Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.

<h4>Background</h4>Traditional Chinese medicinal herbs Cortex Moutan and Radix Salviae Milthiorrhizaeare are prescribed together for their putative cardioprotective effects in clinical practice. However, the rationale of the combined use remains unclear. The present study was designed to...

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Autores principales:	Hua Li, Yan-Hua Xie, Qian Yang, Si-Wang Wang, Bang-Le Zhang, Jian-Bo Wang, Wei Cao, Lin-Lin Bi, Ji-Yuan Sun, Shan Miao, Jing Hu, Xuan-Xuan Zhou, Peng-Cheng Qiu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64dc766e8a654a8eb88353ad538196ed
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spelling	oai:doaj.org-article:64dc766e8a654a8eb88353ad538196ed2021-11-18T08:09:53ZCardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.1932-620310.1371/journal.pone.0048872https://doaj.org/article/64dc766e8a654a8eb88353ad538196ed2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23139821/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Traditional Chinese medicinal herbs Cortex Moutan and Radix Salviae Milthiorrhizaeare are prescribed together for their putative cardioprotective effects in clinical practice. However, the rationale of the combined use remains unclear. The present study was designed to investigate the cardioprotective effects of paeonol and danshensu (representative active ingredient of Cortex Moutan and Radix Salviae Milthiorrhizae, respectively) on isoproterenol-induced myocardial infarction in rats and its underlying mechanisms.<h4>Methodology</h4>Paeonol (80 mg kg(-1)) and danshensu (160 mg kg(-1)) were administered orally to Sprague Dawley rats in individual or in combination for 21 days. At the end of this period, rats were administered isoproterenol (85 mg kg(-1)) subcutaneously to induce myocardial injury. After induction, rats were anaesthetized with pentobarbital sodium (35 mg kg(-1)) to record electrocardiogram, then sacrificed and biochemical assays of the heart tissues were performed.<h4>Principal findings</h4>Induction of rats with isoproterenol resulted in a marked (P<0.001) elevation in ST-segment, infarct size, level of serum marker enzymes (CK-MB, LDH, AST and ALT), cTnI, TBARS, protein expression of Bax and Caspase-3 and a significant decrease in the activities of endogenous antioxidants (SOD, CAT, GPx, GR, and GST) and protein expression of Bcl-2. Pretreatment with paeonol and danshensu combination showed a significant (P<0.001) decrease in ST-segment elevation, infarct size, cTnI, TBARS, protein expression of Bax and Caspase-3 and a significant increase in the activities of endogenous antioxidants and protein expression of Bcl-2 and Nrf2 when compared with individual treated groups.<h4>Conclusions/significance</h4>This study demonstrates the cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial infarction in rats. The mechanism might be associated with the enhancement of antioxidant defense system through activating of Nrf2 signaling and anti-apoptosis through regulating Bax, Bcl-2 and Caspase-3. It could provide experimental evidence to support the rationality of combinatorial use of traditional Chinese medicine in clinical practice.Hua LiYan-Hua XieQian YangSi-Wang WangBang-Le ZhangJian-Bo WangWei CaoLin-Lin BiJi-Yuan SunShan MiaoJing HuXuan-Xuan ZhouPeng-Cheng QiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48872 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hua Li Yan-Hua Xie Qian Yang Si-Wang Wang Bang-Le Zhang Jian-Bo Wang Wei Cao Lin-Lin Bi Ji-Yuan Sun Shan Miao Jing Hu Xuan-Xuan Zhou Peng-Cheng Qiu Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.
description	<h4>Background</h4>Traditional Chinese medicinal herbs Cortex Moutan and Radix Salviae Milthiorrhizaeare are prescribed together for their putative cardioprotective effects in clinical practice. However, the rationale of the combined use remains unclear. The present study was designed to investigate the cardioprotective effects of paeonol and danshensu (representative active ingredient of Cortex Moutan and Radix Salviae Milthiorrhizae, respectively) on isoproterenol-induced myocardial infarction in rats and its underlying mechanisms.<h4>Methodology</h4>Paeonol (80 mg kg(-1)) and danshensu (160 mg kg(-1)) were administered orally to Sprague Dawley rats in individual or in combination for 21 days. At the end of this period, rats were administered isoproterenol (85 mg kg(-1)) subcutaneously to induce myocardial injury. After induction, rats were anaesthetized with pentobarbital sodium (35 mg kg(-1)) to record electrocardiogram, then sacrificed and biochemical assays of the heart tissues were performed.<h4>Principal findings</h4>Induction of rats with isoproterenol resulted in a marked (P<0.001) elevation in ST-segment, infarct size, level of serum marker enzymes (CK-MB, LDH, AST and ALT), cTnI, TBARS, protein expression of Bax and Caspase-3 and a significant decrease in the activities of endogenous antioxidants (SOD, CAT, GPx, GR, and GST) and protein expression of Bcl-2. Pretreatment with paeonol and danshensu combination showed a significant (P<0.001) decrease in ST-segment elevation, infarct size, cTnI, TBARS, protein expression of Bax and Caspase-3 and a significant increase in the activities of endogenous antioxidants and protein expression of Bcl-2 and Nrf2 when compared with individual treated groups.<h4>Conclusions/significance</h4>This study demonstrates the cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial infarction in rats. The mechanism might be associated with the enhancement of antioxidant defense system through activating of Nrf2 signaling and anti-apoptosis through regulating Bax, Bcl-2 and Caspase-3. It could provide experimental evidence to support the rationality of combinatorial use of traditional Chinese medicine in clinical practice.
format	article
author	Hua Li Yan-Hua Xie Qian Yang Si-Wang Wang Bang-Le Zhang Jian-Bo Wang Wei Cao Lin-Lin Bi Ji-Yuan Sun Shan Miao Jing Hu Xuan-Xuan Zhou Peng-Cheng Qiu
author_facet	Hua Li Yan-Hua Xie Qian Yang Si-Wang Wang Bang-Le Zhang Jian-Bo Wang Wei Cao Lin-Lin Bi Ji-Yuan Sun Shan Miao Jing Hu Xuan-Xuan Zhou Peng-Cheng Qiu
author_sort	Hua Li
title	Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.
title_short	Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.
title_full	Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.
title_fullStr	Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.
title_full_unstemmed	Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.
title_sort	cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/64dc766e8a654a8eb88353ad538196ed
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Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats.

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