Transportation container for pre-processing cytogenetic assays in radiation accidents

Abstract We report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jian Gu, Brett Duane, Mikhail Repin, David J. Brenner, Frederic Zenhausern
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6529385aa13241258b3994041104e94f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6529385aa13241258b3994041104e94f
record_format	dspace
spelling	oai:doaj.org-article:6529385aa13241258b3994041104e94f2021-12-02T14:58:45ZTransportation container for pre-processing cytogenetic assays in radiation accidents10.1038/s41598-021-89832-x2045-2322https://doaj.org/article/6529385aa13241258b3994041104e94f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89832-xhttps://doaj.org/toc/2045-2322Abstract We report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 °C) to meet the biodosimetry assay requirements. Using an existing insulated shipping box and long shelf life alkaline batteries makes it ideal for national stockpile. Dose curve of cytogenetic biodosimetry assay using the shipping container showed clear dose response and high linear correlation with the control dose curve using a laboratory incubator (Pearson’s correlation coefficient: 0.992). The container’s ability of pre-processing biological samples during transportation could have a significant impact on radiation countermeasure, as well as potential impacts in other applications such as biobanking, novel molecular or cell-based assays or therapies.Jian GuBrett DuaneMikhail RepinDavid J. BrennerFrederic ZenhausernNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jian Gu Brett Duane Mikhail Repin David J. Brenner Frederic Zenhausern Transportation container for pre-processing cytogenetic assays in radiation accidents
description	Abstract We report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 °C) to meet the biodosimetry assay requirements. Using an existing insulated shipping box and long shelf life alkaline batteries makes it ideal for national stockpile. Dose curve of cytogenetic biodosimetry assay using the shipping container showed clear dose response and high linear correlation with the control dose curve using a laboratory incubator (Pearson’s correlation coefficient: 0.992). The container’s ability of pre-processing biological samples during transportation could have a significant impact on radiation countermeasure, as well as potential impacts in other applications such as biobanking, novel molecular or cell-based assays or therapies.
format	article
author	Jian Gu Brett Duane Mikhail Repin David J. Brenner Frederic Zenhausern
author_facet	Jian Gu Brett Duane Mikhail Repin David J. Brenner Frederic Zenhausern
author_sort	Jian Gu
title	Transportation container for pre-processing cytogenetic assays in radiation accidents
title_short	Transportation container for pre-processing cytogenetic assays in radiation accidents
title_full	Transportation container for pre-processing cytogenetic assays in radiation accidents
title_fullStr	Transportation container for pre-processing cytogenetic assays in radiation accidents
title_full_unstemmed	Transportation container for pre-processing cytogenetic assays in radiation accidents
title_sort	transportation container for pre-processing cytogenetic assays in radiation accidents
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6529385aa13241258b3994041104e94f
work_keys_str_mv	AT jiangu transportationcontainerforpreprocessingcytogeneticassaysinradiationaccidents AT brettduane transportationcontainerforpreprocessingcytogeneticassaysinradiationaccidents AT mikhailrepin transportationcontainerforpreprocessingcytogeneticassaysinradiationaccidents AT davidjbrenner transportationcontainerforpreprocessingcytogeneticassaysinradiationaccidents AT fredericzenhausern transportationcontainerforpreprocessingcytogeneticassaysinradiationaccidents
_version_	1718389269066678272

Transportation container for pre-processing cytogenetic assays in radiation accidents

Ejemplares similares