Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages
Masaharu Murata,1,2 Sayoko Narahara,1,2 Kaori Umezaki,1 Riki Toita,1,2 Shigekazu Tabata,1 Jing Shu Piao,1 Kana Abe,1 Jeong-Hun Kang,3 Kenoki Ohuchida,1,4 Lin Cui,4 Makoto Hashizume1,21Department of Advanced Medical Initiatives, Faculty of Medical Science, Kyushu University, Fukuoka, Japan; 2Innovati...
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Dove Medical Press
2012
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oai:doaj.org-article:64ebcf5597634feb8c9c5b73cc8312862021-12-02T07:20:24ZLiver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages1176-91141178-2013https://doaj.org/article/64ebcf5597634feb8c9c5b73cc8312862012-08-01T00:00:00Zhttp://www.dovepress.com/liver-cell-specific-targeting-by-the-pres1-domain-of-hepatitis-b-virus-a10652https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Masaharu Murata,1,2 Sayoko Narahara,1,2 Kaori Umezaki,1 Riki Toita,1,2 Shigekazu Tabata,1 Jing Shu Piao,1 Kana Abe,1 Jeong-Hun Kang,3 Kenoki Ohuchida,1,4 Lin Cui,4 Makoto Hashizume1,21Department of Advanced Medical Initiatives, Faculty of Medical Science, Kyushu University, Fukuoka, Japan; 2Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka, Japan; 3Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan; 4Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanAbstract: Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety of molecules, including therapeutic or diagnostic agents, can be encapsulated. These properties yield these particles suitable for a new class of drug delivery carrier, or as a bioimaging reagent that might respond to biochemical signals in many different cellular processes. We report here the design, synthesis, and biological characterization of a hepatocyte-specific nanocage carrying small heat-shock protein. These nanoscale protein cages, with a targeting peptide composed of a preS1 derivative from the hepatitis B virus on their surfaces, were prepared by genetic engineering techniques. PreS1-carrying nanocages showed lower cytotoxicity and significantly higher specificity for human hepatocyte cell lines than other cell lines in vitro. These results suggested that small heat-shock protein-based nanocages present great potential for the development of effective targeted delivery of various agents to specific cells.Keywords: protein nanocages, drug delivery system, hepatocyte cell lines specific, hepatitis B virusMurata MNarahara SUmezaki KToita RTabata SPiao JSAbe KKang JHOhuchida KCui LHashizume MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4353-4362 (2012) |
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Medicine (General) R5-920 |
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Medicine (General) R5-920 Murata M Narahara S Umezaki K Toita R Tabata S Piao JS Abe K Kang JH Ohuchida K Cui L Hashizume M Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages |
| description |
Masaharu Murata,1,2 Sayoko Narahara,1,2 Kaori Umezaki,1 Riki Toita,1,2 Shigekazu Tabata,1 Jing Shu Piao,1 Kana Abe,1 Jeong-Hun Kang,3 Kenoki Ohuchida,1,4 Lin Cui,4 Makoto Hashizume1,21Department of Advanced Medical Initiatives, Faculty of Medical Science, Kyushu University, Fukuoka, Japan; 2Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka, Japan; 3Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan; 4Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanAbstract: Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety of molecules, including therapeutic or diagnostic agents, can be encapsulated. These properties yield these particles suitable for a new class of drug delivery carrier, or as a bioimaging reagent that might respond to biochemical signals in many different cellular processes. We report here the design, synthesis, and biological characterization of a hepatocyte-specific nanocage carrying small heat-shock protein. These nanoscale protein cages, with a targeting peptide composed of a preS1 derivative from the hepatitis B virus on their surfaces, were prepared by genetic engineering techniques. PreS1-carrying nanocages showed lower cytotoxicity and significantly higher specificity for human hepatocyte cell lines than other cell lines in vitro. These results suggested that small heat-shock protein-based nanocages present great potential for the development of effective targeted delivery of various agents to specific cells.Keywords: protein nanocages, drug delivery system, hepatocyte cell lines specific, hepatitis B virus |
| format |
article |
| author |
Murata M Narahara S Umezaki K Toita R Tabata S Piao JS Abe K Kang JH Ohuchida K Cui L Hashizume M |
| author_facet |
Murata M Narahara S Umezaki K Toita R Tabata S Piao JS Abe K Kang JH Ohuchida K Cui L Hashizume M |
| author_sort |
Murata M |
| title |
Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages |
| title_short |
Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages |
| title_full |
Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages |
| title_fullStr |
Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages |
| title_full_unstemmed |
Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages |
| title_sort |
liver cell specific targeting by the pres1 domain of hepatitis b virus surface antigen displayed on protein nanocages |
| publisher |
Dove Medical Press |
| publishDate |
2012 |
| url |
https://doaj.org/article/64ebcf5597634feb8c9c5b73cc831286 |
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