Label-free cell cycle analysis for high-throughput imaging flow cytometry

Imaging flow cytometry enables high-throughput acquisition of fluorescence, brightfield and darkfield images of biological cells. Here, Blasi et al.demonstrate that applying machine learning algorithms on brightfield and darkfield images can detect cellular phenotypes without the need for fluorescen...

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Autores principales:	Thomas Blasi, Holger Hennig, Huw D. Summers, Fabian J. Theis, Joana Cerveira, James O. Patterson, Derek Davies, Andrew Filby, Anne E. Carpenter, Paul Rees
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/64eccd9285fd45858c93a445b1345a3c
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spelling	oai:doaj.org-article:64eccd9285fd45858c93a445b1345a3c2021-12-02T15:33:49ZLabel-free cell cycle analysis for high-throughput imaging flow cytometry10.1038/ncomms102562041-1723https://doaj.org/article/64eccd9285fd45858c93a445b1345a3c2016-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms10256https://doaj.org/toc/2041-1723Imaging flow cytometry enables high-throughput acquisition of fluorescence, brightfield and darkfield images of biological cells. Here, Blasi et al.demonstrate that applying machine learning algorithms on brightfield and darkfield images can detect cellular phenotypes without the need for fluorescent stains, enabling label-free assays.Thomas BlasiHolger HennigHuw D. SummersFabian J. TheisJoana CerveiraJames O. PattersonDerek DaviesAndrew FilbyAnne E. CarpenterPaul ReesNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-9 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Thomas Blasi Holger Hennig Huw D. Summers Fabian J. Theis Joana Cerveira James O. Patterson Derek Davies Andrew Filby Anne E. Carpenter Paul Rees Label-free cell cycle analysis for high-throughput imaging flow cytometry
description	Imaging flow cytometry enables high-throughput acquisition of fluorescence, brightfield and darkfield images of biological cells. Here, Blasi et al.demonstrate that applying machine learning algorithms on brightfield and darkfield images can detect cellular phenotypes without the need for fluorescent stains, enabling label-free assays.
format	article
author	Thomas Blasi Holger Hennig Huw D. Summers Fabian J. Theis Joana Cerveira James O. Patterson Derek Davies Andrew Filby Anne E. Carpenter Paul Rees
author_facet	Thomas Blasi Holger Hennig Huw D. Summers Fabian J. Theis Joana Cerveira James O. Patterson Derek Davies Andrew Filby Anne E. Carpenter Paul Rees
author_sort	Thomas Blasi
title	Label-free cell cycle analysis for high-throughput imaging flow cytometry
title_short	Label-free cell cycle analysis for high-throughput imaging flow cytometry
title_full	Label-free cell cycle analysis for high-throughput imaging flow cytometry
title_fullStr	Label-free cell cycle analysis for high-throughput imaging flow cytometry
title_full_unstemmed	Label-free cell cycle analysis for high-throughput imaging flow cytometry
title_sort	label-free cell cycle analysis for high-throughput imaging flow cytometry
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/64eccd9285fd45858c93a445b1345a3c
work_keys_str_mv	AT thomasblasi labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT holgerhennig labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT huwdsummers labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT fabianjtheis labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT joanacerveira labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT jamesopatterson labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT derekdavies labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT andrewfilby labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT anneecarpenter labelfreecellcycleanalysisforhighthroughputimagingflowcytometry AT paulrees labelfreecellcycleanalysisforhighthroughputimagingflowcytometry
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Label-free cell cycle analysis for high-throughput imaging flow cytometry

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