Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota

Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explant...

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Autores principales:	D P Prakash, B S Deepali, R Asokan, Y L Ramachandra, Lalitha Anand, Vageeshbabu S Hanur
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2007
Materias:	growth regulators explant brinjal transformation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/64d1b71397c640fdb6ba0372fa5303cd
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spelling	oai:doaj.org-article:64d1b71397c640fdb6ba0372fa5303cd2021-12-02T12:39:44ZEffects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota0973-354X2582-4899https://doaj.org/article/64d1b71397c640fdb6ba0372fa5303cd2007-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/612https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.D P PrakashB S DeepaliR AsokanY L RamachandraLalitha AnandVageeshbabu S HanurSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlegrowth regulatorsexplantbrinjaltransformationPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 2, Iss 2, Pp 94-98 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	growth regulators explant brinjal transformation Plant culture SB1-1110
spellingShingle	growth regulators explant brinjal transformation Plant culture SB1-1110 D P Prakash B S Deepali R Asokan Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
description	Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.
format	article
author	D P Prakash B S Deepali R Asokan Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur
author_facet	D P Prakash B S Deepali R Asokan Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur
author_sort	D P Prakash
title	Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
title_short	Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
title_full	Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
title_fullStr	Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
title_full_unstemmed	Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
title_sort	effects of growth regulators and explant-type on agrobacterium-mediated transformation in brinjal (solanum melongena l.) cv. manjarigota
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2007
url	https://doaj.org/article/64d1b71397c640fdb6ba0372fa5303cd
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Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota

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