Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats

Abstract Retinitis pigmentosa (RP) is one of hereditary retinal diseases characterized by the loss of photoreceptors. Cell transplantation has been clinically applied to treat RP patients. Human retinal progenitor cells (HRPCs) and human bone marrow-derived mesenchymal stem cells (HBMSCs) are the tw...

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Autores principales:	Linghui Qu, Lixiong Gao, Haiwei Xu, Ping Duan, Yuxiao Zeng, Yong Liu, Zheng Qin Yin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6536fa8023ac4e988eadf4fdaa00a06e
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spelling	oai:doaj.org-article:6536fa8023ac4e988eadf4fdaa00a06e2021-12-02T16:08:12ZCombined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats10.1038/s41598-017-00241-52045-2322https://doaj.org/article/6536fa8023ac4e988eadf4fdaa00a06e2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00241-5https://doaj.org/toc/2045-2322Abstract Retinitis pigmentosa (RP) is one of hereditary retinal diseases characterized by the loss of photoreceptors. Cell transplantation has been clinically applied to treat RP patients. Human retinal progenitor cells (HRPCs) and human bone marrow-derived mesenchymal stem cells (HBMSCs) are the two commonly and practically used stem cells for transplantation. Since combined transplantation could be a promising way to integrate the advantages of both stem cell types, we transplanted HRPCs and HBMSCs into the subretinal space (SRS) of Royal College of Surgeons (RCS) rats. We report that HRPCs/HBMSCs combined transplantation maintains the electroretinogram results much better than HRPCs or HBMSCs single transplantations. The thickness of outer nuclear layer also presented a better outcome in the combined transplantation. Importantly, grafted cells in the combination migrated better, both longitudinally and latitudinally, than single transplantation. The photoreceptor differentiation of grafted cells in the retina of RCS rats receiving combined transplantation also showed a higher ratio than single transplantation. Finally, activation of microglia and the gliosis of Müller cells were more effectively suppressed in combined transplantation, indicating better immunomodulatory and anti-gliosis effects. Taken together, combining the transplantation of HRPCs and HBMSCs is a more effective strategy in stem cell-based therapy for retinal degenerative diseases.Linghui QuLixiong GaoHaiwei XuPing DuanYuxiao ZengYong LiuZheng Qin YinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Linghui Qu Lixiong Gao Haiwei Xu Ping Duan Yuxiao Zeng Yong Liu Zheng Qin Yin Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats
description	Abstract Retinitis pigmentosa (RP) is one of hereditary retinal diseases characterized by the loss of photoreceptors. Cell transplantation has been clinically applied to treat RP patients. Human retinal progenitor cells (HRPCs) and human bone marrow-derived mesenchymal stem cells (HBMSCs) are the two commonly and practically used stem cells for transplantation. Since combined transplantation could be a promising way to integrate the advantages of both stem cell types, we transplanted HRPCs and HBMSCs into the subretinal space (SRS) of Royal College of Surgeons (RCS) rats. We report that HRPCs/HBMSCs combined transplantation maintains the electroretinogram results much better than HRPCs or HBMSCs single transplantations. The thickness of outer nuclear layer also presented a better outcome in the combined transplantation. Importantly, grafted cells in the combination migrated better, both longitudinally and latitudinally, than single transplantation. The photoreceptor differentiation of grafted cells in the retina of RCS rats receiving combined transplantation also showed a higher ratio than single transplantation. Finally, activation of microglia and the gliosis of Müller cells were more effectively suppressed in combined transplantation, indicating better immunomodulatory and anti-gliosis effects. Taken together, combining the transplantation of HRPCs and HBMSCs is a more effective strategy in stem cell-based therapy for retinal degenerative diseases.
format	article
author	Linghui Qu Lixiong Gao Haiwei Xu Ping Duan Yuxiao Zeng Yong Liu Zheng Qin Yin
author_facet	Linghui Qu Lixiong Gao Haiwei Xu Ping Duan Yuxiao Zeng Yong Liu Zheng Qin Yin
author_sort	Linghui Qu
title	Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats
title_short	Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats
title_full	Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats
title_fullStr	Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats
title_full_unstemmed	Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats
title_sort	combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of rcs rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6536fa8023ac4e988eadf4fdaa00a06e
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Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats

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