Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.

<h4>Aim</h4>Diabetic foot ulcer (DFU) is a major concern in diabetes and its control requires in-depth molecular investigation. The present study aimed to screen the expression of microRNA-210 (miR-210) and its association in hypoxic pathway in DFU patients.<h4>Methods</h4>Th...

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Autores principales:	Sivakamasundari Pichu, Selvaraj Vimalraj, Vijay Viswanathan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/653324170ee04bdbbf62cb9397f1994b
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spelling	oai:doaj.org-article:653324170ee04bdbbf62cb9397f1994b2021-12-02T20:06:34ZImpact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.1932-620310.1371/journal.pone.0254921https://doaj.org/article/653324170ee04bdbbf62cb9397f1994b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254921https://doaj.org/toc/1932-6203<h4>Aim</h4>Diabetic foot ulcer (DFU) is a major concern in diabetes and its control requires in-depth molecular investigation. The present study aimed to screen the expression of microRNA-210 (miR-210) and its association in hypoxic pathway in DFU patients.<h4>Methods</h4>The study consists of 3 groups of circulation samples (50 in each group of: healthy volunteers, T2DM and T2DM with DFU) and 2 groups of tissue samples (10 in each group of: control and T2DM with DFU). Expression of miR-210 and hypoxia inducible factor-1 alpha (HIF-1α), and its responsive genes such as VEGF, TNF-α, IL-6, BCl2, Bax and Caspase 3 were analyzed by RT-PCR, Western blot and ELISA analyses.<h4>Results</h4>The HIF-1α expression decreased in DFU patients with increased miR-210 expression in both circulation and tissue biopsies. The circulatory IL-6 and inflammatory gene TNF-α expression was increased in DFU compared to healthy controls and T2DM subjects. Further, we found there was no alteration in the angiogenic marker, VEGF expression. In comparison, anti-apoptotic BCl2 was decreased and Bax and Caspase 3 was increased in DFU tissues relative to control.<h4>Conclusions</h4>The study showed that there was an inverse relationship between miR-210 and HIF-1α expression in patients with DFU, indicating that miR-210 may regulate the expression of the hypoxic gene.Sivakamasundari PichuSelvaraj VimalrajVijay ViswanathanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254921 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sivakamasundari Pichu Selvaraj Vimalraj Vijay Viswanathan Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.
description	<h4>Aim</h4>Diabetic foot ulcer (DFU) is a major concern in diabetes and its control requires in-depth molecular investigation. The present study aimed to screen the expression of microRNA-210 (miR-210) and its association in hypoxic pathway in DFU patients.<h4>Methods</h4>The study consists of 3 groups of circulation samples (50 in each group of: healthy volunteers, T2DM and T2DM with DFU) and 2 groups of tissue samples (10 in each group of: control and T2DM with DFU). Expression of miR-210 and hypoxia inducible factor-1 alpha (HIF-1α), and its responsive genes such as VEGF, TNF-α, IL-6, BCl2, Bax and Caspase 3 were analyzed by RT-PCR, Western blot and ELISA analyses.<h4>Results</h4>The HIF-1α expression decreased in DFU patients with increased miR-210 expression in both circulation and tissue biopsies. The circulatory IL-6 and inflammatory gene TNF-α expression was increased in DFU compared to healthy controls and T2DM subjects. Further, we found there was no alteration in the angiogenic marker, VEGF expression. In comparison, anti-apoptotic BCl2 was decreased and Bax and Caspase 3 was increased in DFU tissues relative to control.<h4>Conclusions</h4>The study showed that there was an inverse relationship between miR-210 and HIF-1α expression in patients with DFU, indicating that miR-210 may regulate the expression of the hypoxic gene.
format	article
author	Sivakamasundari Pichu Selvaraj Vimalraj Vijay Viswanathan
author_facet	Sivakamasundari Pichu Selvaraj Vimalraj Vijay Viswanathan
author_sort	Sivakamasundari Pichu
title	Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.
title_short	Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.
title_full	Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.
title_fullStr	Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.
title_full_unstemmed	Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.
title_sort	impact of microrna-210 on wound healing among the patients with diabetic foot ulcer.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/653324170ee04bdbbf62cb9397f1994b
work_keys_str_mv	AT sivakamasundaripichu impactofmicrorna210onwoundhealingamongthepatientswithdiabeticfootulcer AT selvarajvimalraj impactofmicrorna210onwoundhealingamongthepatientswithdiabeticfootulcer AT vijayviswanathan impactofmicrorna210onwoundhealingamongthepatientswithdiabeticfootulcer
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Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.

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