Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle

Abstract Fat deposition of beef cattle varies between breeds. However, the regulation mechanism is still not elucidated completely at molecular level. In the present study, we comparatively analyzed transcriptome of subcutaneous adipose tissue between Wagyu and Holstein cattle with a significant dif...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wanlong Huang, Yuntao Guo, Weihua Du, Xiuxiu Zhang, Ai Li, Xiangyang Miao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/654737bec2a74a719e7496bc3c5373c9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:654737bec2a74a719e7496bc3c5373c9
record_format	dspace
spelling	oai:doaj.org-article:654737bec2a74a719e7496bc3c5373c92021-12-02T12:31:59ZGlobal transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle10.1038/s41598-017-05702-52045-2322https://doaj.org/article/654737bec2a74a719e7496bc3c5373c92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05702-5https://doaj.org/toc/2045-2322Abstract Fat deposition of beef cattle varies between breeds. However, the regulation mechanism is still not elucidated completely at molecular level. In the present study, we comparatively analyzed transcriptome of subcutaneous adipose tissue between Wagyu and Holstein cattle with a significant difference in fat deposition to identify key genes associated with fat metabolism and adipogenesis by high-throughput RNA-seq technology. A total of 59,149,852 and 69,947,982 high quality reads were generated, respectively. With further analysis, 662 differentially expressed genes were identified. Gene Ontology and KEGG pathway analysis revealed that many differentially expressed genes were enriched in several biological processes and pathways relevant to adipogenesis and lipid metabolism, in which PPAR and fatty acid metabolism signaling pathways with related genes such as PPARγ, PLIN2 and ELOVL6 et al. play a critical role. Protein-protein interaction network analysis showed EGR1, FOS, SERPINE1, AGT, MMP2 may have great impact on adipocyte differentiation and adipogenesis. Moreover, potential alternative splicing events and single nucleotide polymorphisms (SNPs) were also identified. In summary, we comprehensively analyzed and discussed the transcriptome of subcutaneous adipose tissue of Wagyu and Holstein cattle, which might provide a theoretical basis for better understanding molecular mechanism of fat metabolism and deposition in beef cattle.Wanlong HuangYuntao GuoWeihua DuXiuxiu ZhangAi LiXiangyang MiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wanlong Huang Yuntao Guo Weihua Du Xiuxiu Zhang Ai Li Xiangyang Miao Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle
description	Abstract Fat deposition of beef cattle varies between breeds. However, the regulation mechanism is still not elucidated completely at molecular level. In the present study, we comparatively analyzed transcriptome of subcutaneous adipose tissue between Wagyu and Holstein cattle with a significant difference in fat deposition to identify key genes associated with fat metabolism and adipogenesis by high-throughput RNA-seq technology. A total of 59,149,852 and 69,947,982 high quality reads were generated, respectively. With further analysis, 662 differentially expressed genes were identified. Gene Ontology and KEGG pathway analysis revealed that many differentially expressed genes were enriched in several biological processes and pathways relevant to adipogenesis and lipid metabolism, in which PPAR and fatty acid metabolism signaling pathways with related genes such as PPARγ, PLIN2 and ELOVL6 et al. play a critical role. Protein-protein interaction network analysis showed EGR1, FOS, SERPINE1, AGT, MMP2 may have great impact on adipocyte differentiation and adipogenesis. Moreover, potential alternative splicing events and single nucleotide polymorphisms (SNPs) were also identified. In summary, we comprehensively analyzed and discussed the transcriptome of subcutaneous adipose tissue of Wagyu and Holstein cattle, which might provide a theoretical basis for better understanding molecular mechanism of fat metabolism and deposition in beef cattle.
format	article
author	Wanlong Huang Yuntao Guo Weihua Du Xiuxiu Zhang Ai Li Xiangyang Miao
author_facet	Wanlong Huang Yuntao Guo Weihua Du Xiuxiu Zhang Ai Li Xiangyang Miao
author_sort	Wanlong Huang
title	Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle
title_short	Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle
title_full	Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle
title_fullStr	Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle
title_full_unstemmed	Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle
title_sort	global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in wagyu and holstein cattle
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/654737bec2a74a719e7496bc3c5373c9
work_keys_str_mv	AT wanlonghuang globaltranscriptomeanalysisidentifiesdifferentiallyexpressedgenesrelatedtolipidmetabolisminwagyuandholsteincattle AT yuntaoguo globaltranscriptomeanalysisidentifiesdifferentiallyexpressedgenesrelatedtolipidmetabolisminwagyuandholsteincattle AT weihuadu globaltranscriptomeanalysisidentifiesdifferentiallyexpressedgenesrelatedtolipidmetabolisminwagyuandholsteincattle AT xiuxiuzhang globaltranscriptomeanalysisidentifiesdifferentiallyexpressedgenesrelatedtolipidmetabolisminwagyuandholsteincattle AT aili globaltranscriptomeanalysisidentifiesdifferentiallyexpressedgenesrelatedtolipidmetabolisminwagyuandholsteincattle AT xiangyangmiao globaltranscriptomeanalysisidentifiesdifferentiallyexpressedgenesrelatedtolipidmetabolisminwagyuandholsteincattle
_version_	1718394217933307904

Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle

Ejemplares similares