Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells

Abstract Background Stem cell-based therapies for neurodegenerative diseases like Parkinson’s disease are a promising approach in regenerative medicine and are now moving towards early stage clinical trials. However, a number of challenges remain including the ability to grow stem cells in vitro on...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Miranda Robbins, Venkat Pisupati, Roberta Azzarelli, Samer I. Nehme, Roger A. Barker, Ljiljana Fruk, Gabriele S. Kaminski Schierle
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Stem cells Neurodegeneration Biofunctionalisation Cell scaffold Implantation Tissue engineering Medicine (General) R5-920 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/64d85d1a1edc4887b5086fb9bb822a05
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:64d85d1a1edc4887b5086fb9bb822a05
record_format	dspace
spelling	oai:doaj.org-article:64d85d1a1edc4887b5086fb9bb822a052021-11-14T12:08:06ZBiofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells10.1186/s13287-021-02639-51757-6512https://doaj.org/article/64d85d1a1edc4887b5086fb9bb822a052021-11-01T00:00:00Zhttps://doi.org/10.1186/s13287-021-02639-5https://doaj.org/toc/1757-6512Abstract Background Stem cell-based therapies for neurodegenerative diseases like Parkinson’s disease are a promising approach in regenerative medicine and are now moving towards early stage clinical trials. However, a number of challenges remain including the ability to grow stem cells in vitro on a 3-dimensional scaffold, as well as their loss, by leakage or cell death, post-implantation. These issues could, however, be helped through the use of scaffolds that support the growth and differentiation of stem cells both in vitro and in vivo. The present study focuses on the use of bacterial cellulose as an in vitro scaffold to promote the growth of different stem cell-derived cell types. Bacterial cellulose was used because of its remarkable properties such as its wettability, ability to retain water and low stiffness, all of which is similar to that found in brain tissue. Methods We cultured human embryonic stem cell-derived progenitor cells on bacterial cellulose with growth factors that were covalently functionalised to the surface via silanisation. Epifluorescence microscopy and immunofluorescence were used to detect the differentiation of stem cells into dopaminergic ventral midbrain progenitor cells. We then quantified the proportion of cells that differentiated into progenitor cells and compared the effect of growing cells on biofunctionalised cellulose versus standard cellulose. Results We show that the covalent functionalisation of bacterial cellulose sheets with bioactive peptides improves the growth and differentiation of human pluripotent stem cells into dopaminergic neuronal progenitors. Conclusions This study suggests that the biocompatible material, bacterial cellulose, has potential applications in cell therapy approaches as a means to repair damage to the central nervous system, such as in Parkinson’s disease but also in tissue engineering.Miranda RobbinsVenkat PisupatiRoberta AzzarelliSamer I. NehmeRoger A. BarkerLjiljana FrukGabriele S. Kaminski SchierleBMCarticleStem cellsNeurodegenerationBiofunctionalisationCell scaffoldImplantationTissue engineeringMedicine (General)R5-920BiochemistryQD415-436ENStem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Stem cells Neurodegeneration Biofunctionalisation Cell scaffold Implantation Tissue engineering Medicine (General) R5-920 Biochemistry QD415-436
spellingShingle	Stem cells Neurodegeneration Biofunctionalisation Cell scaffold Implantation Tissue engineering Medicine (General) R5-920 Biochemistry QD415-436 Miranda Robbins Venkat Pisupati Roberta Azzarelli Samer I. Nehme Roger A. Barker Ljiljana Fruk Gabriele S. Kaminski Schierle Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
description	Abstract Background Stem cell-based therapies for neurodegenerative diseases like Parkinson’s disease are a promising approach in regenerative medicine and are now moving towards early stage clinical trials. However, a number of challenges remain including the ability to grow stem cells in vitro on a 3-dimensional scaffold, as well as their loss, by leakage or cell death, post-implantation. These issues could, however, be helped through the use of scaffolds that support the growth and differentiation of stem cells both in vitro and in vivo. The present study focuses on the use of bacterial cellulose as an in vitro scaffold to promote the growth of different stem cell-derived cell types. Bacterial cellulose was used because of its remarkable properties such as its wettability, ability to retain water and low stiffness, all of which is similar to that found in brain tissue. Methods We cultured human embryonic stem cell-derived progenitor cells on bacterial cellulose with growth factors that were covalently functionalised to the surface via silanisation. Epifluorescence microscopy and immunofluorescence were used to detect the differentiation of stem cells into dopaminergic ventral midbrain progenitor cells. We then quantified the proportion of cells that differentiated into progenitor cells and compared the effect of growing cells on biofunctionalised cellulose versus standard cellulose. Results We show that the covalent functionalisation of bacterial cellulose sheets with bioactive peptides improves the growth and differentiation of human pluripotent stem cells into dopaminergic neuronal progenitors. Conclusions This study suggests that the biocompatible material, bacterial cellulose, has potential applications in cell therapy approaches as a means to repair damage to the central nervous system, such as in Parkinson’s disease but also in tissue engineering.
format	article
author	Miranda Robbins Venkat Pisupati Roberta Azzarelli Samer I. Nehme Roger A. Barker Ljiljana Fruk Gabriele S. Kaminski Schierle
author_facet	Miranda Robbins Venkat Pisupati Roberta Azzarelli Samer I. Nehme Roger A. Barker Ljiljana Fruk Gabriele S. Kaminski Schierle
author_sort	Miranda Robbins
title	Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
title_short	Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
title_full	Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
title_fullStr	Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
title_full_unstemmed	Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
title_sort	biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
publisher	BMC
publishDate	2021
url	https://doaj.org/article/64d85d1a1edc4887b5086fb9bb822a05
work_keys_str_mv	AT mirandarobbins biofunctionalisedbacterialcellulosescaffoldsupportsthepatterningandexpansionofhumanembryonicstemcellderiveddopaminergicprogenitorcells AT venkatpisupati biofunctionalisedbacterialcellulosescaffoldsupportsthepatterningandexpansionofhumanembryonicstemcellderiveddopaminergicprogenitorcells AT robertaazzarelli biofunctionalisedbacterialcellulosescaffoldsupportsthepatterningandexpansionofhumanembryonicstemcellderiveddopaminergicprogenitorcells AT samerinehme biofunctionalisedbacterialcellulosescaffoldsupportsthepatterningandexpansionofhumanembryonicstemcellderiveddopaminergicprogenitorcells AT rogerabarker biofunctionalisedbacterialcellulosescaffoldsupportsthepatterningandexpansionofhumanembryonicstemcellderiveddopaminergicprogenitorcells AT ljiljanafruk biofunctionalisedbacterialcellulosescaffoldsupportsthepatterningandexpansionofhumanembryonicstemcellderiveddopaminergicprogenitorcells AT gabrieleskaminskischierle biofunctionalisedbacterialcellulosescaffoldsupportsthepatterningandexpansionofhumanembryonicstemcellderiveddopaminergicprogenitorcells
_version_	1718429407796789248

Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells

Ejemplares similares