Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo

Abstract Human herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-asso...

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Autores principales:	Gervillien Arnold Malonga, Aude Jary, Valentin Leducq, Dimitry Moudiongui Mboungou Malanda, Anicet Luc Magloire Boumba, Elodie Chicaud, Isabelle Malet, Vincent Calvez, Jean Felix Peko, Anne-Geneviève Marcelin
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Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/654c332483e24fba9a62f533769fb3ae
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spelling	oai:doaj.org-article:654c332483e24fba9a62f533769fb3ae2021-12-02T19:09:19ZSeroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo10.1038/s41598-021-97070-42045-2322https://doaj.org/article/654c332483e24fba9a62f533769fb3ae2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97070-4https://doaj.org/toc/2045-2322Abstract Human herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-associated nuclear antigen were detected by indirect immunofluorescence. In HHV-8 positive patients, we performed HHV-8 quantification in blood and saliva by real-time PCR and typing by Sanger sequencing of K1 open reading frame. HHV-8 seroprevalence was 19%, being male (odd ratio [OR] = 1.741, [95% Confidence interval {CI}, 0.97–3.07]; p = 0.0581) and having multiple sex partners before HIV diagnosis (OR = 1.682, [CI 95%, 0.97–2.92]; p = 0.0629) tended to be associated with HHV-8 seropositivity. Of the 64 HHV-8 seropositive patients, HHV-8 DNA was detected in 10 (16%) in saliva, 6 (9%) in whole-blood and in 2 (3%) in both whole-blood and saliva. Three out of 6 HHV-8 strains were subtypes A5, 2 subtype B1 and 1 subtype C. HHV-8 seroprevalence was relatively low with more frequent carriage in men, associated with asymptomatic oral excretion and a predominance of subtype A5. These data tend to support the hypothesis of horizontal transmission in people living with HIV in Brazzaville.Gervillien Arnold MalongaAude JaryValentin LeducqDimitry Moudiongui Mboungou MalandaAnicet Luc Magloire BoumbaElodie ChicaudIsabelle MaletVincent CalvezJean Felix PekoAnne-Geneviève MarcelinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gervillien Arnold Malonga Aude Jary Valentin Leducq Dimitry Moudiongui Mboungou Malanda Anicet Luc Magloire Boumba Elodie Chicaud Isabelle Malet Vincent Calvez Jean Felix Peko Anne-Geneviève Marcelin Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
description	Abstract Human herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-associated nuclear antigen were detected by indirect immunofluorescence. In HHV-8 positive patients, we performed HHV-8 quantification in blood and saliva by real-time PCR and typing by Sanger sequencing of K1 open reading frame. HHV-8 seroprevalence was 19%, being male (odd ratio [OR] = 1.741, [95% Confidence interval {CI}, 0.97–3.07]; p = 0.0581) and having multiple sex partners before HIV diagnosis (OR = 1.682, [CI 95%, 0.97–2.92]; p = 0.0629) tended to be associated with HHV-8 seropositivity. Of the 64 HHV-8 seropositive patients, HHV-8 DNA was detected in 10 (16%) in saliva, 6 (9%) in whole-blood and in 2 (3%) in both whole-blood and saliva. Three out of 6 HHV-8 strains were subtypes A5, 2 subtype B1 and 1 subtype C. HHV-8 seroprevalence was relatively low with more frequent carriage in men, associated with asymptomatic oral excretion and a predominance of subtype A5. These data tend to support the hypothesis of horizontal transmission in people living with HIV in Brazzaville.
format	article
author	Gervillien Arnold Malonga Aude Jary Valentin Leducq Dimitry Moudiongui Mboungou Malanda Anicet Luc Magloire Boumba Elodie Chicaud Isabelle Malet Vincent Calvez Jean Felix Peko Anne-Geneviève Marcelin
author_facet	Gervillien Arnold Malonga Aude Jary Valentin Leducq Dimitry Moudiongui Mboungou Malanda Anicet Luc Magloire Boumba Elodie Chicaud Isabelle Malet Vincent Calvez Jean Felix Peko Anne-Geneviève Marcelin
author_sort	Gervillien Arnold Malonga
title	Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_short	Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_full	Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_fullStr	Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_full_unstemmed	Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_sort	seroprevalence and molecular diversity of human herpesvirus 8 among people living with hiv in brazzaville, congo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/654c332483e24fba9a62f533769fb3ae
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Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo

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