Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice
Abstract Active wound dressings are attracting extensive attention in soft tissue repair and regeneration, including bacteria-infected skin wound healing. As the wide use of antibiotics leads to drug resistance we present here a new concept of wound dressings based on the polycaprolactone nanofiber...
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Nature Portfolio
2021
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oai:doaj.org-article:64cdaee56ed840f5a816a7b0b783e2122021-12-02T19:04:19ZNovel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice10.1038/s41598-021-96980-72045-2322https://doaj.org/article/64cdaee56ed840f5a816a7b0b783e2122021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96980-7https://doaj.org/toc/2045-2322Abstract Active wound dressings are attracting extensive attention in soft tissue repair and regeneration, including bacteria-infected skin wound healing. As the wide use of antibiotics leads to drug resistance we present here a new concept of wound dressings based on the polycaprolactone nanofiber scaffold (NANO) releasing second generation lipophosphonoxin (LPPO) as antibacterial agent. Firstly, we demonstrated in vitro that LPPO released from NANO exerted antibacterial activity while not impairing proliferation/differentiation of fibroblasts and keratinocytes. Secondly, using a mouse model we showed that NANO loaded with LPPO significantly reduced the Staphylococcus aureus counts in infected wounds as evaluated 7 days post-surgery. Furthermore, the rate of degradation and subsequent LPPO release in infected wounds was also facilitated by lytic enzymes secreted by inoculated bacteria. Finally, LPPO displayed negligible to no systemic absorption. In conclusion, the composite antibacterial NANO-LPPO-based dressing reduces the bacterial load and promotes skin repair, with the potential to treat wounds in clinical settings.Duy Dinh Do PhamVěra JenčováMiriam KaňuchováJan BayramIvana GrossováHubert ŠucaLukáš UrbanKristýna HavlíčkováVít NovotnýPetr MikešViktor MojrNikifor AsatianiEva Kuželová KošťákováMartina MaixnerováAlena VlkováDragana VítovskáHana ŠanderováAlexandr NemecLibor KrásnýRobert ZajíčekDavid LukášDominik RejmanPeter GálNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
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Medicine R Science Q Duy Dinh Do Pham Věra Jenčová Miriam Kaňuchová Jan Bayram Ivana Grossová Hubert Šuca Lukáš Urban Kristýna Havlíčková Vít Novotný Petr Mikeš Viktor Mojr Nikifor Asatiani Eva Kuželová Košťáková Martina Maixnerová Alena Vlková Dragana Vítovská Hana Šanderová Alexandr Nemec Libor Krásný Robert Zajíček David Lukáš Dominik Rejman Peter Gál Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice |
| description |
Abstract Active wound dressings are attracting extensive attention in soft tissue repair and regeneration, including bacteria-infected skin wound healing. As the wide use of antibiotics leads to drug resistance we present here a new concept of wound dressings based on the polycaprolactone nanofiber scaffold (NANO) releasing second generation lipophosphonoxin (LPPO) as antibacterial agent. Firstly, we demonstrated in vitro that LPPO released from NANO exerted antibacterial activity while not impairing proliferation/differentiation of fibroblasts and keratinocytes. Secondly, using a mouse model we showed that NANO loaded with LPPO significantly reduced the Staphylococcus aureus counts in infected wounds as evaluated 7 days post-surgery. Furthermore, the rate of degradation and subsequent LPPO release in infected wounds was also facilitated by lytic enzymes secreted by inoculated bacteria. Finally, LPPO displayed negligible to no systemic absorption. In conclusion, the composite antibacterial NANO-LPPO-based dressing reduces the bacterial load and promotes skin repair, with the potential to treat wounds in clinical settings. |
| format |
article |
| author |
Duy Dinh Do Pham Věra Jenčová Miriam Kaňuchová Jan Bayram Ivana Grossová Hubert Šuca Lukáš Urban Kristýna Havlíčková Vít Novotný Petr Mikeš Viktor Mojr Nikifor Asatiani Eva Kuželová Košťáková Martina Maixnerová Alena Vlková Dragana Vítovská Hana Šanderová Alexandr Nemec Libor Krásný Robert Zajíček David Lukáš Dominik Rejman Peter Gál |
| author_facet |
Duy Dinh Do Pham Věra Jenčová Miriam Kaňuchová Jan Bayram Ivana Grossová Hubert Šuca Lukáš Urban Kristýna Havlíčková Vít Novotný Petr Mikeš Viktor Mojr Nikifor Asatiani Eva Kuželová Košťáková Martina Maixnerová Alena Vlková Dragana Vítovská Hana Šanderová Alexandr Nemec Libor Krásný Robert Zajíček David Lukáš Dominik Rejman Peter Gál |
| author_sort |
Duy Dinh Do Pham |
| title |
Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice |
| title_short |
Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice |
| title_full |
Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice |
| title_fullStr |
Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice |
| title_full_unstemmed |
Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice |
| title_sort |
novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces staphylococcus aureus induced wound infection in mice |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/64cdaee56ed840f5a816a7b0b783e212 |
| work_keys_str_mv |
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