FEATURES OF LUMINOL- AND LUCIGENIN-INDUCED CHEMILUMINESCENCE OF NEUTROPHILIC GRANULOCYTES IN PATIENTS WITH CHRONIC RHINOSINUSITIS
Studies of functional activity of neutrophilic granulocytes from patients with chronic rhinosinusitis was performed using luminol- or lucigenin-dependent chemiluminescence. We have revealed increased production of reactive oxygen species (ROS) both under basal conditions, and in zymozan-induced chem...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | RU |
| Publicado: |
SPb RAACI
2014
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/64e3515f0c69404191ba5901067a0e64 |
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| Sumario: | Studies of functional activity of neutrophilic granulocytes from patients with chronic rhinosinusitis was performed using luminol- or lucigenin-dependent chemiluminescence. We have revealed increased production of reactive oxygen species (ROS) both under basal conditions, and in zymozan-induced chemiluminescent reaction, employing luminol as an activator, and determining total functional activity of neutrophils. Meanwhile, in lucigenin-induced chemiluminescence associated with production of superoxide anion radicals, a delayed ROS production was revealed, both in spontaneous and zymozan-induced response. One may suggest that, when studying these pathological events in neutrophils, ROS production in the cells proceeds mostly by myeloperoxidase pathway. |
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