The physiological and molecular response of Aurelia sp.1 under hypoxia

Abstract Few studies have been published on the mechanisms of hypoxia response and tolerance in jellyfish, especially with respect to the regulatory mechanism at the molecular level. In this study, Aurelia sp.1, which is frequently found in Chinese coastal waters, was cultivated in a hypoxic system...

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Autores principales:	Guoshan Wang, Yu Zhen, Zhigang Yu, Yan Shi, Qing Zhao, Jianyan Wang, Tiezhu Mi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/652a51eccb494d08bdc32c8797e1b4f5
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spelling	oai:doaj.org-article:652a51eccb494d08bdc32c8797e1b4f52021-12-02T11:52:42ZThe physiological and molecular response of Aurelia sp.1 under hypoxia10.1038/s41598-017-01318-x2045-2322https://doaj.org/article/652a51eccb494d08bdc32c8797e1b4f52017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01318-xhttps://doaj.org/toc/2045-2322Abstract Few studies have been published on the mechanisms of hypoxia response and tolerance in jellyfish, especially with respect to the regulatory mechanism at the molecular level. In this study, Aurelia sp.1, which is frequently found in Chinese coastal waters, was cultivated in a hypoxic system to determine the molecular mechanisms underlying its hypoxic response by studying the physiological activity, gene expression and metabolite contents in the prolyl hydroxylase domain (PHD)-hypoxia inducible factor (HIF) oxygen-sensing system. Physiological activity; the expression of PHD, HIF, ALDO (fructose-bisphosphate aldolase), PDK (pyruvate dehydrogenase kinase), and LDH (lactate dehydrogenase) genes; and the lactic acid content in medusae were significantly affected by hypoxia. The up-regulation of ALDO, PDK and LDH, which was directly or indirectly induced by HIF, mediated the transition from aerobic respiration to anaerobic glycolysis in the medusae. In polyps, there was a slight increase in the expression of HIF, PHD and ALDO, no obvious change in that of PDK and a slight decrease in that of LDH throughout the experiment; however, these changes were insufficient to induce the shift. This study provides a scientific basis for elucidating the regulatory mechanism underlying the PHD-HIF oxygen-sensing system in Aurelia sp.1.Guoshan WangYu ZhenZhigang YuYan ShiQing ZhaoJianyan WangTiezhu MiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guoshan Wang Yu Zhen Zhigang Yu Yan Shi Qing Zhao Jianyan Wang Tiezhu Mi The physiological and molecular response of Aurelia sp.1 under hypoxia
description	Abstract Few studies have been published on the mechanisms of hypoxia response and tolerance in jellyfish, especially with respect to the regulatory mechanism at the molecular level. In this study, Aurelia sp.1, which is frequently found in Chinese coastal waters, was cultivated in a hypoxic system to determine the molecular mechanisms underlying its hypoxic response by studying the physiological activity, gene expression and metabolite contents in the prolyl hydroxylase domain (PHD)-hypoxia inducible factor (HIF) oxygen-sensing system. Physiological activity; the expression of PHD, HIF, ALDO (fructose-bisphosphate aldolase), PDK (pyruvate dehydrogenase kinase), and LDH (lactate dehydrogenase) genes; and the lactic acid content in medusae were significantly affected by hypoxia. The up-regulation of ALDO, PDK and LDH, which was directly or indirectly induced by HIF, mediated the transition from aerobic respiration to anaerobic glycolysis in the medusae. In polyps, there was a slight increase in the expression of HIF, PHD and ALDO, no obvious change in that of PDK and a slight decrease in that of LDH throughout the experiment; however, these changes were insufficient to induce the shift. This study provides a scientific basis for elucidating the regulatory mechanism underlying the PHD-HIF oxygen-sensing system in Aurelia sp.1.
format	article
author	Guoshan Wang Yu Zhen Zhigang Yu Yan Shi Qing Zhao Jianyan Wang Tiezhu Mi
author_facet	Guoshan Wang Yu Zhen Zhigang Yu Yan Shi Qing Zhao Jianyan Wang Tiezhu Mi
author_sort	Guoshan Wang
title	The physiological and molecular response of Aurelia sp.1 under hypoxia
title_short	The physiological and molecular response of Aurelia sp.1 under hypoxia
title_full	The physiological and molecular response of Aurelia sp.1 under hypoxia
title_fullStr	The physiological and molecular response of Aurelia sp.1 under hypoxia
title_full_unstemmed	The physiological and molecular response of Aurelia sp.1 under hypoxia
title_sort	physiological and molecular response of aurelia sp.1 under hypoxia
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/652a51eccb494d08bdc32c8797e1b4f5
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The physiological and molecular response of Aurelia sp.1 under hypoxia

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