Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway

Abstract Background Diabetic kidney disease (DKD) is a major contributor to end-stage renal disease. Several microRNAs (miRNAs) have been found to be enriched in exosomes of DKD patients, but it remains unclear if any of these miRNAs play an important role in the pathogenesis of DKD. Methods Exosome...

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Autores principales: Chan Gao, Benyong Wang, Qi Chen, Ming Wang, Xiao Fei, Ning Zhao
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Publicado: Nature Publishing Group 2021
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Acceso en línea:https://doaj.org/article/64dc68e946f04f71958e411a2e740f0d
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spelling oai:doaj.org-article:64dc68e946f04f71958e411a2e740f0d2021-11-08T10:56:50ZSerum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway10.1038/s41387-021-00175-y2044-4052https://doaj.org/article/64dc68e946f04f71958e411a2e740f0d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41387-021-00175-yhttps://doaj.org/toc/2044-4052Abstract Background Diabetic kidney disease (DKD) is a major contributor to end-stage renal disease. Several microRNAs (miRNAs) have been found to be enriched in exosomes of DKD patients, but it remains unclear if any of these miRNAs play an important role in the pathogenesis of DKD. Methods Exosomes from diabetic kidney disease (DKD) patients were isolated, and the expression of miR-4449 was measured by qRT-PCR. Reactive oxygen species (ROS) was determined by DCDFA assay kit, and pyroptosis was measured by quantifying the level of activated caspase 1. mRNA and protein levels were quantified by qRT-PCR and WB. Results In this study, we demonstrated that miR-4449 is enriched in the serum exosomes of DKD patients, and these exosomes regulate the expression of pro-inflammatory cytokines, ROS levels, and pyroptosis through miR-4449. Conclusions Our study uncovered a novel mechanism for the progression of DKD that is mediated through miR-4449 in serum exosomes, which highlights an important role for exosomes in the pathogenesis of DKD.Chan GaoBenyong WangQi ChenMing WangXiao FeiNing ZhaoNature Publishing GrouparticleNutritional diseases. Deficiency diseasesRC620-627ENNutrition & Diabetes, Vol 11, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Nutritional diseases. Deficiency diseases
RC620-627
spellingShingle Nutritional diseases. Deficiency diseases
RC620-627
Chan Gao
Benyong Wang
Qi Chen
Ming Wang
Xiao Fei
Ning Zhao
Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway
description Abstract Background Diabetic kidney disease (DKD) is a major contributor to end-stage renal disease. Several microRNAs (miRNAs) have been found to be enriched in exosomes of DKD patients, but it remains unclear if any of these miRNAs play an important role in the pathogenesis of DKD. Methods Exosomes from diabetic kidney disease (DKD) patients were isolated, and the expression of miR-4449 was measured by qRT-PCR. Reactive oxygen species (ROS) was determined by DCDFA assay kit, and pyroptosis was measured by quantifying the level of activated caspase 1. mRNA and protein levels were quantified by qRT-PCR and WB. Results In this study, we demonstrated that miR-4449 is enriched in the serum exosomes of DKD patients, and these exosomes regulate the expression of pro-inflammatory cytokines, ROS levels, and pyroptosis through miR-4449. Conclusions Our study uncovered a novel mechanism for the progression of DKD that is mediated through miR-4449 in serum exosomes, which highlights an important role for exosomes in the pathogenesis of DKD.
format article
author Chan Gao
Benyong Wang
Qi Chen
Ming Wang
Xiao Fei
Ning Zhao
author_facet Chan Gao
Benyong Wang
Qi Chen
Ming Wang
Xiao Fei
Ning Zhao
author_sort Chan Gao
title Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway
title_short Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway
title_full Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway
title_fullStr Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway
title_full_unstemmed Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway
title_sort serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the mir-4449/hic1 pathway
publisher Nature Publishing Group
publishDate 2021
url https://doaj.org/article/64dc68e946f04f71958e411a2e740f0d
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