Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1

Gut microbiota have myriad roles in host physiology, development, and immunity. Though confined to the intestinal lumen by the epithelia, microbes influence distal systems via poorly characterized mechanisms. Recent work has considered the role of extracellular vesicles in interspecies communication...

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Autores principales:	Kevin Champagne-Jorgensen, Tamina A. Jose, Andrew M. Stanisz, M. Firoz Mian, Alexander P. Hynes, John Bienenstock
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	bacteriophage commensal extracellular vesicles interleukin 10 lactobacillus microbiome microbiota-gut-brain axis microvesicle probiotic tlr2 Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/650981570e584d369154d625f86e52bc
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spelling	oai:doaj.org-article:650981570e584d369154d625f86e52bc2021-11-26T11:19:48ZBacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-11949-09761949-098410.1080/19490976.2021.1993583https://doaj.org/article/650981570e584d369154d625f86e52bc2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19490976.2021.1993583https://doaj.org/toc/1949-0976https://doaj.org/toc/1949-0984Gut microbiota have myriad roles in host physiology, development, and immunity. Though confined to the intestinal lumen by the epithelia, microbes influence distal systems via poorly characterized mechanisms. Recent work has considered the role of extracellular vesicles in interspecies communication, but whether they are involved in systemic microbe-host interaction is unclear. Here, we show that distinctive nanoparticles can be isolated from mouse blood within 2.5 h of consuming Lacticaseibacillus rhamnosus JB-1. In contrast to blood nanoparticles from saline-fed mice, they reproduced lipoteichoic acid-mediated immune functions of the original bacteria, including activation of TLR2 and increased IL-10 expression by dendritic cells. Like the fed bacteria, they also reduced IL-8 induced by TNF in an intestinal epithelial cell line. Though enriched for host neuronal proteins, these isolated nanoparticles also contained proteins and viral (phage) DNA of fed bacterial origin. Our data strongly suggest that oral consumption of live bacteria rapidly leads to circulation of their membrane vesicles and phages and demonstrate a nanoparticulate pathway whereby beneficial bacteria and probiotics may systemically affect their hosts.Kevin Champagne-JorgensenTamina A. JoseAndrew M. StaniszM. Firoz MianAlexander P. HynesJohn BienenstockTaylor & Francis Grouparticlebacteriophagecommensalextracellular vesiclesinterleukin 10lactobacillusmicrobiomemicrobiota-gut-brain axismicrovesicleprobiotictlr2Diseases of the digestive system. GastroenterologyRC799-869ENGut Microbes, Vol 13, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriophage commensal extracellular vesicles interleukin 10 lactobacillus microbiome microbiota-gut-brain axis microvesicle probiotic tlr2 Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	bacteriophage commensal extracellular vesicles interleukin 10 lactobacillus microbiome microbiota-gut-brain axis microvesicle probiotic tlr2 Diseases of the digestive system. Gastroenterology RC799-869 Kevin Champagne-Jorgensen Tamina A. Jose Andrew M. Stanisz M. Firoz Mian Alexander P. Hynes John Bienenstock Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1
description	Gut microbiota have myriad roles in host physiology, development, and immunity. Though confined to the intestinal lumen by the epithelia, microbes influence distal systems via poorly characterized mechanisms. Recent work has considered the role of extracellular vesicles in interspecies communication, but whether they are involved in systemic microbe-host interaction is unclear. Here, we show that distinctive nanoparticles can be isolated from mouse blood within 2.5 h of consuming Lacticaseibacillus rhamnosus JB-1. In contrast to blood nanoparticles from saline-fed mice, they reproduced lipoteichoic acid-mediated immune functions of the original bacteria, including activation of TLR2 and increased IL-10 expression by dendritic cells. Like the fed bacteria, they also reduced IL-8 induced by TNF in an intestinal epithelial cell line. Though enriched for host neuronal proteins, these isolated nanoparticles also contained proteins and viral (phage) DNA of fed bacterial origin. Our data strongly suggest that oral consumption of live bacteria rapidly leads to circulation of their membrane vesicles and phages and demonstrate a nanoparticulate pathway whereby beneficial bacteria and probiotics may systemically affect their hosts.
format	article
author	Kevin Champagne-Jorgensen Tamina A. Jose Andrew M. Stanisz M. Firoz Mian Alexander P. Hynes John Bienenstock
author_facet	Kevin Champagne-Jorgensen Tamina A. Jose Andrew M. Stanisz M. Firoz Mian Alexander P. Hynes John Bienenstock
author_sort	Kevin Champagne-Jorgensen
title	Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1
title_short	Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1
title_full	Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1
title_fullStr	Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1
title_full_unstemmed	Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1
title_sort	bacterial membrane vesicles and phages in blood after consumption of lacticaseibacillus rhamnosus jb-1
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/650981570e584d369154d625f86e52bc
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Bacterial membrane vesicles and phages in blood after consumption of Lacticaseibacillus rhamnosus JB-1

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