Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations

Ectoine is one of the compatible organic molecules that can protect the protein from heating, freezing, and chemicals contact. This study aims to investigate the ability of ectoine to stabilize lipase on heating and in methanol treatments as an effort to provide a stable biocatalyst for the producti...

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Autores principales:	I Putu Parwata, Deana Wahyuningrum, Sony Suhandono, Rukman Hertadi
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2021
Materias:	ectoine lipase heat methanol protection Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/654a67e9bf3b491f8b0a24431c68fdc0
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spelling	oai:doaj.org-article:654a67e9bf3b491f8b0a24431c68fdc02021-12-02T15:58:52ZAbility of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations1411-94202460-157810.22146/ijc.54931https://doaj.org/article/654a67e9bf3b491f8b0a24431c68fdc02021-03-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/54931https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Ectoine is one of the compatible organic molecules that can protect the protein from heating, freezing, and chemicals contact. This study aims to investigate the ability of ectoine to stabilize lipase on heating and in methanol treatments as an effort to provide a stable biocatalyst for the production of biodiesel. Various ectoine concentrations were added to lipase solutions, then the mixture was heated, and the residual activity of the lipase was determined. Similar steps were also conducted for methanol treatment. The results showed that ectoine maintained and even improved the catalytic activity of lipase after treatment with either heat or methanol. The addition of ectoine to a final concentration of 110 to 150 mM could maintain lipase activity up to 80% when heating to approximately 95 °C. Additionally, more than 20% of lipase activity increased on heating to temperatures below 75 °C in the presence of ectoine at a final concentration of 25 to 120 mM. Meanwhile, after incubation in methanol at a level of around 84% (v/v), the activity of lipase containing 40–90 mM ectoine was maintained. These results demonstrated that ectoine was highly effective in protecting lipase from heat and methanol.I Putu ParwataDeana WahyuningrumSony SuhandonoRukman HertadiDepartment of Chemistry, Universitas Gadjah MadaarticleectoinelipaseheatmethanolprotectionChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 21, Iss 2, Pp 494-501 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ectoine lipase heat methanol protection Chemistry QD1-999
spellingShingle	ectoine lipase heat methanol protection Chemistry QD1-999 I Putu Parwata Deana Wahyuningrum Sony Suhandono Rukman Hertadi Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations
description	Ectoine is one of the compatible organic molecules that can protect the protein from heating, freezing, and chemicals contact. This study aims to investigate the ability of ectoine to stabilize lipase on heating and in methanol treatments as an effort to provide a stable biocatalyst for the production of biodiesel. Various ectoine concentrations were added to lipase solutions, then the mixture was heated, and the residual activity of the lipase was determined. Similar steps were also conducted for methanol treatment. The results showed that ectoine maintained and even improved the catalytic activity of lipase after treatment with either heat or methanol. The addition of ectoine to a final concentration of 110 to 150 mM could maintain lipase activity up to 80% when heating to approximately 95 °C. Additionally, more than 20% of lipase activity increased on heating to temperatures below 75 °C in the presence of ectoine at a final concentration of 25 to 120 mM. Meanwhile, after incubation in methanol at a level of around 84% (v/v), the activity of lipase containing 40–90 mM ectoine was maintained. These results demonstrated that ectoine was highly effective in protecting lipase from heat and methanol.
format	article
author	I Putu Parwata Deana Wahyuningrum Sony Suhandono Rukman Hertadi
author_facet	I Putu Parwata Deana Wahyuningrum Sony Suhandono Rukman Hertadi
author_sort	I Putu Parwata
title	Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations
title_short	Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations
title_full	Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations
title_fullStr	Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations
title_full_unstemmed	Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations
title_sort	ability of ectoine to stabilize lipase against elevated temperatures and methanol concentrations
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/654a67e9bf3b491f8b0a24431c68fdc0
work_keys_str_mv	AT iputuparwata abilityofectoinetostabilizelipaseagainstelevatedtemperaturesandmethanolconcentrations AT deanawahyuningrum abilityofectoinetostabilizelipaseagainstelevatedtemperaturesandmethanolconcentrations AT sonysuhandono abilityofectoinetostabilizelipaseagainstelevatedtemperaturesandmethanolconcentrations AT rukmanhertadi abilityofectoinetostabilizelipaseagainstelevatedtemperaturesandmethanolconcentrations
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Ability of Ectoine to Stabilize Lipase against Elevated Temperatures and Methanol Concentrations

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