Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota

Abstract Human microbiota-associated (HMA) mice are an important model to study the relationship between liver diseases and intestinal microbiota. We describe a new method to humanize conventional mice based on bowel cleansing with polyethylene glycol followed by fecal microbiota transplantation (FM...

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Autores principales:	Laura Wrzosek, Dragos Ciocan, Patrick Borentain, Madeleine Spatz, Virginie Puchois, Cindy Hugot, Gladys Ferrere, Camille Mayeur, Gabriel Perlemuter, Anne-Marie Cassard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/650da944d3904ca0b9d84f077e204850
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spelling	oai:doaj.org-article:650da944d3904ca0b9d84f077e2048502021-12-02T16:08:02ZTransplantation of human microbiota into conventional mice durably reshapes the gut microbiota10.1038/s41598-018-25300-32045-2322https://doaj.org/article/650da944d3904ca0b9d84f077e2048502018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25300-3https://doaj.org/toc/2045-2322Abstract Human microbiota-associated (HMA) mice are an important model to study the relationship between liver diseases and intestinal microbiota. We describe a new method to humanize conventional mice based on bowel cleansing with polyethylene glycol followed by fecal microbiota transplantation (FMT) from a human donor. Four successive bowel cleansings were sufficient to empty the intestine and decrease the microbiota by 90%. We then compared four different strategies based on the frequency of FMT over four weeks: (1) twice a week; (2) once a week; (3) two FMTs; (4) one FMT. We were able to transfer human bacteria to mice, irrespective of the strategy used. We detected human bacteria after four weeks, even if only one FMT was performed, but there was a shift of the microbiota over time. FMT twice a week for four weeks was too frequent and perturbed the stability of the newly formed ecosystem. FMT once a week appears to be the best compromise as it allowed engraftment of Faecalibacterium, and a higher diversity of bacteria belonging to the Bacteroidales order. Our easy to establish HMA mouse model could be used as an alternative to classical HMA mice to study the relationship between the liver and the microbiota.Laura WrzosekDragos CiocanPatrick BorentainMadeleine SpatzVirginie PuchoisCindy HugotGladys FerrereCamille MayeurGabriel PerlemuterAnne-Marie CassardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Wrzosek Dragos Ciocan Patrick Borentain Madeleine Spatz Virginie Puchois Cindy Hugot Gladys Ferrere Camille Mayeur Gabriel Perlemuter Anne-Marie Cassard Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
description	Abstract Human microbiota-associated (HMA) mice are an important model to study the relationship between liver diseases and intestinal microbiota. We describe a new method to humanize conventional mice based on bowel cleansing with polyethylene glycol followed by fecal microbiota transplantation (FMT) from a human donor. Four successive bowel cleansings were sufficient to empty the intestine and decrease the microbiota by 90%. We then compared four different strategies based on the frequency of FMT over four weeks: (1) twice a week; (2) once a week; (3) two FMTs; (4) one FMT. We were able to transfer human bacteria to mice, irrespective of the strategy used. We detected human bacteria after four weeks, even if only one FMT was performed, but there was a shift of the microbiota over time. FMT twice a week for four weeks was too frequent and perturbed the stability of the newly formed ecosystem. FMT once a week appears to be the best compromise as it allowed engraftment of Faecalibacterium, and a higher diversity of bacteria belonging to the Bacteroidales order. Our easy to establish HMA mouse model could be used as an alternative to classical HMA mice to study the relationship between the liver and the microbiota.
format	article
author	Laura Wrzosek Dragos Ciocan Patrick Borentain Madeleine Spatz Virginie Puchois Cindy Hugot Gladys Ferrere Camille Mayeur Gabriel Perlemuter Anne-Marie Cassard
author_facet	Laura Wrzosek Dragos Ciocan Patrick Borentain Madeleine Spatz Virginie Puchois Cindy Hugot Gladys Ferrere Camille Mayeur Gabriel Perlemuter Anne-Marie Cassard
author_sort	Laura Wrzosek
title	Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
title_short	Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
title_full	Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
title_fullStr	Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
title_full_unstemmed	Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
title_sort	transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/650da944d3904ca0b9d84f077e204850
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Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota

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