Stability of Circulating Exosomal miRNAs in Healthy Subjects

Abstract Exosomes are nano-vesicles present in the circulation that are involved in cell-to-cell communication and regulation of different biological processes. MicroRNAs (miRNAs) are part of their cargo and are potential biomarkers. Methods of exosome isolation and the inter-individual and intra-in...

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Autores principales:	David Sanz-Rubio, Inmaculada Martin-Burriel, Ana Gil, Pablo Cubero, Marta Forner, Abdelnaby Khalyfa, Jose M. Marin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64d06e731dc849caa3aad20f369fb54f
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spelling	oai:doaj.org-article:64d06e731dc849caa3aad20f369fb54f2021-12-02T15:07:49ZStability of Circulating Exosomal miRNAs in Healthy Subjects10.1038/s41598-018-28748-52045-2322https://doaj.org/article/64d06e731dc849caa3aad20f369fb54f2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28748-5https://doaj.org/toc/2045-2322Abstract Exosomes are nano-vesicles present in the circulation that are involved in cell-to-cell communication and regulation of different biological processes. MicroRNAs (miRNAs) are part of their cargo and are potential biomarkers. Methods of exosome isolation and the inter-individual and intra-individual variations in circulating miRNA exosomal cargo have been poorly investigated. This study aims for comparing two exosome isolation methods and to assess the stability of eleven plasma exosomal miRNAs over time. In addition to evaluate miRNA variability of both kits, the effect of freezing plasma before exosome isolation or freezing isolated exosomes on miRNA stability was also evaluated. MiRNA levels were tested in 7 healthy subjects who underwent four different blood extractions obtained in 4 consecutive weeks. One of the isolation kits displayed generally better amplification signals, and miRNAs from exosomes isolated after freezing the plasma had the highest levels. Intra-subject and inter-subject coefficients of variance were lower for the same isolation kit after freezing plasma. Finally, miRNAs that showed an acceptable expression level were stable across the consecutive extractions. This study shows for the first time the stability over time of miRNAs isolated from circulating plasma exosomes, establishing a key step in the use of exosomal miRNAs as biomarkers.David Sanz-RubioInmaculada Martin-BurrielAna GilPablo CuberoMarta FornerAbdelnaby KhalyfaJose M. MarinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Sanz-Rubio Inmaculada Martin-Burriel Ana Gil Pablo Cubero Marta Forner Abdelnaby Khalyfa Jose M. Marin Stability of Circulating Exosomal miRNAs in Healthy Subjects
description	Abstract Exosomes are nano-vesicles present in the circulation that are involved in cell-to-cell communication and regulation of different biological processes. MicroRNAs (miRNAs) are part of their cargo and are potential biomarkers. Methods of exosome isolation and the inter-individual and intra-individual variations in circulating miRNA exosomal cargo have been poorly investigated. This study aims for comparing two exosome isolation methods and to assess the stability of eleven plasma exosomal miRNAs over time. In addition to evaluate miRNA variability of both kits, the effect of freezing plasma before exosome isolation or freezing isolated exosomes on miRNA stability was also evaluated. MiRNA levels were tested in 7 healthy subjects who underwent four different blood extractions obtained in 4 consecutive weeks. One of the isolation kits displayed generally better amplification signals, and miRNAs from exosomes isolated after freezing the plasma had the highest levels. Intra-subject and inter-subject coefficients of variance were lower for the same isolation kit after freezing plasma. Finally, miRNAs that showed an acceptable expression level were stable across the consecutive extractions. This study shows for the first time the stability over time of miRNAs isolated from circulating plasma exosomes, establishing a key step in the use of exosomal miRNAs as biomarkers.
format	article
author	David Sanz-Rubio Inmaculada Martin-Burriel Ana Gil Pablo Cubero Marta Forner Abdelnaby Khalyfa Jose M. Marin
author_facet	David Sanz-Rubio Inmaculada Martin-Burriel Ana Gil Pablo Cubero Marta Forner Abdelnaby Khalyfa Jose M. Marin
author_sort	David Sanz-Rubio
title	Stability of Circulating Exosomal miRNAs in Healthy Subjects
title_short	Stability of Circulating Exosomal miRNAs in Healthy Subjects
title_full	Stability of Circulating Exosomal miRNAs in Healthy Subjects
title_fullStr	Stability of Circulating Exosomal miRNAs in Healthy Subjects
title_full_unstemmed	Stability of Circulating Exosomal miRNAs in Healthy Subjects
title_sort	stability of circulating exosomal mirnas in healthy subjects
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/64d06e731dc849caa3aad20f369fb54f
work_keys_str_mv	AT davidsanzrubio stabilityofcirculatingexosomalmirnasinhealthysubjects AT inmaculadamartinburriel stabilityofcirculatingexosomalmirnasinhealthysubjects AT anagil stabilityofcirculatingexosomalmirnasinhealthysubjects AT pablocubero stabilityofcirculatingexosomalmirnasinhealthysubjects AT martaforner stabilityofcirculatingexosomalmirnasinhealthysubjects AT abdelnabykhalyfa stabilityofcirculatingexosomalmirnasinhealthysubjects AT josemmarin stabilityofcirculatingexosomalmirnasinhealthysubjects
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Stability of Circulating Exosomal miRNAs in Healthy Subjects

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