Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity

With regard to the emerging health threat by antibiotic resistant bacteria, bacteriocins are considered as a promising way to overcome this urgent problem. In this work, a hitherto unknown bacteriocin from the terrestrial cyanobacterium Chroococcidiopsis cubana was heterologously expressed in Escher...

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Autores principales:	Marco Witthohn, Dorina Strieth, Simone Eggert, Stefan Kins, Roland Ulber, Kai Muffler
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cyanobacteria Bacteriocins Chroococcidiopsis cubana Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/64e096edfb2b40d58892cb29fb87f2eb
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spelling	oai:doaj.org-article:64e096edfb2b40d58892cb29fb87f2eb2021-11-18T04:51:31ZHeterologous production of a cyanobacterial bacteriocin with potent antibacterial activity2590-262810.1016/j.crbiot.2021.10.002https://doaj.org/article/64e096edfb2b40d58892cb29fb87f2eb2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590262821000307https://doaj.org/toc/2590-2628With regard to the emerging health threat by antibiotic resistant bacteria, bacteriocins are considered as a promising way to overcome this urgent problem. In this work, a hitherto unknown bacteriocin from the terrestrial cyanobacterium Chroococcidiopsis cubana was heterologously expressed in Escherichia coli, purified to homogeneity and tested for activity against several bacteria and one yeast species. The compound showed potent bacteriolytic activity against several Gram-positive bacteria and slight activity against one Gram-negative strain. The bacteriocin had no cytotoxic impact on mouse neuroblastoma N2a cells, indicating its potential for treatment against Gram-positive bacterial pathogens in human diseases.Marco WitthohnDorina StriethSimone EggertStefan KinsRoland UlberKai MufflerElsevierarticleCyanobacteriaBacteriocinsChroococcidiopsis cubanaBiotechnologyTP248.13-248.65ENCurrent Research in Biotechnology, Vol 3, Iss , Pp 281-287 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cyanobacteria Bacteriocins Chroococcidiopsis cubana Biotechnology TP248.13-248.65
spellingShingle	Cyanobacteria Bacteriocins Chroococcidiopsis cubana Biotechnology TP248.13-248.65 Marco Witthohn Dorina Strieth Simone Eggert Stefan Kins Roland Ulber Kai Muffler Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity
description	With regard to the emerging health threat by antibiotic resistant bacteria, bacteriocins are considered as a promising way to overcome this urgent problem. In this work, a hitherto unknown bacteriocin from the terrestrial cyanobacterium Chroococcidiopsis cubana was heterologously expressed in Escherichia coli, purified to homogeneity and tested for activity against several bacteria and one yeast species. The compound showed potent bacteriolytic activity against several Gram-positive bacteria and slight activity against one Gram-negative strain. The bacteriocin had no cytotoxic impact on mouse neuroblastoma N2a cells, indicating its potential for treatment against Gram-positive bacterial pathogens in human diseases.
format	article
author	Marco Witthohn Dorina Strieth Simone Eggert Stefan Kins Roland Ulber Kai Muffler
author_facet	Marco Witthohn Dorina Strieth Simone Eggert Stefan Kins Roland Ulber Kai Muffler
author_sort	Marco Witthohn
title	Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity
title_short	Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity
title_full	Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity
title_fullStr	Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity
title_full_unstemmed	Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity
title_sort	heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/64e096edfb2b40d58892cb29fb87f2eb
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Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity

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