3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus

Alexander M Demin,1,* Alexandra G Pershina,2,3,* Vladimir V Ivanov,2 Kseniya V Nevskaya,2 Oleg B Shevelev,4 Artyom S Minin,5 Iliya V Byzov,5 Alexey E Sazonov,2 Victor P Krasnov,1 Ludmila M Ogorodova2 1Postovsky Institute of Organic Synthesis of RAS (Ural Branch), Yekaterinburg, 2Siberian State Medi...

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Autores principales:	Demin AM, Pershina AG, Ivanov VV, Nevskaya KV, Shevelev OB, Minin AS, Byzov IV, Sazonov AE, Krasnov VP, Ogorodova LM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	magnetic nanoparticles alkoxysilane magnetic resonance imaging liver fluke Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6529bd0c47434861940300b3d749a1a3
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spelling	oai:doaj.org-article:6529bd0c47434861940300b3d749a1a32021-12-02T03:11:48Z3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus1178-2013https://doaj.org/article/6529bd0c47434861940300b3d749a1a32016-09-01T00:00:00Zhttps://www.dovepress.com/3-aminopropylsilane-modified-iron-oxide-nanoparticles-for-contrast-enh-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Alexander M Demin,1,* Alexandra G Pershina,2,3,* Vladimir V Ivanov,2 Kseniya V Nevskaya,2 Oleg B Shevelev,4 Artyom S Minin,5 Iliya V Byzov,5 Alexey E Sazonov,2 Victor P Krasnov,1 Ludmila M Ogorodova2 1Postovsky Institute of Organic Synthesis of RAS (Ural Branch), Yekaterinburg, 2Siberian State Medical University, 3Russian National Research Tomsk Polytechnic University, Tomsk, 4Institute of Cytology and Genetics SB RAS, Novosibirsk, 5Miheev Institute of Metal Physics of RAS (Ural Branch), Yekaterinburg, Russia *These authors contributed equally to this work Purpose: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites.Materials and methods: Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe3+ and Fe2+ salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis . Magnetic resonance properties of MNPs were investigated in vitro and in vivo.Results: The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g-1) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r2 was 271 mmol-1 s-1. To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus.Conclusion: The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage. Keywords: magnetic nanoparticles, alkoxysilane, magnetic resonance imaging, liver flukeDemin AMPershina AGIvanov VVNevskaya KVShevelev OBMinin ASByzov IVSazonov AEKrasnov VPOgorodova LMDove Medical Pressarticlemagnetic nanoparticlesalkoxysilanemagnetic resonance imagingliver flukeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4451-4463 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetic nanoparticles alkoxysilane magnetic resonance imaging liver fluke Medicine (General) R5-920
spellingShingle	magnetic nanoparticles alkoxysilane magnetic resonance imaging liver fluke Medicine (General) R5-920 Demin AM Pershina AG Ivanov VV Nevskaya KV Shevelev OB Minin AS Byzov IV Sazonov AE Krasnov VP Ogorodova LM 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
description	Alexander M Demin,1,* Alexandra G Pershina,2,3,* Vladimir V Ivanov,2 Kseniya V Nevskaya,2 Oleg B Shevelev,4 Artyom S Minin,5 Iliya V Byzov,5 Alexey E Sazonov,2 Victor P Krasnov,1 Ludmila M Ogorodova2 1Postovsky Institute of Organic Synthesis of RAS (Ural Branch), Yekaterinburg, 2Siberian State Medical University, 3Russian National Research Tomsk Polytechnic University, Tomsk, 4Institute of Cytology and Genetics SB RAS, Novosibirsk, 5Miheev Institute of Metal Physics of RAS (Ural Branch), Yekaterinburg, Russia *These authors contributed equally to this work Purpose: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites.Materials and methods: Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe3+ and Fe2+ salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis . Magnetic resonance properties of MNPs were investigated in vitro and in vivo.Results: The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g-1) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r2 was 271 mmol-1 s-1. To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus.Conclusion: The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage. Keywords: magnetic nanoparticles, alkoxysilane, magnetic resonance imaging, liver fluke
format	article
author	Demin AM Pershina AG Ivanov VV Nevskaya KV Shevelev OB Minin AS Byzov IV Sazonov AE Krasnov VP Ogorodova LM
author_facet	Demin AM Pershina AG Ivanov VV Nevskaya KV Shevelev OB Minin AS Byzov IV Sazonov AE Krasnov VP Ogorodova LM
author_sort	Demin AM
title	3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
title_short	3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
title_full	3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
title_fullStr	3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
title_full_unstemmed	3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
title_sort	3-aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by opisthorchis felineus
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/6529bd0c47434861940300b3d749a1a3
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3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus

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