Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters

ProAKAP4 is synthetized as a precursor polypeptide that must be converted into mature AKAP4 in living spermatozoa and is considered as a functional marker of spermatozoa. The gene is well-conserved in mammals although uncharacterized in Camelidae. In the present study, we investigate the expression...

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Autores principales:	Clara Malo, Sara Carracedo, Maryse Delehedde, Nicolas Sergeant, Julian Alexandra Skidmore
Formato:	article
Lenguaje:	EN
Publicado:	Bioscientifica 2021
Materias:	dromedary camel proakap4 spermatozoa seasonality Reproduction QH471-489 Gynecology and obstetrics RG1-991
Acceso en línea:	https://doi.org/10.1530/RAF-21-0055 https://doaj.org/article/652daf396b224aab9b5437140930413e
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spelling	oai:doaj.org-article:652daf396b224aab9b5437140930413e2021-11-18T06:51:35ZIdentification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parametershttps://doi.org/10.1530/RAF-21-00552633-8386https://doaj.org/article/652daf396b224aab9b5437140930413e2021-11-01T00:00:00Zhttps://raf.bioscientifica.com/view/journals/raf/2/4/RAF-21-0055.xmlhttps://doaj.org/toc/2633-8386ProAKAP4 is synthetized as a precursor polypeptide that must be converted into mature AKAP4 in living spermatozoa and is considered as a functional marker of spermatozoa. The gene is well-conserved in mammals although uncharacterized in Camelidae. In the present study, we investigate the expression metabolism of proAKAP4 and AKAP4 proteins and evaluate their seasonal dynamics relative to semen quality in dromedary camels. Semen parameters including volume and viscosity and characteristics of sperm including concentration, total production, total and progressive motility, vitality, acrosome integrity and morphological abnormalities were assessed in semen samples collected weekly from six camels during the rutting season, from November to April. Only total sperm production varied, peaking in January. Both the precursor proAKAP4 and AKAP4 proteins were investigated and shown to express biochemical properties similar to those described in other mammals. ProAKAP4 concentrations expressed in ng/10 million spermatozoa as assayed using a specific ELISA showed a strong positive correlation with ejaculate volume (P = 0.045), viscosity (P < 0.001) and sperm total motility (P = 0.049). Furthermore, their concentrations exhibited clear seasonal variations in camel semen. In conclusion, the assessment of proAKAP4 concentrations in camel sperm provides a novel parameter to assess sperm quality. Further studies should be performed to investigate proAKAP4 concentrations relative to fertility in Camelidae that may help to define the right time for mating and semen collection and increase the success of breeding programs.Clara MaloSara CarracedoMaryse DeleheddeNicolas SergeantJulian Alexandra SkidmoreBioscientificaarticledromedary camelproakap4spermatozoaseasonalityReproductionQH471-489Gynecology and obstetricsRG1-991ENReproduction and Fertility, Vol 2, Iss 4, Pp 268-279 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dromedary camel proakap4 spermatozoa seasonality Reproduction QH471-489 Gynecology and obstetrics RG1-991
spellingShingle	dromedary camel proakap4 spermatozoa seasonality Reproduction QH471-489 Gynecology and obstetrics RG1-991 Clara Malo Sara Carracedo Maryse Delehedde Nicolas Sergeant Julian Alexandra Skidmore Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters
description	ProAKAP4 is synthetized as a precursor polypeptide that must be converted into mature AKAP4 in living spermatozoa and is considered as a functional marker of spermatozoa. The gene is well-conserved in mammals although uncharacterized in Camelidae. In the present study, we investigate the expression metabolism of proAKAP4 and AKAP4 proteins and evaluate their seasonal dynamics relative to semen quality in dromedary camels. Semen parameters including volume and viscosity and characteristics of sperm including concentration, total production, total and progressive motility, vitality, acrosome integrity and morphological abnormalities were assessed in semen samples collected weekly from six camels during the rutting season, from November to April. Only total sperm production varied, peaking in January. Both the precursor proAKAP4 and AKAP4 proteins were investigated and shown to express biochemical properties similar to those described in other mammals. ProAKAP4 concentrations expressed in ng/10 million spermatozoa as assayed using a specific ELISA showed a strong positive correlation with ejaculate volume (P = 0.045), viscosity (P < 0.001) and sperm total motility (P = 0.049). Furthermore, their concentrations exhibited clear seasonal variations in camel semen. In conclusion, the assessment of proAKAP4 concentrations in camel sperm provides a novel parameter to assess sperm quality. Further studies should be performed to investigate proAKAP4 concentrations relative to fertility in Camelidae that may help to define the right time for mating and semen collection and increase the success of breeding programs.
format	article
author	Clara Malo Sara Carracedo Maryse Delehedde Nicolas Sergeant Julian Alexandra Skidmore
author_facet	Clara Malo Sara Carracedo Maryse Delehedde Nicolas Sergeant Julian Alexandra Skidmore
author_sort	Clara Malo
title	Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters
title_short	Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters
title_full	Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters
title_fullStr	Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters
title_full_unstemmed	Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters
title_sort	identification of proakap4 concentration variations in dromedary sperm and their correlation with monthly semen parameters
publisher	Bioscientifica
publishDate	2021
url	https://doi.org/10.1530/RAF-21-0055 https://doaj.org/article/652daf396b224aab9b5437140930413e
work_keys_str_mv	AT claramalo identificationofproakap4concentrationvariationsindromedaryspermandtheircorrelationwithmonthlysemenparameters AT saracarracedo identificationofproakap4concentrationvariationsindromedaryspermandtheircorrelationwithmonthlysemenparameters AT marysedelehedde identificationofproakap4concentrationvariationsindromedaryspermandtheircorrelationwithmonthlysemenparameters AT nicolassergeant identificationofproakap4concentrationvariationsindromedaryspermandtheircorrelationwithmonthlysemenparameters AT julianalexandraskidmore identificationofproakap4concentrationvariationsindromedaryspermandtheircorrelationwithmonthlysemenparameters
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Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters

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