Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies

Assisted reproductive technologies (ART) are one of the most effective ways in fighting infertility, but their effectiveness is influenced by various factors. Our study focuses on examining importance of risk factors underlying ART failure related to altered microbiome pattern in the female reproduc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E A. Lebedeva, S. V. Rishchuk, T. A. Dushenkova, A. S. Mokhov, M. V. Desyatova, E. I. Ermolenko, G. F. Leontyeva, A. V. Svarval, E. E. Shchederkina, V. V. Kolodzhieva, L. Yu. Nilova, E. A. Orishak, A. E. Goncharov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	urogenital infections microbiome assisted reproductive technologies chlamydial infection trichomonas infection risk factors Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/6536274d7798462a8753bca36cd4a45f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6536274d7798462a8753bca36cd4a45f
record_format	dspace
spelling	oai:doaj.org-article:6536274d7798462a8753bca36cd4a45f2021-11-22T07:09:55ZAltered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies2220-76192313-739810.15789/2220-7619-CIT-1551https://doaj.org/article/6536274d7798462a8753bca36cd4a45f2021-03-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1551https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Assisted reproductive technologies (ART) are one of the most effective ways in fighting infertility, but their effectiveness is influenced by various factors. Our study focuses on examining importance of risk factors underlying ART failure related to altered microbiome pattern in the female reproductive system. The case-control study was based on conducting a laboratory examination of 129 infertile couples applied to the Department of Assisted Reproductive Technologies due to failure of in vitro fertilization (IVF), whereas control group consisted of females with successful progressive pregnancy after ART. Chlamydia, herpes virus and cytomegalovirus were assessed by using PCR and ELISA, whereas culture method was used to detect Trichomonas vaginalis in vaginal swabs and ejaculate applying Trichomonas Modified CPLM Medium (HiMedia, India) followed by microscopy. In addition, the qualitative and quantitative composition of the vaginal biocenosis was studied with multiplex RT-PCR by using Femoflor-16 kit (DNA-technologies, Russia). Serological tests were based on measuring IgG and IgA antibodies against Chlamydia trachomatis in ELISA (diagnostic kits purchased from Vektor Best, Russia; NovaTec, Germany; ImmunoComb, Israel) as well as antibodies against immediate early HSV-1/2 proteins (BioService, Russia) and immediate early HHV-5 proteins (Vector Best, Russia). C. trachomatis and herpesvirus DNA was measured by using PCR diagnostic kits Amplisens (Interlabservice, Russia). It was found that sexually transmitted infection agents were highly prevalent in infertile couples applying to the hospital for IVF. Significant factors for non-pregnancy were vaginal dysmicrobiocenosis (OR = 7.5 (95% CI 1.04—54.1), p = 0.02) and detected T. vaginalis (OR = 2.6 (95% CI 1.12—6.4), p = 0.01). Dysbiosis of the reproductive system, including those occurring due to trichomonas infection is associated with lowered ART effectiveness. It is evident to timely detect urogenital infections and dysbiosis while preparing infertile couples for IVF cycles.E A. LebedevaS. V. RishchukT. A. DushenkovaA. S. MokhovM. V. DesyatovaE. I. ErmolenkoG. F. LeontyevaA. V. SvarvalE. E. ShchederkinaV. V. KolodzhievaL. Yu. NilovaE. A. OrishakA. E. GoncharovSankt-Peterburg : NIIÈM imeni Pasteraarticleurogenital infectionsmicrobiomeassisted reproductive technologieschlamydial infectiontrichomonas infectionrisk factorsInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 2, Pp 365-370 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	urogenital infections microbiome assisted reproductive technologies chlamydial infection trichomonas infection risk factors Infectious and parasitic diseases RC109-216
spellingShingle	urogenital infections microbiome assisted reproductive technologies chlamydial infection trichomonas infection risk factors Infectious and parasitic diseases RC109-216 E A. Lebedeva S. V. Rishchuk T. A. Dushenkova A. S. Mokhov M. V. Desyatova E. I. Ermolenko G. F. Leontyeva A. V. Svarval E. E. Shchederkina V. V. Kolodzhieva L. Yu. Nilova E. A. Orishak A. E. Goncharov Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies
description	Assisted reproductive technologies (ART) are one of the most effective ways in fighting infertility, but their effectiveness is influenced by various factors. Our study focuses on examining importance of risk factors underlying ART failure related to altered microbiome pattern in the female reproductive system. The case-control study was based on conducting a laboratory examination of 129 infertile couples applied to the Department of Assisted Reproductive Technologies due to failure of in vitro fertilization (IVF), whereas control group consisted of females with successful progressive pregnancy after ART. Chlamydia, herpes virus and cytomegalovirus were assessed by using PCR and ELISA, whereas culture method was used to detect Trichomonas vaginalis in vaginal swabs and ejaculate applying Trichomonas Modified CPLM Medium (HiMedia, India) followed by microscopy. In addition, the qualitative and quantitative composition of the vaginal biocenosis was studied with multiplex RT-PCR by using Femoflor-16 kit (DNA-technologies, Russia). Serological tests were based on measuring IgG and IgA antibodies against Chlamydia trachomatis in ELISA (diagnostic kits purchased from Vektor Best, Russia; NovaTec, Germany; ImmunoComb, Israel) as well as antibodies against immediate early HSV-1/2 proteins (BioService, Russia) and immediate early HHV-5 proteins (Vector Best, Russia). C. trachomatis and herpesvirus DNA was measured by using PCR diagnostic kits Amplisens (Interlabservice, Russia). It was found that sexually transmitted infection agents were highly prevalent in infertile couples applying to the hospital for IVF. Significant factors for non-pregnancy were vaginal dysmicrobiocenosis (OR = 7.5 (95% CI 1.04—54.1), p = 0.02) and detected T. vaginalis (OR = 2.6 (95% CI 1.12—6.4), p = 0.01). Dysbiosis of the reproductive system, including those occurring due to trichomonas infection is associated with lowered ART effectiveness. It is evident to timely detect urogenital infections and dysbiosis while preparing infertile couples for IVF cycles.
format	article
author	E A. Lebedeva S. V. Rishchuk T. A. Dushenkova A. S. Mokhov M. V. Desyatova E. I. Ermolenko G. F. Leontyeva A. V. Svarval E. E. Shchederkina V. V. Kolodzhieva L. Yu. Nilova E. A. Orishak A. E. Goncharov
author_facet	E A. Lebedeva S. V. Rishchuk T. A. Dushenkova A. S. Mokhov M. V. Desyatova E. I. Ermolenko G. F. Leontyeva A. V. Svarval E. E. Shchederkina V. V. Kolodzhieva L. Yu. Nilova E. A. Orishak A. E. Goncharov
author_sort	E A. Lebedeva
title	Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies
title_short	Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies
title_full	Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies
title_fullStr	Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies
title_full_unstemmed	Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies
title_sort	altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/6536274d7798462a8753bca36cd4a45f
work_keys_str_mv	AT ealebedeva alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT svrishchuk alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT tadushenkova alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT asmokhov alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT mvdesyatova alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT eiermolenko alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT gfleontyeva alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT avsvarval alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT eeshchederkina alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT vvkolodzhieva alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT lyunilova alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT eaorishak alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies AT aegoncharov alteredmicrobiotainthefemalereproductivetractasariskfactorforfailureofassistedreproductivetechnologies
_version_	1718417921133248512

Altered microbiota in the female reproductive tract as a risk factor for failure of assisted reproductive technologies

Ejemplares similares