Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.

The regenerative potential declines upon aging. This might be due to cell-intrinsic changes in stem and progenitor cells or to influences by the microenvironment. Mesenchymal stem cells (MSC) raise high hopes in regenerative medicine. They are usually culture expanded in media with fetal calf serum...

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Autores principales:	Michael Lohmann, Gudrun Walenda, Hatim Hemeda, Sylvia Joussen, Wolf Drescher, Stefan Jockenhoevel, Gabriele Hutschenreuter, Martin Zenke, Wolfgang Wagner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64f8faca285e4251aa72465b5446504d
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spelling	oai:doaj.org-article:64f8faca285e4251aa72465b5446504d2021-11-18T07:17:15ZDonor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.1932-620310.1371/journal.pone.0037839https://doaj.org/article/64f8faca285e4251aa72465b5446504d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22662236/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The regenerative potential declines upon aging. This might be due to cell-intrinsic changes in stem and progenitor cells or to influences by the microenvironment. Mesenchymal stem cells (MSC) raise high hopes in regenerative medicine. They are usually culture expanded in media with fetal calf serum (FCS) or other serum supplements such as human platelet lysate (HPL). In this study, we have analyzed the impact of HPL-donor age on culture expansion. 31 single donor derived HPLs (25 to 57 years old) were simultaneously compared for culture of MSC. Proliferation of MSC did not reveal a clear association with platelet counts of HPL donors or growth factors concentrations (PDGF-AB, TGF-β1, bFGF, or IGF-1), but it was significantly higher with HPLs from younger donors (<35 years) as compared to older donors (>45 years). Furthermore, HPLs from older donors increased activity of senescence-associated beta-galactosidase (SA-βgal). HPL-donor age did not affect the fibroblastoid colony-forming unit (CFU-f) frequency, immunophenotype or induction of adipogenic differentiation, whereas osteogenic differentiation was significantly lower with HPLs from older donors. Concentrations of various growth factors (PDGF-AB, TGF-β1, bFGF, IGF-1) or hormones (estradiol, parathormone, leptin, 1,25 vitamin D3) were not associated with HPL-donor age or MSC growth. Taken together, our data support the notion that aging is associated with systemic feedback mechanisms acting on stem and progenitor cells, and this is also relevant for serum supplements in cell culture: HPLs derived from younger donors facilitate enhanced expansion and more pronounced osteogenic differentiation.Michael LohmannGudrun WalendaHatim HemedaSylvia JoussenWolf DrescherStefan JockenhoevelGabriele HutschenreuterMartin ZenkeWolfgang WagnerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37839 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael Lohmann Gudrun Walenda Hatim Hemeda Sylvia Joussen Wolf Drescher Stefan Jockenhoevel Gabriele Hutschenreuter Martin Zenke Wolfgang Wagner Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
description	The regenerative potential declines upon aging. This might be due to cell-intrinsic changes in stem and progenitor cells or to influences by the microenvironment. Mesenchymal stem cells (MSC) raise high hopes in regenerative medicine. They are usually culture expanded in media with fetal calf serum (FCS) or other serum supplements such as human platelet lysate (HPL). In this study, we have analyzed the impact of HPL-donor age on culture expansion. 31 single donor derived HPLs (25 to 57 years old) were simultaneously compared for culture of MSC. Proliferation of MSC did not reveal a clear association with platelet counts of HPL donors or growth factors concentrations (PDGF-AB, TGF-β1, bFGF, or IGF-1), but it was significantly higher with HPLs from younger donors (<35 years) as compared to older donors (>45 years). Furthermore, HPLs from older donors increased activity of senescence-associated beta-galactosidase (SA-βgal). HPL-donor age did not affect the fibroblastoid colony-forming unit (CFU-f) frequency, immunophenotype or induction of adipogenic differentiation, whereas osteogenic differentiation was significantly lower with HPLs from older donors. Concentrations of various growth factors (PDGF-AB, TGF-β1, bFGF, IGF-1) or hormones (estradiol, parathormone, leptin, 1,25 vitamin D3) were not associated with HPL-donor age or MSC growth. Taken together, our data support the notion that aging is associated with systemic feedback mechanisms acting on stem and progenitor cells, and this is also relevant for serum supplements in cell culture: HPLs derived from younger donors facilitate enhanced expansion and more pronounced osteogenic differentiation.
format	article
author	Michael Lohmann Gudrun Walenda Hatim Hemeda Sylvia Joussen Wolf Drescher Stefan Jockenhoevel Gabriele Hutschenreuter Martin Zenke Wolfgang Wagner
author_facet	Michael Lohmann Gudrun Walenda Hatim Hemeda Sylvia Joussen Wolf Drescher Stefan Jockenhoevel Gabriele Hutschenreuter Martin Zenke Wolfgang Wagner
author_sort	Michael Lohmann
title	Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
title_short	Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
title_full	Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
title_fullStr	Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
title_full_unstemmed	Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
title_sort	donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/64f8faca285e4251aa72465b5446504d
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Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.

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