Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.

We described a quorum-sensing mechanism in the streptococci genus involving a short hydrophobic peptide (SHP), which acts as a pheromone, and a transcriptional regulator belonging to the Rgg family. The shp/rgg genes, found in nearly all streptococcal genomes and in several copies in some, have been...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Betty Fleuchot, Alain Guillot, Christine Mézange, Colette Besset, Emilie Chambellon, Véronique Monnet, Rozenn Gardan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65125659154f4d83bfac47e0660f1eba
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:65125659154f4d83bfac47e0660f1eba
record_format	dspace
spelling	oai:doaj.org-article:65125659154f4d83bfac47e0660f1eba2021-11-18T07:42:16ZRgg-associated SHP signaling peptides mediate cross-talk in Streptococci.1932-620310.1371/journal.pone.0066042https://doaj.org/article/65125659154f4d83bfac47e0660f1eba2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23776602/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We described a quorum-sensing mechanism in the streptococci genus involving a short hydrophobic peptide (SHP), which acts as a pheromone, and a transcriptional regulator belonging to the Rgg family. The shp/rgg genes, found in nearly all streptococcal genomes and in several copies in some, have been classified into three groups. We used a genetic approach to evaluate the functionality of the SHP/Rgg quorum-sensing mechanism, encoded by three selected shp/rgg loci, in pathogenic and non-pathogenic streptococci. We characterized the mature form of each SHP pheromone by mass-spectrometry. We produced synthetic peptides corresponding to these mature forms, and used them to study functional complementation and cross-talk between these different SHP/Rgg systems. We demonstrate that a SHP pheromone of one system can influence the activity of a different system. Interestingly, this does not seem to be dependent on the SHP/Rgg group and cross-talk between pathogenic and non-pathogenic streptococci is observed.Betty FleuchotAlain GuillotChristine MézangeColette BessetEmilie ChambellonVéronique MonnetRozenn GardanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66042 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Betty Fleuchot Alain Guillot Christine Mézange Colette Besset Emilie Chambellon Véronique Monnet Rozenn Gardan Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.
description	We described a quorum-sensing mechanism in the streptococci genus involving a short hydrophobic peptide (SHP), which acts as a pheromone, and a transcriptional regulator belonging to the Rgg family. The shp/rgg genes, found in nearly all streptococcal genomes and in several copies in some, have been classified into three groups. We used a genetic approach to evaluate the functionality of the SHP/Rgg quorum-sensing mechanism, encoded by three selected shp/rgg loci, in pathogenic and non-pathogenic streptococci. We characterized the mature form of each SHP pheromone by mass-spectrometry. We produced synthetic peptides corresponding to these mature forms, and used them to study functional complementation and cross-talk between these different SHP/Rgg systems. We demonstrate that a SHP pheromone of one system can influence the activity of a different system. Interestingly, this does not seem to be dependent on the SHP/Rgg group and cross-talk between pathogenic and non-pathogenic streptococci is observed.
format	article
author	Betty Fleuchot Alain Guillot Christine Mézange Colette Besset Emilie Chambellon Véronique Monnet Rozenn Gardan
author_facet	Betty Fleuchot Alain Guillot Christine Mézange Colette Besset Emilie Chambellon Véronique Monnet Rozenn Gardan
author_sort	Betty Fleuchot
title	Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.
title_short	Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.
title_full	Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.
title_fullStr	Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.
title_full_unstemmed	Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.
title_sort	rgg-associated shp signaling peptides mediate cross-talk in streptococci.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/65125659154f4d83bfac47e0660f1eba
work_keys_str_mv	AT bettyfleuchot rggassociatedshpsignalingpeptidesmediatecrosstalkinstreptococci AT alainguillot rggassociatedshpsignalingpeptidesmediatecrosstalkinstreptococci AT christinemezange rggassociatedshpsignalingpeptidesmediatecrosstalkinstreptococci AT colettebesset rggassociatedshpsignalingpeptidesmediatecrosstalkinstreptococci AT emiliechambellon rggassociatedshpsignalingpeptidesmediatecrosstalkinstreptococci AT veroniquemonnet rggassociatedshpsignalingpeptidesmediatecrosstalkinstreptococci AT rozenngardan rggassociatedshpsignalingpeptidesmediatecrosstalkinstreptococci
_version_	1718423099950497792

Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.

Ejemplares similares