Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation

Abstract Glycosylation of uterine endometrial cells plays important roles to determine their receptive function to blastocysts. Trophoblast-derived pregnancy-associated plasma protein A (PAPPA) is specifically elevated in pregnant women serum, and is known to promote trophoblast cell proliferation a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ming Yu, Jiao Wang, Shuai Liu, Xiaoqi Wang, Qiu Yan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64d842a7b81d4459832085bb33547c09
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:64d842a7b81d4459832085bb33547c09
record_format	dspace
spelling	oai:doaj.org-article:64d842a7b81d4459832085bb33547c092021-12-02T11:53:03ZNovel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation10.1038/s41598-017-04735-02045-2322https://doaj.org/article/64d842a7b81d4459832085bb33547c092017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04735-0https://doaj.org/toc/2045-2322Abstract Glycosylation of uterine endometrial cells plays important roles to determine their receptive function to blastocysts. Trophoblast-derived pregnancy-associated plasma protein A (PAPPA) is specifically elevated in pregnant women serum, and is known to promote trophoblast cell proliferation and adhesion. However, the relationship between PAPPA and endometrium receptivity, as well as the regulation of N-fucosylation remains unclear. We found that rhPAPPA and PAPPA in the serum samples from pregnant women or conditioned medium of trophoblast cells promoted endometrium receptivity in vitro. Moreover, rhPAPPA increased α1,2-, α1,3- and α1,6-fucosylation levels by up-regulating N-fucosyltransferases FUT1, FUT4 and FUT8 expression, respectively, through IGF-1R/PI3K/Akt signaling pathway in human endometrial cells. Additionally, α1,2-, α1,3- and α1,6-fucosylation of integrin αVβ3, a critical endometrium receptivity biomarker, was up-regulated by PAPPA, thereby enhanced its adhesive functions. Furthermore, PAPPA blockage with antibody inhibited embryo implantation in vivo, mouse embryo adhesion and spreading in vitro, as well as N-fucosylation level of the endometrium in pregnant mice. In summary, this study suggests that PAPPA is essential to maintain a receptive endometrium by up-regulating N-fucosylation, which is a potential useful biomarker to evaluate the receptive functions of the endometrium.Ming YuJiao WangShuai LiuXiaoqi WangQiu YanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming Yu Jiao Wang Shuai Liu Xiaoqi Wang Qiu Yan Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation
description	Abstract Glycosylation of uterine endometrial cells plays important roles to determine their receptive function to blastocysts. Trophoblast-derived pregnancy-associated plasma protein A (PAPPA) is specifically elevated in pregnant women serum, and is known to promote trophoblast cell proliferation and adhesion. However, the relationship between PAPPA and endometrium receptivity, as well as the regulation of N-fucosylation remains unclear. We found that rhPAPPA and PAPPA in the serum samples from pregnant women or conditioned medium of trophoblast cells promoted endometrium receptivity in vitro. Moreover, rhPAPPA increased α1,2-, α1,3- and α1,6-fucosylation levels by up-regulating N-fucosyltransferases FUT1, FUT4 and FUT8 expression, respectively, through IGF-1R/PI3K/Akt signaling pathway in human endometrial cells. Additionally, α1,2-, α1,3- and α1,6-fucosylation of integrin αVβ3, a critical endometrium receptivity biomarker, was up-regulated by PAPPA, thereby enhanced its adhesive functions. Furthermore, PAPPA blockage with antibody inhibited embryo implantation in vivo, mouse embryo adhesion and spreading in vitro, as well as N-fucosylation level of the endometrium in pregnant mice. In summary, this study suggests that PAPPA is essential to maintain a receptive endometrium by up-regulating N-fucosylation, which is a potential useful biomarker to evaluate the receptive functions of the endometrium.
format	article
author	Ming Yu Jiao Wang Shuai Liu Xiaoqi Wang Qiu Yan
author_facet	Ming Yu Jiao Wang Shuai Liu Xiaoqi Wang Qiu Yan
author_sort	Ming Yu
title	Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation
title_short	Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation
title_full	Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation
title_fullStr	Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation
title_full_unstemmed	Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation
title_sort	novel function of pregnancy-associated plasma protein a: promotes endometrium receptivity by up-regulating n-fucosylation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/64d842a7b81d4459832085bb33547c09
work_keys_str_mv	AT mingyu novelfunctionofpregnancyassociatedplasmaproteinapromotesendometriumreceptivitybyupregulatingnfucosylation AT jiaowang novelfunctionofpregnancyassociatedplasmaproteinapromotesendometriumreceptivitybyupregulatingnfucosylation AT shuailiu novelfunctionofpregnancyassociatedplasmaproteinapromotesendometriumreceptivitybyupregulatingnfucosylation AT xiaoqiwang novelfunctionofpregnancyassociatedplasmaproteinapromotesendometriumreceptivitybyupregulatingnfucosylation AT qiuyan novelfunctionofpregnancyassociatedplasmaproteinapromotesendometriumreceptivitybyupregulatingnfucosylation
_version_	1718394869451325440

Novel function of pregnancy-associated plasma protein A: promotes endometrium receptivity by up-regulating N-fucosylation

Ejemplares similares