The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins
Abstract Despite the pivotal role of jasmonic acid in the outcome of plant-microorganism interactions, JA-signaling components in roots of perennial trees like western balsam poplar (Populus trichocarpa) are poorly characterized. Here we decipher the poplar-root JA-perception complex centered on PtJ...
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2020
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oai:doaj.org-article:64d756e0882048ebbb1ac6802fe3c89f2021-12-02T15:10:25ZThe mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins10.1038/s41598-020-76832-62045-2322https://doaj.org/article/64d756e0882048ebbb1ac6802fe3c89f2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-76832-6https://doaj.org/toc/2045-2322Abstract Despite the pivotal role of jasmonic acid in the outcome of plant-microorganism interactions, JA-signaling components in roots of perennial trees like western balsam poplar (Populus trichocarpa) are poorly characterized. Here we decipher the poplar-root JA-perception complex centered on PtJAZ6, a co-repressor of JA-signaling targeted by the effector protein MiSSP7 from the ectomycorrhizal basidiomycete Laccaria bicolor during symbiotic development. Through protein–protein interaction studies in yeast we determined the poplar root proteins interacting with PtJAZ6. Moreover, we assessed via yeast triple-hybrid how the mutualistic effector MiSSP7 reshapes the association between PtJAZ6 and its partner proteins. In the absence of the symbiotic effector, PtJAZ6 interacts with the transcription factors PtMYC2s and PtJAM1.1. In addition, PtJAZ6 interacts with it-self and with other Populus JAZ proteins. Finally, MiSSP7 strengthens the binding of PtJAZ6 to PtMYC2.1 and antagonizes PtJAZ6 homo-/heterodimerization. We conclude that a symbiotic effector secreted by a mutualistic fungus may promote the symbiotic interaction through altered dynamics of a JA-signaling-associated protein–protein interaction network, maintaining the repression of PtMYC2.1-regulated genes.Yohann DaguerreVeronica BassoSebastian Hartmann-WittulskiRomain SchellenbergerLaura MeyerJustine BaillyAnnegret KohlerJonathan M. PlettFrancis MartinClaire Veneault-FourreyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) |
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Medicine R Science Q Yohann Daguerre Veronica Basso Sebastian Hartmann-Wittulski Romain Schellenberger Laura Meyer Justine Bailly Annegret Kohler Jonathan M. Plett Francis Martin Claire Veneault-Fourrey The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins |
| description |
Abstract Despite the pivotal role of jasmonic acid in the outcome of plant-microorganism interactions, JA-signaling components in roots of perennial trees like western balsam poplar (Populus trichocarpa) are poorly characterized. Here we decipher the poplar-root JA-perception complex centered on PtJAZ6, a co-repressor of JA-signaling targeted by the effector protein MiSSP7 from the ectomycorrhizal basidiomycete Laccaria bicolor during symbiotic development. Through protein–protein interaction studies in yeast we determined the poplar root proteins interacting with PtJAZ6. Moreover, we assessed via yeast triple-hybrid how the mutualistic effector MiSSP7 reshapes the association between PtJAZ6 and its partner proteins. In the absence of the symbiotic effector, PtJAZ6 interacts with the transcription factors PtMYC2s and PtJAM1.1. In addition, PtJAZ6 interacts with it-self and with other Populus JAZ proteins. Finally, MiSSP7 strengthens the binding of PtJAZ6 to PtMYC2.1 and antagonizes PtJAZ6 homo-/heterodimerization. We conclude that a symbiotic effector secreted by a mutualistic fungus may promote the symbiotic interaction through altered dynamics of a JA-signaling-associated protein–protein interaction network, maintaining the repression of PtMYC2.1-regulated genes. |
| format |
article |
| author |
Yohann Daguerre Veronica Basso Sebastian Hartmann-Wittulski Romain Schellenberger Laura Meyer Justine Bailly Annegret Kohler Jonathan M. Plett Francis Martin Claire Veneault-Fourrey |
| author_facet |
Yohann Daguerre Veronica Basso Sebastian Hartmann-Wittulski Romain Schellenberger Laura Meyer Justine Bailly Annegret Kohler Jonathan M. Plett Francis Martin Claire Veneault-Fourrey |
| author_sort |
Yohann Daguerre |
| title |
The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins |
| title_short |
The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins |
| title_full |
The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins |
| title_fullStr |
The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins |
| title_full_unstemmed |
The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins |
| title_sort |
mutualism effector missp7 of laccaria bicolor alters the interactions between the poplar jaz6 protein and its associated proteins |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/64d756e0882048ebbb1ac6802fe3c89f |
| work_keys_str_mv |
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