Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray

Abstract There is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. Discovery of novel anti-adhesive molecules from natural product libraries will require the use of a high throughput screening platf...

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Autores principales:	Marta Utratna, Heidi Annuk, Jared Q. Gerlach, Yuan C. Lee, Marian Kane, Michelle Kilcoyne, Lokesh Joshi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6508bce32d4b4e14b2c19125fdd3ff0a
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spelling	oai:doaj.org-article:6508bce32d4b4e14b2c19125fdd3ff0a2021-12-02T11:53:05ZRapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray10.1038/s41598-017-06797-62045-2322https://doaj.org/article/6508bce32d4b4e14b2c19125fdd3ff0a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06797-6https://doaj.org/toc/2045-2322Abstract There is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. Discovery of novel anti-adhesive molecules from natural product libraries will require the use of a high throughput screening platform. Avian egg white (EW) provides nutrition for the embryo and protects against infection, with glycosylation responsible for binding certain pathogens. In this study, a microarray platform of 78 species of avian EWs was developed and profiled for glycosylation using a lectin panel with a wide range of carbohydrate specificities. The dominating linkages of sialic acid in EWs were determined for the first time using the lectins MAA and SNA-I. EW glycosylation similarity among the different orders of birds did not strictly depend on phylogenetic relationship. The interactions of five strains of bacterial pathogens, including Escherichia coli, Staphylococcus aureus and Vibrio cholera, identified a number of EWs as potential anti-adhesives, with some as strain- or species-specific. Of the two bacterial toxins examined, shiga-like toxin 1 subunit B bound to ten EWs with similar glycosylation more intensely than pigeon EW. This study provides a unique platform for high throughput screening of natural products for specific glycosylation and pathogen interactions. This platform may provide a useful platform in the future for discovery of anti-adhesives targeted for strain and species specificity.Marta UtratnaHeidi AnnukJared Q. GerlachYuan C. LeeMarian KaneMichelle KilcoyneLokesh JoshiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Utratna Heidi Annuk Jared Q. Gerlach Yuan C. Lee Marian Kane Michelle Kilcoyne Lokesh Joshi Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
description	Abstract There is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. Discovery of novel anti-adhesive molecules from natural product libraries will require the use of a high throughput screening platform. Avian egg white (EW) provides nutrition for the embryo and protects against infection, with glycosylation responsible for binding certain pathogens. In this study, a microarray platform of 78 species of avian EWs was developed and profiled for glycosylation using a lectin panel with a wide range of carbohydrate specificities. The dominating linkages of sialic acid in EWs were determined for the first time using the lectins MAA and SNA-I. EW glycosylation similarity among the different orders of birds did not strictly depend on phylogenetic relationship. The interactions of five strains of bacterial pathogens, including Escherichia coli, Staphylococcus aureus and Vibrio cholera, identified a number of EWs as potential anti-adhesives, with some as strain- or species-specific. Of the two bacterial toxins examined, shiga-like toxin 1 subunit B bound to ten EWs with similar glycosylation more intensely than pigeon EW. This study provides a unique platform for high throughput screening of natural products for specific glycosylation and pathogen interactions. This platform may provide a useful platform in the future for discovery of anti-adhesives targeted for strain and species specificity.
format	article
author	Marta Utratna Heidi Annuk Jared Q. Gerlach Yuan C. Lee Marian Kane Michelle Kilcoyne Lokesh Joshi
author_facet	Marta Utratna Heidi Annuk Jared Q. Gerlach Yuan C. Lee Marian Kane Michelle Kilcoyne Lokesh Joshi
author_sort	Marta Utratna
title	Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
title_short	Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
title_full	Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
title_fullStr	Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
title_full_unstemmed	Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
title_sort	rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6508bce32d4b4e14b2c19125fdd3ff0a
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Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray

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