Three-dimensional morphological analysis of spermatogenesis in aged mouse testes

Abstract Spermatogenesis, which is a continuous process from undifferentiated spermatogonia to spermatozoa in the seminiferous tubules, declines with age. To investigate changes in spermatogenesis with aging, we reconstructed the seminiferous tubules of 12 mice aged 12 to 30 months from serial secti...

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Autores principales:	Taito Nakano, Hiroki Nakata, Suguru Kadomoto, Hiroaki Iwamoto, Hiroshi Yaegashi, Masashi Iijima, Shohei Kawaguchi, Takahiro Nohara, Kazuyoshi Shigehara, Kouji Izumi, Yoshifumi Kadono, Atsushi Mizokami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64f09a8938b4475ca124e3448bb5598f
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spelling	oai:doaj.org-article:64f09a8938b4475ca124e3448bb5598f2021-11-28T12:18:35ZThree-dimensional morphological analysis of spermatogenesis in aged mouse testes10.1038/s41598-021-02443-42045-2322https://doaj.org/article/64f09a8938b4475ca124e3448bb5598f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02443-4https://doaj.org/toc/2045-2322Abstract Spermatogenesis, which is a continuous process from undifferentiated spermatogonia to spermatozoa in the seminiferous tubules, declines with age. To investigate changes in spermatogenesis with aging, we reconstructed the seminiferous tubules of 12 mice aged 12 to 30 months from serial sections and examined age-related and region-specific alterations in the seminiferous epithelium and spermatogenic waves in three dimensions. The basic structure of the seminiferous tubules, including the numbers of tubules, terminating points, branching points, and total tubule length, did not change with age. Age-related alterations in spermatogenesis, primarily assessed by the formation of vacuoles in Sertoli cells, were detected in the seminiferous tubules at 12 months. The proportion of altered tubule segments with impaired spermatogenesis further increased by 24 months, but remained unchanged thereafter. Altered tubule segments were preferentially distributed in tubule areas close to the rete testis and those in the center of the testis. Spermatogenic waves became shorter in length with age. These results provide a basis for examining the decline of spermatogenesis not only with aging, but also in male infertility.Taito NakanoHiroki NakataSuguru KadomotoHiroaki IwamotoHiroshi YaegashiMasashi IijimaShohei KawaguchiTakahiro NoharaKazuyoshi ShigeharaKouji IzumiYoshifumi KadonoAtsushi MizokamiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taito Nakano Hiroki Nakata Suguru Kadomoto Hiroaki Iwamoto Hiroshi Yaegashi Masashi Iijima Shohei Kawaguchi Takahiro Nohara Kazuyoshi Shigehara Kouji Izumi Yoshifumi Kadono Atsushi Mizokami Three-dimensional morphological analysis of spermatogenesis in aged mouse testes
description	Abstract Spermatogenesis, which is a continuous process from undifferentiated spermatogonia to spermatozoa in the seminiferous tubules, declines with age. To investigate changes in spermatogenesis with aging, we reconstructed the seminiferous tubules of 12 mice aged 12 to 30 months from serial sections and examined age-related and region-specific alterations in the seminiferous epithelium and spermatogenic waves in three dimensions. The basic structure of the seminiferous tubules, including the numbers of tubules, terminating points, branching points, and total tubule length, did not change with age. Age-related alterations in spermatogenesis, primarily assessed by the formation of vacuoles in Sertoli cells, were detected in the seminiferous tubules at 12 months. The proportion of altered tubule segments with impaired spermatogenesis further increased by 24 months, but remained unchanged thereafter. Altered tubule segments were preferentially distributed in tubule areas close to the rete testis and those in the center of the testis. Spermatogenic waves became shorter in length with age. These results provide a basis for examining the decline of spermatogenesis not only with aging, but also in male infertility.
format	article
author	Taito Nakano Hiroki Nakata Suguru Kadomoto Hiroaki Iwamoto Hiroshi Yaegashi Masashi Iijima Shohei Kawaguchi Takahiro Nohara Kazuyoshi Shigehara Kouji Izumi Yoshifumi Kadono Atsushi Mizokami
author_facet	Taito Nakano Hiroki Nakata Suguru Kadomoto Hiroaki Iwamoto Hiroshi Yaegashi Masashi Iijima Shohei Kawaguchi Takahiro Nohara Kazuyoshi Shigehara Kouji Izumi Yoshifumi Kadono Atsushi Mizokami
author_sort	Taito Nakano
title	Three-dimensional morphological analysis of spermatogenesis in aged mouse testes
title_short	Three-dimensional morphological analysis of spermatogenesis in aged mouse testes
title_full	Three-dimensional morphological analysis of spermatogenesis in aged mouse testes
title_fullStr	Three-dimensional morphological analysis of spermatogenesis in aged mouse testes
title_full_unstemmed	Three-dimensional morphological analysis of spermatogenesis in aged mouse testes
title_sort	three-dimensional morphological analysis of spermatogenesis in aged mouse testes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/64f09a8938b4475ca124e3448bb5598f
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Three-dimensional morphological analysis of spermatogenesis in aged mouse testes

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