Site-specific gene expression analysis using an automated tissue micro-dissection punching system

Abstract Site-specific gene expression analyses are important for understanding tissue functions. Despite rapid developments in DNA-related technologies, the site-specific analysis of whole genome expression for a tissue remains challenging. Thus, a new tool is required for capturing multiple tissue...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takuya Yoda, Masahito Hosokawa, Kiyofumi Takahashi, Chikako Sakanashi, Haruko Takeyama, Hideki Kambara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64fa6cea83a0462089f492141d799655
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:64fa6cea83a0462089f492141d799655
record_format	dspace
spelling	oai:doaj.org-article:64fa6cea83a0462089f492141d7996552021-12-02T11:40:51ZSite-specific gene expression analysis using an automated tissue micro-dissection punching system10.1038/s41598-017-04616-62045-2322https://doaj.org/article/64fa6cea83a0462089f492141d7996552017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04616-6https://doaj.org/toc/2045-2322Abstract Site-specific gene expression analyses are important for understanding tissue functions. Despite rapid developments in DNA-related technologies, the site-specific analysis of whole genome expression for a tissue remains challenging. Thus, a new tool is required for capturing multiple tissue micro-dissections or single cells while retaining the positional information. Here, we describe the development of such a system, which can pick up micro-dissections by punching a tissue repeatedly in a very short period, e.g., 5 s/sampling cycle. A photo of the punched tissue provides information on the dissected positions, allowing site-specific gene expression analysis. We demonstrate the site-specific analysis of a frozen tissue slice of mouse brain by analyzing many micro-dissections produced from the tissue at a 300-μm pitch. The site-specific analysis provided new insights into the gene expression profiles in a tissue and on tissue functions. The analysis of site-specific whole genome expression may therefore, open new avenues in life science.Takuya YodaMasahito HosokawaKiyofumi TakahashiChikako SakanashiHaruko TakeyamaHideki KambaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takuya Yoda Masahito Hosokawa Kiyofumi Takahashi Chikako Sakanashi Haruko Takeyama Hideki Kambara Site-specific gene expression analysis using an automated tissue micro-dissection punching system
description	Abstract Site-specific gene expression analyses are important for understanding tissue functions. Despite rapid developments in DNA-related technologies, the site-specific analysis of whole genome expression for a tissue remains challenging. Thus, a new tool is required for capturing multiple tissue micro-dissections or single cells while retaining the positional information. Here, we describe the development of such a system, which can pick up micro-dissections by punching a tissue repeatedly in a very short period, e.g., 5 s/sampling cycle. A photo of the punched tissue provides information on the dissected positions, allowing site-specific gene expression analysis. We demonstrate the site-specific analysis of a frozen tissue slice of mouse brain by analyzing many micro-dissections produced from the tissue at a 300-μm pitch. The site-specific analysis provided new insights into the gene expression profiles in a tissue and on tissue functions. The analysis of site-specific whole genome expression may therefore, open new avenues in life science.
format	article
author	Takuya Yoda Masahito Hosokawa Kiyofumi Takahashi Chikako Sakanashi Haruko Takeyama Hideki Kambara
author_facet	Takuya Yoda Masahito Hosokawa Kiyofumi Takahashi Chikako Sakanashi Haruko Takeyama Hideki Kambara
author_sort	Takuya Yoda
title	Site-specific gene expression analysis using an automated tissue micro-dissection punching system
title_short	Site-specific gene expression analysis using an automated tissue micro-dissection punching system
title_full	Site-specific gene expression analysis using an automated tissue micro-dissection punching system
title_fullStr	Site-specific gene expression analysis using an automated tissue micro-dissection punching system
title_full_unstemmed	Site-specific gene expression analysis using an automated tissue micro-dissection punching system
title_sort	site-specific gene expression analysis using an automated tissue micro-dissection punching system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/64fa6cea83a0462089f492141d799655
work_keys_str_mv	AT takuyayoda sitespecificgeneexpressionanalysisusinganautomatedtissuemicrodissectionpunchingsystem AT masahitohosokawa sitespecificgeneexpressionanalysisusinganautomatedtissuemicrodissectionpunchingsystem AT kiyofumitakahashi sitespecificgeneexpressionanalysisusinganautomatedtissuemicrodissectionpunchingsystem AT chikakosakanashi sitespecificgeneexpressionanalysisusinganautomatedtissuemicrodissectionpunchingsystem AT harukotakeyama sitespecificgeneexpressionanalysisusinganautomatedtissuemicrodissectionpunchingsystem AT hidekikambara sitespecificgeneexpressionanalysisusinganautomatedtissuemicrodissectionpunchingsystem
_version_	1718395519140626432

Site-specific gene expression analysis using an automated tissue micro-dissection punching system

Ejemplares similares