MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure

Abstract Although several risk factors such as infarct size have been identified, the progression of heart failure (HF) remains difficult to predict in clinical practice. Using an experimental rat model of post-myocardial infarction (MI), we previously identified 45 proteins differentially modulated...

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Autores principales:	Emilie Dubois-Deruy, Marie Cuvelliez, Jan Fiedler, Henri Charrier, Paul Mulder, Eleonore Hebbar, Angelika Pfanne, Olivia Beseme, Maggy Chwastyniak, Philippe Amouyel, Vincent Richard, Christophe Bauters, Thomas Thum, Florence Pinet
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	miRNAs SOD1 Protein Expression Target Sodium Human Cardiomyocytes High Remodeling Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64da9270c0a8427f988f904caedd3058
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spelling	oai:doaj.org-article:64da9270c0a8427f988f904caedd30582021-12-02T11:50:55ZMicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure10.1038/s41598-017-15011-62045-2322https://doaj.org/article/64da9270c0a8427f988f904caedd30582017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15011-6https://doaj.org/toc/2045-2322Abstract Although several risk factors such as infarct size have been identified, the progression of heart failure (HF) remains difficult to predict in clinical practice. Using an experimental rat model of post-myocardial infarction (MI), we previously identified 45 proteins differentially modulated during HF by proteomic analysis. This study sought to identify microRNAs (miRNAs) able to regulate these proteins and to test their relevance as biomarkers for HF. In silico bioinformatical analysis selected 13 miRNAs related to the 45 proteins previously identified. These miRNAs were analyzed in the rat and in cohorts of patients phenotyped for left ventricular remodeling (LVR). We identified that 3 miRNAs, miR-21-5p, miR-23a-3p and miR-222-3p, and their target Mn superoxide dismutase (SOD2) were significantly increased in LV and plasma of HF-rats. We found by luciferase activity a direct interaction of miR-222-3p with 3′UTR of SOD2. Transfection of human cardiomyocytes with miR-222-3p mimic or inhibitor induced respectively a decrease and an increase of SOD2 expression. Circulating levels of the 3 miRNAs and their target SOD2 were associated with high LVR post-MI in REVE-2 patients. We demonstrated for the first time the potential of microRNAs regulating SOD2 as new circulating biomarkers of HF.Emilie Dubois-DeruyMarie CuvelliezJan FiedlerHenri CharrierPaul MulderEleonore HebbarAngelika PfanneOlivia BesemeMaggy ChwastyniakPhilippe AmouyelVincent RichardChristophe BautersThomas ThumFlorence PinetNature PortfolioarticlemiRNAsSOD1 Protein ExpressionTarget SodiumHuman CardiomyocytesHigh RemodelingMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	miRNAs SOD1 Protein Expression Target Sodium Human Cardiomyocytes High Remodeling Medicine R Science Q
spellingShingle	miRNAs SOD1 Protein Expression Target Sodium Human Cardiomyocytes High Remodeling Medicine R Science Q Emilie Dubois-Deruy Marie Cuvelliez Jan Fiedler Henri Charrier Paul Mulder Eleonore Hebbar Angelika Pfanne Olivia Beseme Maggy Chwastyniak Philippe Amouyel Vincent Richard Christophe Bauters Thomas Thum Florence Pinet MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure
description	Abstract Although several risk factors such as infarct size have been identified, the progression of heart failure (HF) remains difficult to predict in clinical practice. Using an experimental rat model of post-myocardial infarction (MI), we previously identified 45 proteins differentially modulated during HF by proteomic analysis. This study sought to identify microRNAs (miRNAs) able to regulate these proteins and to test their relevance as biomarkers for HF. In silico bioinformatical analysis selected 13 miRNAs related to the 45 proteins previously identified. These miRNAs were analyzed in the rat and in cohorts of patients phenotyped for left ventricular remodeling (LVR). We identified that 3 miRNAs, miR-21-5p, miR-23a-3p and miR-222-3p, and their target Mn superoxide dismutase (SOD2) were significantly increased in LV and plasma of HF-rats. We found by luciferase activity a direct interaction of miR-222-3p with 3′UTR of SOD2. Transfection of human cardiomyocytes with miR-222-3p mimic or inhibitor induced respectively a decrease and an increase of SOD2 expression. Circulating levels of the 3 miRNAs and their target SOD2 were associated with high LVR post-MI in REVE-2 patients. We demonstrated for the first time the potential of microRNAs regulating SOD2 as new circulating biomarkers of HF.
format	article
author	Emilie Dubois-Deruy Marie Cuvelliez Jan Fiedler Henri Charrier Paul Mulder Eleonore Hebbar Angelika Pfanne Olivia Beseme Maggy Chwastyniak Philippe Amouyel Vincent Richard Christophe Bauters Thomas Thum Florence Pinet
author_facet	Emilie Dubois-Deruy Marie Cuvelliez Jan Fiedler Henri Charrier Paul Mulder Eleonore Hebbar Angelika Pfanne Olivia Beseme Maggy Chwastyniak Philippe Amouyel Vincent Richard Christophe Bauters Thomas Thum Florence Pinet
author_sort	Emilie Dubois-Deruy
title	MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure
title_short	MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure
title_full	MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure
title_fullStr	MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure
title_full_unstemmed	MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure
title_sort	micrornas regulating superoxide dismutase 2 are new circulating biomarkers of heart failure
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/64da9270c0a8427f988f904caedd3058
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MicroRNAs regulating superoxide dismutase 2 are new circulating biomarkers of heart failure

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