Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)

Alpine habitats are shaped by harsh abiotic conditions and cold climates. Temperature stress can affect phenotypic plasticity, reproduction, and epigenetic profiles, which may affect acclimation and adaptation. Distribution patterns suggest that polyploidy seems to be advantageous under cold conditi...

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Autores principales:	Eleni Syngelaki, Claudia Paetzold, Elvira Hörandl
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	alpine plants cold stress DNA methylation gene expression Gene Set Enrichment Analysis geographical parthenogenesis Genetics QH426-470
Acceso en línea:	https://doaj.org/article/6508606736a643b38ab5f508d998b355
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spelling	oai:doaj.org-article:6508606736a643b38ab5f508d998b3552021-11-25T17:42:21ZGene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)10.3390/genes121118182073-4425https://doaj.org/article/6508606736a643b38ab5f508d998b3552021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1818https://doaj.org/toc/2073-4425Alpine habitats are shaped by harsh abiotic conditions and cold climates. Temperature stress can affect phenotypic plasticity, reproduction, and epigenetic profiles, which may affect acclimation and adaptation. Distribution patterns suggest that polyploidy seems to be advantageous under cold conditions. Nevertheless, whether temperature stress can induce gene expression changes in different cytotypes, and how the response is initialized through gene set pathways and epigenetic control remain vague for non-model plants. The perennial alpine plant <i>Ranunculus kuepferi</i> was used to investigate the effect of cold stress on gene expression profiles. Diploid and autotetraploid individuals were exposed to cold and warm conditions in climate growth chambers and analyzed via transcriptome sequencing and qRT-PCR. Overall, cold stress changed gene expression profiles of both cytotypes and induced cold acclimation. Diploids changed more gene set pathways than tetraploids, and suppressed pathways involved in ion/cation homeostasis. Tetraploids mostly activated gene set pathways related to cell wall and plasma membrane. An epigenetic background for gene regulation in response to temperature conditions is indicated. Results suggest that perennial alpine plants can respond to temperature extremes via altered gene expression. Tetraploids are better acclimated to cold conditions, enabling them to colonize colder climatic areas in the Alps.Eleni SyngelakiClaudia PaetzoldElvira HörandlMDPI AGarticlealpine plantscold stressDNA methylationgene expressionGene Set Enrichment Analysisgeographical parthenogenesisGeneticsQH426-470ENGenes, Vol 12, Iss 1818, p 1818 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alpine plants cold stress DNA methylation gene expression Gene Set Enrichment Analysis geographical parthenogenesis Genetics QH426-470
spellingShingle	alpine plants cold stress DNA methylation gene expression Gene Set Enrichment Analysis geographical parthenogenesis Genetics QH426-470 Eleni Syngelaki Claudia Paetzold Elvira Hörandl Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)
description	Alpine habitats are shaped by harsh abiotic conditions and cold climates. Temperature stress can affect phenotypic plasticity, reproduction, and epigenetic profiles, which may affect acclimation and adaptation. Distribution patterns suggest that polyploidy seems to be advantageous under cold conditions. Nevertheless, whether temperature stress can induce gene expression changes in different cytotypes, and how the response is initialized through gene set pathways and epigenetic control remain vague for non-model plants. The perennial alpine plant <i>Ranunculus kuepferi</i> was used to investigate the effect of cold stress on gene expression profiles. Diploid and autotetraploid individuals were exposed to cold and warm conditions in climate growth chambers and analyzed via transcriptome sequencing and qRT-PCR. Overall, cold stress changed gene expression profiles of both cytotypes and induced cold acclimation. Diploids changed more gene set pathways than tetraploids, and suppressed pathways involved in ion/cation homeostasis. Tetraploids mostly activated gene set pathways related to cell wall and plasma membrane. An epigenetic background for gene regulation in response to temperature conditions is indicated. Results suggest that perennial alpine plants can respond to temperature extremes via altered gene expression. Tetraploids are better acclimated to cold conditions, enabling them to colonize colder climatic areas in the Alps.
format	article
author	Eleni Syngelaki Claudia Paetzold Elvira Hörandl
author_facet	Eleni Syngelaki Claudia Paetzold Elvira Hörandl
author_sort	Eleni Syngelaki
title	Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)
title_short	Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)
title_full	Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)
title_fullStr	Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)
title_full_unstemmed	Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)
title_sort	gene expression profiles suggest a better cold acclimation of polyploids in the alpine species <i>ranunculus kuepferi</i> (ranunculaceae)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6508606736a643b38ab5f508d998b355
work_keys_str_mv	AT elenisyngelaki geneexpressionprofilessuggestabettercoldacclimationofpolyploidsinthealpinespeciesiranunculuskuepferiiranunculaceae AT claudiapaetzold geneexpressionprofilessuggestabettercoldacclimationofpolyploidsinthealpinespeciesiranunculuskuepferiiranunculaceae AT elvirahorandl geneexpressionprofilessuggestabettercoldacclimationofpolyploidsinthealpinespeciesiranunculuskuepferiiranunculaceae
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Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species <i>Ranunculus kuepferi</i> (Ranunculaceae)

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