Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease

Background: Kawasaki disease (KD) is a form of febrile vasculitis that primarily occurs in children. It can cause inflammation of the coronary arteries, which leads to aneurysms. The pathogenesis of coronary arteries may be associated with apoptosis or pyroptosis mediated by caspases activity, but t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kuang-Che Kuo, Ya-Ling Yang, Mao-Hung Lo, Xin-Yuan Cai, Mindy Ming-Huey Guo, Ho-Chang Kuo, Ying-Hsien Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Kawasaki disease coronary arterial lesions pyroptosis caspases IVIG resistance Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/654f1af350ba44bf88c83cb73b930c14
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:654f1af350ba44bf88c83cb73b930c14
record_format	dspace
spelling	oai:doaj.org-article:654f1af350ba44bf88c83cb73b930c142021-11-25T17:20:59ZIncreased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease10.3390/diagnostics111120352075-4418https://doaj.org/article/654f1af350ba44bf88c83cb73b930c142021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2035https://doaj.org/toc/2075-4418Background: Kawasaki disease (KD) is a form of febrile vasculitis that primarily occurs in children. It can cause inflammation of the coronary arteries, which leads to aneurysms. The pathogenesis of coronary arteries may be associated with apoptosis or pyroptosis mediated by caspases activity, but this idea has not been discussed much in KD. Materials and Methods: We enrolled 236 participants in this study. In the Affymetrix GeneChip<sup>®</sup> Human Transcriptome Array 2.0 study, there were 18 KD patients analyzed prior to receiving intravenous immunoglobulin (IVIG) treatment, at least 3 weeks after IVIG treatment, and 36 non-KD control subjects. We also recruited 24 KD patients prior to receiving IVIG treatment, at least 3 weeks after IVIG treatment, and 24 non-KD control subjects for Illumina HumanMethylation450 BeadChip study. A separate cohort of 134 subjects was analyzed to validate real-time quantitative PCR. Results: The mRNA levels of caspase-1, -3, -4, and -5 were significantly increased in KD patients compared with control subjects (<i>p</i> < 0.05). After administration of IVIG, the expression of these genes decreased considerably. Of particular note, the methylation status of the CpG sites of the caspase-4 and -5 genes demonstrated significant opposite tendencies between the KD patients and controls. Furthermore, compared with patients who responded to IVIG, refractory KD patients had a lower expression of the caspase-3 gene prior to IVIG treatment. Conclusion: Our study is the first to report the upregulation of pyroptotic caspase-1, -4, and -5 in peripheral leukocytes of KD patients. Moreover, the expression of caspase-3 may be associated with IVIG resistance in KD.Kuang-Che KuoYa-Ling YangMao-Hung LoXin-Yuan CaiMindy Ming-Huey GuoHo-Chang KuoYing-Hsien HuangMDPI AGarticleKawasaki diseasecoronary arterial lesionspyroptosiscaspasesIVIG resistanceMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2035, p 2035 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Kawasaki disease coronary arterial lesions pyroptosis caspases IVIG resistance Medicine (General) R5-920
spellingShingle	Kawasaki disease coronary arterial lesions pyroptosis caspases IVIG resistance Medicine (General) R5-920 Kuang-Che Kuo Ya-Ling Yang Mao-Hung Lo Xin-Yuan Cai Mindy Ming-Huey Guo Ho-Chang Kuo Ying-Hsien Huang Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease
description	Background: Kawasaki disease (KD) is a form of febrile vasculitis that primarily occurs in children. It can cause inflammation of the coronary arteries, which leads to aneurysms. The pathogenesis of coronary arteries may be associated with apoptosis or pyroptosis mediated by caspases activity, but this idea has not been discussed much in KD. Materials and Methods: We enrolled 236 participants in this study. In the Affymetrix GeneChip<sup>®</sup> Human Transcriptome Array 2.0 study, there were 18 KD patients analyzed prior to receiving intravenous immunoglobulin (IVIG) treatment, at least 3 weeks after IVIG treatment, and 36 non-KD control subjects. We also recruited 24 KD patients prior to receiving IVIG treatment, at least 3 weeks after IVIG treatment, and 24 non-KD control subjects for Illumina HumanMethylation450 BeadChip study. A separate cohort of 134 subjects was analyzed to validate real-time quantitative PCR. Results: The mRNA levels of caspase-1, -3, -4, and -5 were significantly increased in KD patients compared with control subjects (<i>p</i> < 0.05). After administration of IVIG, the expression of these genes decreased considerably. Of particular note, the methylation status of the CpG sites of the caspase-4 and -5 genes demonstrated significant opposite tendencies between the KD patients and controls. Furthermore, compared with patients who responded to IVIG, refractory KD patients had a lower expression of the caspase-3 gene prior to IVIG treatment. Conclusion: Our study is the first to report the upregulation of pyroptotic caspase-1, -4, and -5 in peripheral leukocytes of KD patients. Moreover, the expression of caspase-3 may be associated with IVIG resistance in KD.
format	article
author	Kuang-Che Kuo Ya-Ling Yang Mao-Hung Lo Xin-Yuan Cai Mindy Ming-Huey Guo Ho-Chang Kuo Ying-Hsien Huang
author_facet	Kuang-Che Kuo Ya-Ling Yang Mao-Hung Lo Xin-Yuan Cai Mindy Ming-Huey Guo Ho-Chang Kuo Ying-Hsien Huang
author_sort	Kuang-Che Kuo
title	Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease
title_short	Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease
title_full	Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease
title_fullStr	Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease
title_full_unstemmed	Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease
title_sort	increased expression of pyroptosis in leukocytes of patients with kawasaki disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/654f1af350ba44bf88c83cb73b930c14
work_keys_str_mv	AT kuangchekuo increasedexpressionofpyroptosisinleukocytesofpatientswithkawasakidisease AT yalingyang increasedexpressionofpyroptosisinleukocytesofpatientswithkawasakidisease AT maohunglo increasedexpressionofpyroptosisinleukocytesofpatientswithkawasakidisease AT xinyuancai increasedexpressionofpyroptosisinleukocytesofpatientswithkawasakidisease AT mindyminghueyguo increasedexpressionofpyroptosisinleukocytesofpatientswithkawasakidisease AT hochangkuo increasedexpressionofpyroptosisinleukocytesofpatientswithkawasakidisease AT yinghsienhuang increasedexpressionofpyroptosisinleukocytesofpatientswithkawasakidisease
_version_	1718412474415316992

Increased Expression of Pyroptosis in Leukocytes of Patients with Kawasaki Disease

Ejemplares similares