Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells

Abstract Diabetic nephropathy (DN) is accompanied by characteristic changes in the glomerulus, but little is known about the effect of diabetes on parietal epithelial cells (PECs). In this study, a descriptive analysis of PECs was undertaken in diabetic db/db mice and in diabetic patients. PEC hyper...

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Autores principales:	Takahisa Kawaguchi, Kazuhiro Hasegawa, Itaru Yasuda, Hirokazu Muraoka, Hiroyuki Umino, Hirobumi Tokuyama, Akinori Hashiguchi, Shu Wakino, Hiroshi Itoh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/654968ad0bba462cb31abe2866a3e682
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spelling	oai:doaj.org-article:654968ad0bba462cb31abe2866a3e6822021-12-02T14:12:40ZDiabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells10.1038/s41598-021-81027-82045-2322https://doaj.org/article/654968ad0bba462cb31abe2866a3e6822021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81027-8https://doaj.org/toc/2045-2322Abstract Diabetic nephropathy (DN) is accompanied by characteristic changes in the glomerulus, but little is known about the effect of diabetes on parietal epithelial cells (PECs). In this study, a descriptive analysis of PECs was undertaken in diabetic db/db mice and in diabetic patients. PEC hypertrophy was significantly more prominent in diabetic mice than in nondiabetic mice, and this was evident even at the early stage. Additionally, the number of vacuoles in PECs was markedly increased in diabetic mice, suggesting the presence of cellular injury in PECs in DN. Although rare, binuclear cells were observed in mice with early diabetes. In cultured PECs, a high glucose condition, compared with normal glucose condition, induced cellular hypertrophy and apoptosis. Flow cytometry showed that some PECs in the G0 phase reentered the cell cycle but got arrested in the S phase. Finally, in human diabetic subjects, hypertrophy and vacuolization were observed in the PECs. Our data showed that PECs undergo substantial changes in DN and may participate in rearrangement for differentiation into podocytes.Takahisa KawaguchiKazuhiro HasegawaItaru YasudaHirokazu MuraokaHiroyuki UminoHirobumi TokuyamaAkinori HashiguchiShu WakinoHiroshi ItohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takahisa Kawaguchi Kazuhiro Hasegawa Itaru Yasuda Hirokazu Muraoka Hiroyuki Umino Hirobumi Tokuyama Akinori Hashiguchi Shu Wakino Hiroshi Itoh Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells
description	Abstract Diabetic nephropathy (DN) is accompanied by characteristic changes in the glomerulus, but little is known about the effect of diabetes on parietal epithelial cells (PECs). In this study, a descriptive analysis of PECs was undertaken in diabetic db/db mice and in diabetic patients. PEC hypertrophy was significantly more prominent in diabetic mice than in nondiabetic mice, and this was evident even at the early stage. Additionally, the number of vacuoles in PECs was markedly increased in diabetic mice, suggesting the presence of cellular injury in PECs in DN. Although rare, binuclear cells were observed in mice with early diabetes. In cultured PECs, a high glucose condition, compared with normal glucose condition, induced cellular hypertrophy and apoptosis. Flow cytometry showed that some PECs in the G0 phase reentered the cell cycle but got arrested in the S phase. Finally, in human diabetic subjects, hypertrophy and vacuolization were observed in the PECs. Our data showed that PECs undergo substantial changes in DN and may participate in rearrangement for differentiation into podocytes.
format	article
author	Takahisa Kawaguchi Kazuhiro Hasegawa Itaru Yasuda Hirokazu Muraoka Hiroyuki Umino Hirobumi Tokuyama Akinori Hashiguchi Shu Wakino Hiroshi Itoh
author_facet	Takahisa Kawaguchi Kazuhiro Hasegawa Itaru Yasuda Hirokazu Muraoka Hiroyuki Umino Hirobumi Tokuyama Akinori Hashiguchi Shu Wakino Hiroshi Itoh
author_sort	Takahisa Kawaguchi
title	Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells
title_short	Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells
title_full	Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells
title_fullStr	Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells
title_full_unstemmed	Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells
title_sort	diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/654968ad0bba462cb31abe2866a3e682
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Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells

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