generation by isolated PMNs from SIRS patients. These patients displayed significant augmentation in the circulating myeloperoxidase (MPO) activity and DNA content, while PMA stimulated PMNs from these patients, generated more free radicals and NETs. Plasma obtained from SIRS patients, if added to the PMNs isolated from healthy subjects, enhanced NETs release and free radical formation. Expressions of inflammatory cytokines (IL-1β, TNFα and IL-8) in the PMNs as well as their circulating levels were significantly augmented in SIRS subjects. Treatment of neutrophils from healthy subjects with TNFα, IL-1β, or IL-8 enhanced free radicals generation and NETs formation, which was mediated through the activation of NADPH oxidase and MPO. Pre-incubation of plasma from SIRS with TNFα, IL-1β, or IL-8 antibodies reduced the NETs release. Role of IL-1β, TNFα and IL-8 thus seems to be involved in the enhanced release of NETs in SIRS subjects.Ravi S KeshariAnupam JyotiMegha DubeyNikhil KothariMonica KohliJaishri BograManoj K BarthwalMadhu DikshitPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48111 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ravi S Keshari Anupam Jyoti Megha Dubey Nikhil Kothari Monica Kohli Jaishri Bogra Manoj K Barthwal Madhu Dikshit Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.
description	Neutrophils (PMNs) and cytokines have a critical role to play in host defense and systemic inflammatory response syndrome (SIRS). Neutrophil extracellular traps (NETs) have been shown to extracellularly kill pathogens, and inflammatory potential of NETs has been shown. Microbial killing inside the phagosomes or by NETs is mediated by reactive oxygen and nitrogen species (ROS/RNS). The present study was undertaken to assess circulating NETs contents and frequency of NETs generation by isolated PMNs from SIRS patients. These patients displayed significant augmentation in the circulating myeloperoxidase (MPO) activity and DNA content, while PMA stimulated PMNs from these patients, generated more free radicals and NETs. Plasma obtained from SIRS patients, if added to the PMNs isolated from healthy subjects, enhanced NETs release and free radical formation. Expressions of inflammatory cytokines (IL-1β, TNFα and IL-8) in the PMNs as well as their circulating levels were significantly augmented in SIRS subjects. Treatment of neutrophils from healthy subjects with TNFα, IL-1β, or IL-8 enhanced free radicals generation and NETs formation, which was mediated through the activation of NADPH oxidase and MPO. Pre-incubation of plasma from SIRS with TNFα, IL-1β, or IL-8 antibodies reduced the NETs release. Role of IL-1β, TNFα and IL-8 thus seems to be involved in the enhanced release of NETs in SIRS subjects.
format	article
author	Ravi S Keshari Anupam Jyoti Megha Dubey Nikhil Kothari Monica Kohli Jaishri Bogra Manoj K Barthwal Madhu Dikshit
author_facet	Ravi S Keshari Anupam Jyoti Megha Dubey Nikhil Kothari Monica Kohli Jaishri Bogra Manoj K Barthwal Madhu Dikshit
author_sort	Ravi S Keshari
title	Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.
title_short	Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.
title_full	Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.
title_fullStr	Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.
title_full_unstemmed	Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.
title_sort	cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/64ba3da3a044425c93cf5f32187059ca
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Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition.

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