Quantitative conversion of biomass in giant DNA virus infection

Abstract Bioconversion of organic materials is the foundation of many applications in chemical engineering, microbiology and biochemistry. Herein, we introduce a new methodology to quantitatively determine conversion of biomass in viral infections while simultaneously imaging morphological changes o...

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Autores principales:	Mikael Kördel, Martin Svenda, Hemanth K. N. Reddy, Emelie Fogelqvist, Komang G. Y. Arsana, Bejan Hamawandi, Muhammet S. Toprak, Hans M. Hertz, Jonas A. Sellberg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6551d364f3ea4210a00d5cce1bf4bb5f
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spelling	oai:doaj.org-article:6551d364f3ea4210a00d5cce1bf4bb5f2021-12-02T13:20:02ZQuantitative conversion of biomass in giant DNA virus infection10.1038/s41598-021-83547-92045-2322https://doaj.org/article/6551d364f3ea4210a00d5cce1bf4bb5f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83547-9https://doaj.org/toc/2045-2322Abstract Bioconversion of organic materials is the foundation of many applications in chemical engineering, microbiology and biochemistry. Herein, we introduce a new methodology to quantitatively determine conversion of biomass in viral infections while simultaneously imaging morphological changes of the host cell. As proof of concept, the viral replication of an unidentified giant DNA virus and the cellular response of an amoebal host are studied using soft X-ray microscopy, titration dilution measurements and thermal gravimetric analysis. We find that virions produced inside the cell are visible from 18 h post infection and their numbers increase gradually to a burst size of 280–660 virions. Due to the large size of the virion and its strong X-ray absorption contrast, we estimate that the burst size corresponds to a conversion of 6–12% of carbonaceous biomass from amoebal host to virus. The occurrence of virion production correlates with the appearance of a possible viral factory and morphological changes in the phagosomes and contractile vacuole complex of the amoeba, whereas the nucleus and nucleolus appear unaffected throughout most of the replication cycle.Mikael KördelMartin SvendaHemanth K. N. ReddyEmelie FogelqvistKomang G. Y. ArsanaBejan HamawandiMuhammet S. ToprakHans M. HertzJonas A. SellbergNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mikael Kördel Martin Svenda Hemanth K. N. Reddy Emelie Fogelqvist Komang G. Y. Arsana Bejan Hamawandi Muhammet S. Toprak Hans M. Hertz Jonas A. Sellberg Quantitative conversion of biomass in giant DNA virus infection
description	Abstract Bioconversion of organic materials is the foundation of many applications in chemical engineering, microbiology and biochemistry. Herein, we introduce a new methodology to quantitatively determine conversion of biomass in viral infections while simultaneously imaging morphological changes of the host cell. As proof of concept, the viral replication of an unidentified giant DNA virus and the cellular response of an amoebal host are studied using soft X-ray microscopy, titration dilution measurements and thermal gravimetric analysis. We find that virions produced inside the cell are visible from 18 h post infection and their numbers increase gradually to a burst size of 280–660 virions. Due to the large size of the virion and its strong X-ray absorption contrast, we estimate that the burst size corresponds to a conversion of 6–12% of carbonaceous biomass from amoebal host to virus. The occurrence of virion production correlates with the appearance of a possible viral factory and morphological changes in the phagosomes and contractile vacuole complex of the amoeba, whereas the nucleus and nucleolus appear unaffected throughout most of the replication cycle.
format	article
author	Mikael Kördel Martin Svenda Hemanth K. N. Reddy Emelie Fogelqvist Komang G. Y. Arsana Bejan Hamawandi Muhammet S. Toprak Hans M. Hertz Jonas A. Sellberg
author_facet	Mikael Kördel Martin Svenda Hemanth K. N. Reddy Emelie Fogelqvist Komang G. Y. Arsana Bejan Hamawandi Muhammet S. Toprak Hans M. Hertz Jonas A. Sellberg
author_sort	Mikael Kördel
title	Quantitative conversion of biomass in giant DNA virus infection
title_short	Quantitative conversion of biomass in giant DNA virus infection
title_full	Quantitative conversion of biomass in giant DNA virus infection
title_fullStr	Quantitative conversion of biomass in giant DNA virus infection
title_full_unstemmed	Quantitative conversion of biomass in giant DNA virus infection
title_sort	quantitative conversion of biomass in giant dna virus infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6551d364f3ea4210a00d5cce1bf4bb5f
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_version_	1718393215539740672

Quantitative conversion of biomass in giant DNA virus infection

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