Identification and characterization of profilin gene family in rice

Background: Profilin proteins (PRFs) are small (12–15 kD) actin-binding protein, which play a significant role in cytoskeleton dynamics and plant development via regulating actin polymerization. Profilins have been well documented in Arabidopsis, Zea mays L. as well as Phaseolus vulgaris, however no...

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Autores principales:	Yanli Zhang, Guojun Dong, Limin Wu, Fei Chen, Yanchun Yu, Dianrong Ma
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Abiotic stress Actin polymerization Cytoskeleton Genome-wide analysis Hormone Oryza sativa L. Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/64dbad3191f6449d9b51ea828b8133dd
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spelling	oai:doaj.org-article:64dbad3191f6449d9b51ea828b8133dd2021-11-06T04:20:27ZIdentification and characterization of profilin gene family in rice0717-345810.1016/j.ejbt.2021.08.004https://doaj.org/article/64dbad3191f6449d9b51ea828b8133dd2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0717345821000439https://doaj.org/toc/0717-3458Background: Profilin proteins (PRFs) are small (12–15 kD) actin-binding protein, which play a significant role in cytoskeleton dynamics and plant development via regulating actin polymerization. Profilins have been well documented in Arabidopsis, Zea mays L. as well as Phaseolus vulgaris, however no such fully characterization of rice (Oryza sativa L.) profilin gene family has been reported thus far. Result: In the present study, a comprehensive genome-wide analysis of rice PRF genes was completed and three members were identified. OsPRF1 and OsPRF2 shared 98.5% similarity (6 nucleotide divergence), but the deduced amino acid sequences of OsPRF1 and OsPRF2 are fully identical. In contrast, the OsPRF3 presents relatively lower similarity with OsPRF1 and OsPRF2. Phylogenetic analysis also support that OsPRF1 has a closer relationship with OsPRF2. Expression pattern analysis revealed the differential expression of OsPRFs in tissues of mature plant, which suggested the potential spatial functional specificity for rice profilin genes. Subcellular localization analysis revealed the OsPRFs were localized in cytoplasm and nucleus and all of them could bind actin monomers. Furthermore, abiotic stresses and hormones treatments assay indicated that the three OsPRF genes could be differentially regulated, suggesting that OsPRF genes might participate in different stress processes in rice. Conclusions: Taken together, our study provides a comprehensive analysis of the OsPRF gene family and will provide a basis for further studies on their roles in rice development and in response to abiotic stresses.How to cite: Zhang Y, Dong G, Wu L, et al. Identification and characterization of profilin gene family in rice. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.08.004.Yanli ZhangGuojun DongLimin WuFei ChenYanchun YuDianrong MaElsevierarticleAbiotic stressActin polymerizationCytoskeletonGenome-wide analysisHormoneOryza sativa L.BiotechnologyTP248.13-248.65Biology (General)QH301-705.5ENElectronic Journal of Biotechnology, Vol 54, Iss , Pp 47-59 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Abiotic stress Actin polymerization Cytoskeleton Genome-wide analysis Hormone Oryza sativa L. Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
spellingShingle	Abiotic stress Actin polymerization Cytoskeleton Genome-wide analysis Hormone Oryza sativa L. Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Yanli Zhang Guojun Dong Limin Wu Fei Chen Yanchun Yu Dianrong Ma Identification and characterization of profilin gene family in rice
description	Background: Profilin proteins (PRFs) are small (12–15 kD) actin-binding protein, which play a significant role in cytoskeleton dynamics and plant development via regulating actin polymerization. Profilins have been well documented in Arabidopsis, Zea mays L. as well as Phaseolus vulgaris, however no such fully characterization of rice (Oryza sativa L.) profilin gene family has been reported thus far. Result: In the present study, a comprehensive genome-wide analysis of rice PRF genes was completed and three members were identified. OsPRF1 and OsPRF2 shared 98.5% similarity (6 nucleotide divergence), but the deduced amino acid sequences of OsPRF1 and OsPRF2 are fully identical. In contrast, the OsPRF3 presents relatively lower similarity with OsPRF1 and OsPRF2. Phylogenetic analysis also support that OsPRF1 has a closer relationship with OsPRF2. Expression pattern analysis revealed the differential expression of OsPRFs in tissues of mature plant, which suggested the potential spatial functional specificity for rice profilin genes. Subcellular localization analysis revealed the OsPRFs were localized in cytoplasm and nucleus and all of them could bind actin monomers. Furthermore, abiotic stresses and hormones treatments assay indicated that the three OsPRF genes could be differentially regulated, suggesting that OsPRF genes might participate in different stress processes in rice. Conclusions: Taken together, our study provides a comprehensive analysis of the OsPRF gene family and will provide a basis for further studies on their roles in rice development and in response to abiotic stresses.How to cite: Zhang Y, Dong G, Wu L, et al. Identification and characterization of profilin gene family in rice. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.08.004.
format	article
author	Yanli Zhang Guojun Dong Limin Wu Fei Chen Yanchun Yu Dianrong Ma
author_facet	Yanli Zhang Guojun Dong Limin Wu Fei Chen Yanchun Yu Dianrong Ma
author_sort	Yanli Zhang
title	Identification and characterization of profilin gene family in rice
title_short	Identification and characterization of profilin gene family in rice
title_full	Identification and characterization of profilin gene family in rice
title_fullStr	Identification and characterization of profilin gene family in rice
title_full_unstemmed	Identification and characterization of profilin gene family in rice
title_sort	identification and characterization of profilin gene family in rice
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/64dbad3191f6449d9b51ea828b8133dd
work_keys_str_mv	AT yanlizhang identificationandcharacterizationofprofilingenefamilyinrice AT guojundong identificationandcharacterizationofprofilingenefamilyinrice AT liminwu identificationandcharacterizationofprofilingenefamilyinrice AT feichen identificationandcharacterizationofprofilingenefamilyinrice AT yanchunyu identificationandcharacterizationofprofilingenefamilyinrice AT dianrongma identificationandcharacterizationofprofilingenefamilyinrice
_version_	1718443920476602368

Identification and characterization of profilin gene family in rice

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