High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)

Arracacha is a root crop cultivated in several countries of South America. The commercial product, which is the storage root, has multiple nutritional properties: its fine starch makes it easily digestible, and it contains high levels of calcium and vitamin A. High throughput sequencing (HTS) techno...

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Autores principales:	Joao De Souza, Segundo Fuentes, Giovanna Müller, Heidy Gamarra, Mónica Guzmán, Wilmer Cuellar, Jan Kreuze
Formato:	article
Lenguaje:	EN ES PT
Publicado:	Universidad Nacional de Trujillo 2021
Materias:	arracacha sirna sequencing crinivirus vitivirus enamovirus Agriculture S Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/64bc717e11064f0f8622b7d29c6b785f
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spelling	oai:doaj.org-article:64bc717e11064f0f8622b7d29c6b785f2021-11-04T14:33:12ZHigh throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)10.17268/sci.agropecu.2021.0512077-99172306-6741https://doaj.org/article/64bc717e11064f0f8622b7d29c6b785f2021-12-01T00:00:00Zhttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/3927https://doaj.org/toc/2077-9917https://doaj.org/toc/2306-6741Arracacha is a root crop cultivated in several countries of South America. The commercial product, which is the storage root, has multiple nutritional properties: its fine starch makes it easily digestible, and it contains high levels of calcium and vitamin A. High throughput sequencing (HTS) technology has been applied successfully for virus discovery in different agricultural crops, and it has been proposed to apply it in routine pathogen detection. Using HTS, novel sequences related to crinivirus and vitivirus were identified in apparently symptomeless arracacha, which were assembled into contigs located in different positions of the genome. Based on those sequences’ primers were designed to amplify corresponding sequences from further arracacha accessions and potato plant samples collected from farmers’ fields in Colombia and Peru. It was possible to determine the near complete genomes of these viruses. In addition, sequences related to an enamovirus and ST9-like RNA were also identified in arracacha plants using HTS.Joao De SouzaSegundo FuentesGiovanna MüllerHeidy GamarraMónica GuzmánWilmer CuellarJan KreuzeUniversidad Nacional de Trujilloarticlearracachasirna sequencingcrinivirusvitivirusenamovirusAgricultureSAgriculture (General)S1-972ENESPTScientia Agropecuaria, Vol 12, Iss 4, Pp 471-480 (2021)
institution	DOAJ
collection	DOAJ
language	EN ES PT
topic	arracacha sirna sequencing crinivirus vitivirus enamovirus Agriculture S Agriculture (General) S1-972
spellingShingle	arracacha sirna sequencing crinivirus vitivirus enamovirus Agriculture S Agriculture (General) S1-972 Joao De Souza Segundo Fuentes Giovanna Müller Heidy Gamarra Mónica Guzmán Wilmer Cuellar Jan Kreuze High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)
description	Arracacha is a root crop cultivated in several countries of South America. The commercial product, which is the storage root, has multiple nutritional properties: its fine starch makes it easily digestible, and it contains high levels of calcium and vitamin A. High throughput sequencing (HTS) technology has been applied successfully for virus discovery in different agricultural crops, and it has been proposed to apply it in routine pathogen detection. Using HTS, novel sequences related to crinivirus and vitivirus were identified in apparently symptomeless arracacha, which were assembled into contigs located in different positions of the genome. Based on those sequences’ primers were designed to amplify corresponding sequences from further arracacha accessions and potato plant samples collected from farmers’ fields in Colombia and Peru. It was possible to determine the near complete genomes of these viruses. In addition, sequences related to an enamovirus and ST9-like RNA were also identified in arracacha plants using HTS.
format	article
author	Joao De Souza Segundo Fuentes Giovanna Müller Heidy Gamarra Mónica Guzmán Wilmer Cuellar Jan Kreuze
author_facet	Joao De Souza Segundo Fuentes Giovanna Müller Heidy Gamarra Mónica Guzmán Wilmer Cuellar Jan Kreuze
author_sort	Joao De Souza
title	High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)
title_short	High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)
title_full	High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)
title_fullStr	High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)
title_full_unstemmed	High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)
title_sort	high throughput sequencing for the detection and characterization of new virus found in arracacha (arracacia xanthorrhiza)
publisher	Universidad Nacional de Trujillo
publishDate	2021
url	https://doaj.org/article/64bc717e11064f0f8622b7d29c6b785f
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High throughput sequencing for the detection and characterization of new virus found in arracacha (Arracacia xanthorrhiza)

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